Label-free determination of protein-ligand binding constants using mass spectrometry and validation using surface plasmon resonance and isothermal titration calorimetry

2009 ◽  
Vol 22 (4) ◽  
pp. 319-329 ◽  
Author(s):  
Matthias C. Jecklin ◽  
Stefan Schauer ◽  
Christoph E. Dumelin ◽  
Renato Zenobi
Keyword(s):  
Mass Spectrometry ◽  
Surface Plasmon Resonance ◽  
Ligand Binding ◽  
Isothermal Titration Calorimetry ◽  
Surface Plasmon ◽  
Plasmon Resonance ◽  
Binding Constants ◽  
Titration Calorimetry ◽  
Label Free

Fragment Screening for the Modelling Community: SPR, ITC, and Crystallography

2013 ◽  
Vol 66 (12) ◽  
pp. 1507 ◽  
Author(s):  
Olan Dolezal ◽  
Larissa Doughty ◽  
Meghan K. Hattarki ◽  
Vincent J. Fazio ◽  
Tom T. Caradoc-Davies ◽  
...  
Keyword(s):  
Surface Plasmon Resonance ◽  
Isothermal Titration Calorimetry ◽  
Surface Plasmon ◽  
Plasmon Resonance ◽  
Titration Calorimetry ◽  
Small Fragment ◽  
Fragment Screening ◽  
Bovine Trypsin ◽  
Fragment Library ◽  
Biochemical Systems

The SAMPL (Statistical Assessment of the Modelling of Proteins and Ligands) challenge brought together experimentalists and modellers in an effort to improve our understanding of chemical and biochemical systems so better modelling tools can be developed. The most recent challenge, SAMPL3, held at Stanford University in August 2011, was an attempt to improve the methods used to predict how small fragment compounds bind to proteins, and the protein chosen for this test was bovine trypsin. Surface plasmon resonance was used to screen 500 compounds from a Maybridge fragment library and these compounds were subsequently used to soak crystals of trypsin and the best hits were also characterised by isothermal titration calorimetry. We present methods used for the surface plasmon resonance and the isothermal titration calorimetry experiments, as well as the results for these methods and those compounds that were found in the crystal structures.

SPRI biosensors for quantitative determination of matrix metalloproteinase-2

2017 ◽  
Vol 9 (16) ◽  
pp. 2407-2414 ◽  
Author(s):  
Anna Tokarzewicz ◽  
Lech Romanowicz ◽  
Iosif Sveklo ◽  
Ewa Matuszczak ◽  
Adam Hermanowicz ◽  
...  
Keyword(s):  
Surface Plasmon Resonance ◽  
Matrix Metalloproteinase ◽  
Quantitative Determination ◽  
Surface Plasmon ◽  
Plasmon Resonance ◽  
Matrix Metalloproteinase 2 ◽  
Surface Plasmon Resonance Imaging ◽  
Label Free ◽  
Resonance Imaging

The aim of this study was to develop a new, label-free, highly selective Surface Plasmon Resonance Imaging biosensor for the quantitative determination of matrix metalloproteinase-2.

scholarly journals Isothermal titration calorimetry and surface plasmon resonance analysis using the dynamic approach

2020 ◽  
Vol 21 ◽  
pp. 100712 ◽  
Author(s):  
Ganesh Kumar Krishnamoorthy ◽  
Prashanth Alluvada ◽  
Shahul Hameed Mohammed Sherieff ◽  
Timothy Kwa ◽  
Janarthanan Krishnamoorthy
Keyword(s):  
Surface Plasmon Resonance ◽  
Isothermal Titration Calorimetry ◽  
Surface Plasmon ◽  
Plasmon Resonance ◽  
Titration Calorimetry ◽  
Dynamic Approach ◽  
Surface Plasmon Resonance Analysis ◽  
Resonance Analysis
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