Use of Dried Spots of Whole Blood, Plasma, and Mother's Milk Collected on Filter Paper for Measurement of Human Immunodeficiency Virus Type 1 Burden

2008 ◽  
Vol 2008 ◽  
pp. 351-352
Author(s):  
M.G. Bissell
Keyword(s):  
Human Immunodeficiency Virus ◽  
Blood Plasma ◽  
Human Immunodeficiency Virus Type ◽  
Virus Type ◽  
Filter Paper ◽  
Whole Blood ◽  
Immunodeficiency Virus ◽  
Mother's Milk ◽  
Mother’S Milk

scholarly journals Quantitation of Human Immunodeficiency Virus Type 1 RNA in Plasma by Using Blood Dried on Filter Paper

1998 ◽  
Vol 36 (1) ◽  
pp. 258-260 ◽  
Author(s):  
Susan A. Fiscus ◽  
Donald Brambilla ◽  
Lynell Grosso ◽  
Jody Schock ◽  
Michael Cronin
Keyword(s):  
Human Immunodeficiency Virus ◽  
Human Immunodeficiency Virus Type ◽  
Virus Type ◽  
Filter Paper ◽  
Whole Blood ◽  
Virus Rna ◽  
Immunodeficiency Virus ◽  
Standard Assay ◽  
Free Plasma

We compared whole blood dried on filter paper to the standard assay with frozen cell-free plasma for use in the quantitation of the human immunodeficiency virus RNA load in blood. RNA values from filter paper, corrected for the hematocrit, gave results comparable to those of the standard assay in terms of sensitivity and reproducibility.

scholarly journals Evaluation of the human immunodeficiency virus type 1 and 2 antibodies detection in dried whole blood spots (dbs) samples

2008 ◽  
Vol 50 (3) ◽  
pp. 151-156 ◽  
Author(s):  
Andréa Cauduro de Castro ◽  
Luiz Gustavo dos Anjos Borges ◽  
Ricardo da Silva de Souza ◽  
Melina Grudzinski ◽  
Pedro Alves D'Azevedo
Keyword(s):  
Human Immunodeficiency Virus ◽  
Human Immunodeficiency Virus Type ◽  
Virus Type ◽  
Whole Blood ◽  
Confirmatory Test ◽  
Blood Spots ◽  
Immunodeficiency Virus ◽  
Blood Spot ◽  
Antibodies Detection

Human Immunodeficiency Vírus Type 1 and 2 antibodies detection was performed in 457 dried whole blood spots samples (S&S 903). Q-Preven HIV 1+2 was the screening test used. The results were compared with the gold standard serum tests by ELISA (Cobas Core e Axsym HIV1/2 gO) and imunofluorescence was the definitive confirmatory test. The samples were obtained from the Hospital Nossa Senhora da Conceição in Porto Alegre, RS - Brazil, through whole blood transfer to filter paper card and sent to Caxias do Sul, RS - Brazil where the tests were performed. The dried whole blood spot stability was evaluated with two different panels. The first one was composed of five negative and five positive samples stored at room temperature, 4 ºC, -20 ºC and -70 ºC, while the second was composed of two negative and three positive samples stored at 37 ºC (humidity <50%). Each sample was screened every week for six weeks. These measurement results didn't show variation during the study period. The detected sensibility was 100%, specificity was 99.6%, the positive predictive value was 99.5% and negative predictive values were 100%. The results demonstrated high performance characteristics, opening a new perspective of dried whole blood spot utilization in HIV screening diagnosis.

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