Choleretic action of diosgenin is based upon the increases in canalicular membrane fluidity and transporter activity mediating bile acid independent bile flow

2003 ◽  
Vol 25 (3) ◽  
pp. 287-295 ◽  
Author(s):  
A Yamaguchi
Keyword(s):  
Bile Acid ◽  
Membrane Fluidity ◽  
Bile Flow ◽  
Canalicular Membrane

Choleretic action of diosgenin is based upon the increases in canalicurar membrane fluidity and transporter activity mediating bile acid independent bile flow

2001 ◽  
Vol 120 (5) ◽  
pp. A549-A549
Author(s):  
A YAMAGUCHI ◽  
S TAZUMA ◽  
H OCHI ◽  
T NISHIOKA ◽  
S YASUMIBA ◽  
...  
Keyword(s):  
Bile Acid ◽  
Membrane Fluidity ◽  
Bile Flow

Choleretic action of diosgenin is based upon the increases in canalicurar membrane fluidity and transporter activity mediating bile acid independent bile flow

2001 ◽  
Vol 120 (5) ◽  
pp. A549
Author(s):  
Atsushi Yamaguchi ◽  
Susumu Tazuma ◽  
Hidenori Ochi ◽  
Tomoji Nishioka ◽  
Sigeyuki Yasumiba ◽  
...  
Keyword(s):  
Bile Acid ◽  
Membrane Fluidity ◽  
Bile Flow

scholarly journals A Nuclear Receptor-mediated Choleretic Action of Fibrates is Associated with Enhanced Canalicular Membrane Fluidity and Transporter Activity Mediating Bile Acid-independent Bile Secretion

2005 ◽  
Vol 12 (4) ◽  
pp. 211-217 ◽  
Author(s):  
Tomoji Nishioka ◽  
Hideyuki Hyogo ◽  
Yoshihiro Numata ◽  
Atsushi Yamaguchi ◽  
Toshiya Kobuke ◽  
...  
Keyword(s):  
Bile Acid ◽  
Membrane Fluidity ◽  
Nuclear Receptor ◽  
Bile Secretion ◽  
Canalicular Membrane

Protoporphyrin IX-Induced Impairment of Biliary Lipid Secretion in the Rat

1989 ◽  
Vol 77 (5) ◽  
pp. 473-478 ◽  
Author(s):  
F. Perez-Barriocanal ◽  
J. G. Redondo-Torres ◽  
G. R. Villanueva ◽  
E. Arteche ◽  
M. M. Berenson ◽  
...  
Keyword(s):  
Body Weight ◽  
Bile Acid ◽  
Bile Flow ◽  
Acid Output ◽  
Total Phospholipid ◽  
Protoporphyrin Ix ◽  
Biliary Secretion ◽  
Canalicular Membrane ◽  
Anaesthetized Rats ◽  
Phospholipid Species

1. In order to gain information on the effect of protoporphyrin IX on changes in the properties of the canalicular plasma membrane, we studied the release of canalicular membrane constituents, namely phospholipids, cholesterol and 5′-nucleotidase, into bile in anaesthetized rats receiving saline or taurocholate (0.5 μmol min−1 100 g−1 body weight) with or without protoporphyrin IX infusion (10 or 20 μg min−1 100 g−1 body weight). 2. Protoporphyrin IX induced an impairment of spontaneous bile flow and of biliary secretion of cholesterol, phospholipids and bile acids. The taurocholate-induced increase in bile acid output was not significantly reduced by protoporphyrin IX at either of the doses used. However, when a cholestatic dose of protoporphyrin IX was infused, the taurocholate-induced bile flow and secretion of lecithin and cholesterol were significantly reduced. 3. Biliary output of phospholipid species other than lecithin did not counterbalance the protoporphyrin IX-induced reduction in biliary lecithin secretion. Biliary outputs of both total phospholipid and lecithin were inhibited by protoporphyrin IX to similar extents. 4. Protoporphyrin IX alone had no effect on the biliary release of 5′-nucleotidase, whereas when it was given with taurocholate, it increased the bile acid-induced biliary output of this enzyme markedly. 5. In summary, these results indicate that protoporphyrin IX impairs the biliary secretion of phospholipids and cholesterol but not that of bile acid. The release of canalicular membrane constituents other than lipids was also modified by protoporphyrin IX.

Permeation patterns of polar nonelectrolytes across the guinea pig biliary tree

1984 ◽  
Vol 247 (5) ◽  
pp. G527-G536 ◽  
Author(s):  
N. Tavoloni
Keyword(s):  
Steady State ◽  
Bile Acid ◽  
Bile Duct ◽  
Guinea Pig ◽  
Bile Acids ◽  
Bile Flow ◽  
Biliary Tree ◽  
Canalicular Membrane ◽  
Biliary Clearance ◽  
Spontaneous Secretion

The biliary permeation of polar nonelectrolytes was studied in anesthetized, bile duct-cannulated guinea pigs with functional cholecystectomy and nephrectomy. During spontaneous secretion, the steady-state bile-to-plasma ratio (B/P) of [14C]urea, [14C]erythritol, [14C]mannitol, [3H]sucrose, and [3H]inulin was 1.02, 0.90, 0.38, 0.12, and 0.04, respectively. Differently structured hydroxy bile acids, but not taurodehydrocholate, reversibly diminished [14C]erythritol and [14C]mannitol B/P during choleresis, and with some of them, particularly taurocholate and glycochenodeoxycholate, the biliary clearance of either solute declined below precholeretic levels. For any given hydroxy bile acid, the degree of B/P diminution was directly related to the molecular radii of these two inert carbohydrates. All bile acids failed to decrease [14C]urea, [3H]sucrose, and [3H]inulin B/P. On the contrary, most of them irreversibly increased [3H]sucrose and [3H]inulin permeability. These results suggest that in the guinea pig 1) hydroxy bile acids diminish the size or rearrange the architecture of the canalicular membrane "aqueous pores" through which [14C]erythritol and [14C]mannitol enter the canaliculus, and 2) solutes of the size of or smaller than [14C]mannitol enter bile primarily through a transcellular route, whereas [3H]sucrose, and [3H]inulin permeate mainly via a transjunctional shunt pathway. These studies indicate that [14C]erythritol and [14C]mannitol cannot be used to estimate canalicular bile flow in this species.

