Expression, purification, and functional analysis of the C-terminal domain of Herbaspirillum seropedicae NifA protein

2003 ◽  
Vol 27 (2) ◽  
pp. 313-318 ◽  
Author(s):  
Rose A Monteiro ◽  
Emanuel M Souza ◽  
M Geoffrey Yates ◽  
M.Berenice R Steffens ◽  
Fábio O Pedrosa ◽  
...  
Keyword(s):  
Functional Analysis ◽  
Herbaspirillum Seropedicae ◽  
Terminal Domain ◽  
Nifa Protein

scholarly journals Structural and functional analysis of the phosphoryl transfer reaction mediated by the human small C-terminal domain phosphatase, Scp1

2010 ◽  
Vol 19 (5) ◽  
pp. 974-986 ◽  
Author(s):  
Mengmeng Zhang ◽  
June Liu ◽  
Youngjun Kim ◽  
Jack E. Dixon ◽  
Samuel L. Pfaff ◽  
...  
Keyword(s):  
Functional Analysis ◽  
Transfer Reaction ◽  
Phosphoryl Transfer ◽  
Terminal Domain

Use of lactose to induce expression of soluble NifA protein domains of Herbaspirillum seropedicae in Escherichia coli

2000 ◽  
Vol 46 (11) ◽  
pp. 1087-1090 ◽  
Author(s):  
Rose A. Monteiro ◽  
Emanuel M. Souza ◽  
M. Geoffrey Yates ◽  
Fábio O. Pedrosa ◽  
Leda S. Chubatsu
Keyword(s):  
Escherichia Coli ◽  
Protein Domains ◽  
Herbaspirillum Seropedicae ◽  
Nifa Protein

Functional Analysis of Human Smad1: Role of the Amino-Terminal Domain

1999 ◽  
Vol 258 (2) ◽  
pp. 366-373 ◽  
Author(s):  
Ren-He Xu ◽  
Robert J. Lechleider ◽  
Hsiu-Ming Shih ◽  
Chen-Fei Hao ◽  
Dvora Sredni ◽  
...  
Keyword(s):  
Functional Analysis ◽  
Amino Terminal ◽  
Terminal Domain ◽  
Amino Terminal Domain

scholarly journals Expression and functional analysis of an N-truncated NifA protein ofHerbaspirillum seropedicae

FEBS Letters ◽  
1999 ◽  
Vol 447 (2-3) ◽  
pp. 283-286 ◽  
Author(s):  
R.A Monteiro ◽  
E.M Souza ◽  
S Funayama ◽  
M.G Yates ◽  
F.O Pedrosa ◽  
...  
Keyword(s):  
Functional Analysis ◽  
Nifa Protein

scholarly journals In-transregulation of the N-truncated-NIFA protein ofHerbaspirillum seropedicaeby the N-terminal domain

1999 ◽  
Vol 180 (2) ◽  
pp. 157-161 ◽  
Author(s):  
Rose Adele Monteiro ◽  
Emanuel M Souza ◽  
M.Geoffrey Yates ◽  
Fábio O Pedrosa ◽  
Leda S Chubatsu
Keyword(s):  
Terminal Domain ◽  
Nifa Protein

scholarly journals Fnr Is Involved in Oxygen Control of Herbaspirillum seropedicae N-Truncated NifA Protein Activity in Escherichia coli

2003 ◽  
Vol 69 (3) ◽  
pp. 1527-1531 ◽  
Author(s):  
Rose A. Monteiro ◽  
Emanuel M. de Souza ◽  
M. Geoffrey Yates ◽  
Fabio O. Pedrosa ◽  
Leda S. Chubatsu
Keyword(s):  
Escherichia Coli ◽  
Protein A ◽  
Ammonium Ions ◽  
Oxygen Control ◽  
Herbaspirillum Seropedicae ◽  
Protein Activity ◽  
Iron Deprivation ◽  
Iron Requirement ◽  
Fnr Protein ◽  
Nifa Protein

ABSTRACT Herbaspirillum seropedicae is an endophytic diazotroph belonging to the β-subclass of the class Proteobacteria, which colonizes many members of the Gramineae. The activity of the NifA protein, a transcriptional activator of nif genes in H. seropedicae, is controlled by ammonium ions through its N-terminal domain and by oxygen through mechanisms that are not well understood. Here we report that the NifA protein of H. seropedicae is inactive and more susceptible to degradation in an fnr Escherichia coli background. Both effects correlate with oxygen exposure and iron deprivation. Our results suggest that the oxygen sensitivity and iron requirement for H. seropedicae NifA activity involve the Fnr protein.

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