nifa protein
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Author(s):  
A. A. Vladimirova ◽  
E. S. Akimova ◽  
An. K. Baymiev ◽  
Al. K. Baymiev

The functional specificity of the NifA protein within the group of nodule bacteria was studied.


2012 ◽  
Vol 45 (2) ◽  
pp. 113-117 ◽  
Author(s):  
C.Y. Nishikawa ◽  
L.M. Araújo ◽  
M.A.S. Kadowaki ◽  
R.A. Monteiro ◽  
M.B.R. Steffens ◽  
...  

2003 ◽  
Vol 69 (3) ◽  
pp. 1527-1531 ◽  
Author(s):  
Rose A. Monteiro ◽  
Emanuel M. de Souza ◽  
M. Geoffrey Yates ◽  
Fabio O. Pedrosa ◽  
Leda S. Chubatsu

ABSTRACT Herbaspirillum seropedicae is an endophytic diazotroph belonging to the β-subclass of the class Proteobacteria, which colonizes many members of the Gramineae. The activity of the NifA protein, a transcriptional activator of nif genes in H. seropedicae, is controlled by ammonium ions through its N-terminal domain and by oxygen through mechanisms that are not well understood. Here we report that the NifA protein of H. seropedicae is inactive and more susceptible to degradation in an fnr Escherichia coli background. Both effects correlate with oxygen exposure and iron deprivation. Our results suggest that the oxygen sensitivity and iron requirement for H. seropedicae NifA activity involve the Fnr protein.


2003 ◽  
Vol 27 (2) ◽  
pp. 313-318 ◽  
Author(s):  
Rose A Monteiro ◽  
Emanuel M Souza ◽  
M Geoffrey Yates ◽  
M.Berenice R Steffens ◽  
Fábio O Pedrosa ◽  
...  

FEBS Letters ◽  
2001 ◽  
Vol 508 (1) ◽  
pp. 1-4 ◽  
Author(s):  
Rose A. Monteiro ◽  
Emanuel M. de Souza ◽  
Roseli Wassem ◽  
M.Geoffrey Yates ◽  
Fabio O. Pedrosa ◽  
...  

2000 ◽  
Vol 46 (11) ◽  
pp. 1087-1090 ◽  
Author(s):  
Rose A Monteiro ◽  
Emanuel M Souza ◽  
M Geoffrey Yates ◽  
Fábio O Pedrosa ◽  
Leda S Chubatsu

Overexpression and purification are procedures used to allow functional and structural characterization of proteins. Many overexpressed proteins are partially or completely insoluble, and can not be easily purified. The NifA protein is an enhancer-binding protein involved in activating the expression of nif and some fix genes. The NifA protein from many organisms is usually insoluble when over-expressed, and therefore difficult to work with in vitro. In this work we have overexpressed the central+C-terminal and the central domains of the Herbaspirrilum seropedicae NifA protein in an Escherichia coli background. Expression was induced with either IPTG or lactose. The data showed that induction with lactose promoted a significantly higher percentage of these proteins in the soluble fraction than with IPTG. This probably reflects a slower kinetics of induction by lactose.Key words: Herbaspirillum seropedicae, NifA protein, transcriptional activator, nitrogen fixation, protein expression.


2000 ◽  
Vol 46 (11) ◽  
pp. 1087-1090 ◽  
Author(s):  
Rose A. Monteiro ◽  
Emanuel M. Souza ◽  
M. Geoffrey Yates ◽  
Fábio O. Pedrosa ◽  
Leda S. Chubatsu

1999 ◽  
Vol 180 (2) ◽  
pp. 157-161 ◽  
Author(s):  
Rose Adele Monteiro ◽  
Emanuel M Souza ◽  
M.Geoffrey Yates ◽  
Fábio O Pedrosa ◽  
Leda S Chubatsu
Keyword(s):  

FEBS Letters ◽  
1999 ◽  
Vol 447 (2-3) ◽  
pp. 283-286 ◽  
Author(s):  
R.A Monteiro ◽  
E.M Souza ◽  
S Funayama ◽  
M.G Yates ◽  
F.O Pedrosa ◽  
...  

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