Liver cell membrane solubilization may control maximum secretory rate of cholic acid in the rat

1987 ◽  
Vol 252 (1) ◽  
pp. G84-G91 ◽  
Author(s):  
I. M. Yousef ◽  
S. Barnwell ◽  
F. Gratton ◽  
B. Tuchweber ◽  
A. Weber ◽  
...  
Keyword(s):  
Fatty Acid Composition ◽  
Fatty Acid ◽  
Bile Acid ◽  
Cholic Acid ◽  
Liver Cell ◽  
Bile Flow ◽  
Secretory Rate ◽  
Acid Infusion ◽  
Canalicular Membrane ◽  
Bile Canalicular Membrane

The factors modulating the maximum secretory rate of cholic acid were investigated. Rats were infused intravenously with cholic acid in measured stepwise increasing doses (1, 2, 3, and 4 mumol X min-1 X 100 g body wt-1). Each dose was infused for 30 min and bile samples were collected every 10 min. Bile flow, bile acid, cholesterol, individual biliary phospholipids, and the fatty acid profiles of the biliary phospholipids were determined. Microsomal and bile canalicular membrane-enriched fractions were isolated from cholic acid-treated rats at the end of the experiment. Membranes were analyzed for cholesterol, phospholipid, and phospholipid fatty acid composition. During cholic acid infusion, the secretion rates of bile acid, cholesterol, phospholipid, and bile flow initially increased and then declined. No evidence of liver cell damage was observed by light or electron microscopy. Maximum phospholipid secretion rate (13.5 nmol X min-1 X g-1) occurred before peak bile flow and bile acid secretory rate maximum (4.72 microliter X min-1 X g-1 and 375 nmol X min-1 X g-1). When phospholipid output declined, the proportion of sphingomyelins and phosphatidylethanolamine relative to phosphatidylcholine increased. This was also reflected in the fatty acid composition. Cholic acid infusion caused a decline in microsomal and bile canalicular membrane phospholipid content without affecting their phospholipid composition. Depletion of membrane phospholipid resulted in an increase in the cholesterol:phospholipid ratio, which is suggested to be the underlying mechanism for modulating cholic acid secretion.

scholarly journals Short- and long-term effects of biliary drainage on hepatic cholesterol metabolism in the rat

1990 ◽  
Vol 269 (3) ◽  
pp. 781-788 ◽  
Author(s):  
M J Smit ◽  
A M Temmerman ◽  
R Havinga ◽  
F Kuipers ◽  
R J Vonk
Keyword(s):  
Bile Acid ◽  
Bile Flow ◽  
Cholesterol Metabolism ◽  
Acid Output ◽  
Biliary Secretion ◽  
Surgical Trauma ◽  
Hepatic Cholesterol ◽  
Long Term Effects ◽  
Short And Long Term

The present study concerns short- and long-term effects of interruption of the enterohepatic circulation (EHC) on hepatic cholesterol metabolism and biliary secretion in rats. For this purpose, we employed a technique that allows reversible interruption of the EHC, during normal feeding conditions, and excludes effects of anaesthesia and surgical trauma. [3H]Cholesteryl oleate-labelled human low-density lipoprotein (LDL) was injected intravenously in rats with (1) chronically (8 days) interrupted EHC, (2) interrupted EHC at the time of LDL injection and (3) intact EHC. During the first 3 h after interruption of the EHC, bile flow decreased to 50% and biliary bile acid, phospholipid and cholesterol secretion to 5%, 11% and 19% of their initial values respectively. After 8 days of bile diversion, biliary cholesterol output and bile flow were at that same level, but bile acid output was increased 2-3-fold and phospholipid output was about 2 times lower. The total amount of cholesterol in the liver decreased after interruption of the EHC, which was mainly due to a decrease in the amount of cholesteryl ester. Plasma disappearance of LDL was not affected by interruption of the EHC. Biliary secretion of LDL-derived radioactivity occurred 2-4 times faster in chronically interrupted rats as compared with the excretion immediately after interruption of the EHC. Radioactivity was mainly in the form of bile acids under both conditions. This study demonstrates the very rapid changes that occur in cholesterol metabolism and biliary lipid composition after interruption of the EHC. These changes must be taken into account in studies concerning hepatic metabolism of lipoprotein cholesterol and subsequent secretion into bile.

Hepatocelluar cytoprotection by hydrophilic bile salts is through enhancing canalicular membrane fluidity and packing density

2000 ◽  
Vol 118 (4) ◽  
pp. A1422
Author(s):  
Yasumasa Asamoto ◽  
Susumu Tazuma ◽  
Hidenori Ochi ◽  
Tsuyoshi Kajihara ◽  
Hideyuki Hyougo ◽  
...  
Keyword(s):  
Membrane Fluidity ◽  
Packing Density ◽  
Bile Salts ◽  
Canalicular Membrane

Cyclic GMP modulates bile acid independent bile flow

Hepatology ◽  
1993 ◽  
Vol 18 (4) ◽  
pp. A135 ◽  
Author(s):  
M STPIERRE
Keyword(s):  
Bile Acid ◽  
Cyclic Gmp ◽  
Bile Flow
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