scholarly journals 368. Expression of a Human Codon-Optimized Bacteriophage T4 Endonuclease V Gene Enhances UV Resistance of Xeroderma Pigmentosum Group A Cells

2003 ◽  
Vol 7 (5) ◽  
pp. S144
1999 ◽  
Vol 27 (15) ◽  
pp. 3153-3158 ◽  
Author(s):  
L. Leonora J. ◽  
N. Hoehr ◽  
S. J. Mazur ◽  
G. L. Dianov ◽  
S. Senturker ◽  
...  

1992 ◽  
Vol 18 (4) ◽  
pp. 371-379 ◽  
Author(s):  
Gursurinder P. Kaur ◽  
Augustinus Rinaldy ◽  
R. Stephen Lloyd ◽  
Raghbir S. Athwal

1993 ◽  
Vol 13 (2) ◽  
pp. 970-976 ◽  
Author(s):  
L Lommel ◽  
P C Hanawalt

A UV-resistant revertant (XP129) of a xeroderma pigmentosum group A cell line has been reported to be totally deficient in repair of cyclobutane pyrimidine dimers (CPDs) but proficient in repair of 6-4 photoproducts. This finding has been interpreted to mean that CPDs play no role in cell killing by UV. We have analyzed the fine structure of repair of CPDs in the dihydrofolate reductase gene in the revertant. In this essential, active gene, we observe that repair of the transcribed strand is as efficient as that in normal, repair-proficient human cells, but repair of the nontranscribed strand is not. Within 4 h after UV at 7.5 J/m2, over 50% of the CPDs were removed, and by 8 h, 80% of the CPDs were removed. In contrast, there was essentially no removal from the nontranscribed strand even by 24 h. Our results demonstrate that overall repair measurements can be misleading, and they support the hypothesis that removal of CPDs from the transcribed strands of expressed genes is essential for UV resistance.


1990 ◽  
Vol 16 (4) ◽  
pp. 395-400 ◽  
Author(s):  
Karla A. Henning ◽  
Roger A. Schultz ◽  
Gurbax S. Sekhon ◽  
Errol C. Friedberg

1993 ◽  
Vol 13 (2) ◽  
pp. 970-976
Author(s):  
L Lommel ◽  
P C Hanawalt

A UV-resistant revertant (XP129) of a xeroderma pigmentosum group A cell line has been reported to be totally deficient in repair of cyclobutane pyrimidine dimers (CPDs) but proficient in repair of 6-4 photoproducts. This finding has been interpreted to mean that CPDs play no role in cell killing by UV. We have analyzed the fine structure of repair of CPDs in the dihydrofolate reductase gene in the revertant. In this essential, active gene, we observe that repair of the transcribed strand is as efficient as that in normal, repair-proficient human cells, but repair of the nontranscribed strand is not. Within 4 h after UV at 7.5 J/m2, over 50% of the CPDs were removed, and by 8 h, 80% of the CPDs were removed. In contrast, there was essentially no removal from the nontranscribed strand even by 24 h. Our results demonstrate that overall repair measurements can be misleading, and they support the hypothesis that removal of CPDs from the transcribed strands of expressed genes is essential for UV resistance.


2012 ◽  
Vol 66 (3) ◽  
pp. 242-244 ◽  
Author(s):  
Shinichi Moriwaki ◽  
Yoshito Takahashi ◽  
Hiroko Shimizu ◽  
Mayumi Inoue ◽  
Yoshinori Sugiyama ◽  
...  

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