scholarly journals Direct complement fixation test with avian infectious bronchitis virus in chickens

1970 ◽  
Vol 68 (2) ◽  
pp. 211-219 ◽  
Author(s):  
P. K. Uppal
Keyword(s):  
Infectious Bronchitis Virus ◽  
Neutralizing Antibodies ◽  
Complement Fixation Test ◽  
Complement Fixation ◽  
Allantoic Fluid ◽  
Fixation Test ◽  
Avian Infectious Bronchitis Virus ◽  
Infectious Bronchitis ◽  
Primary Inoculation ◽  
Serum Neutralization Test

SUMMARYThe direct complement fixation test was performed to follow the antibody response in chickens infected with avian infectious bronchitis virus. Concentrated allantoic fluid (4 units) was used as an antigen and allowed to react with serially diluted antiserum in the presence of two complete units of guinea-pig complement for 3 hr. at 4°C. and ½ hr. at 37°C. before the addition of sensitized cells. Serum was unheated and used either fresh or within one month of storage at −30°C. Individual birds showed a rise and fall of complement-fixing antibody both after primary and secondary inoculations. The complement-fixing antibody was detected as early as the seventh day after primary inoculation. The highest complement fixation titre (1/32 to 1/64) was recorded from 14 to 21 days after inoculation with a subsequent gradual decline.The results of the direct complement fixation tests have been correlated with the serum neutralization test. The neutralizing antibodies usually appeared by the 14th day but were not detected at a significant titre until the 21st day after primary inoculation. Serum neutralizing antibodies were still present at high titres even after 7 weeks of infection but the complement-fixing antibodies had disappeared by that time.

scholarly journals Preliminary Characterization of D?Aguilar Virus and Three Palyam Group Viruses New to Australia

1982 ◽  
Vol 35 (3) ◽  
pp. 343 ◽  
Author(s):  
DH Cybinski ◽  
TD St George
Keyword(s):  
Neutralizing Antibodies ◽  
Complement Fixation Test ◽  
Complement Fixation ◽  
Animal Health ◽  
Fixation Test ◽  
Preliminary Characterization ◽  
Restricted Distribution ◽  
Distribution Limits ◽  
Type Strains

Between 1974 and 1980, 424 viruses were isolated at the Long Pocket Laboratories of the Division of Animal Health, CSIRO, either from insects or from the blood of sentinel cattle, and of these, 165 cross-reacted with D'Aguilar virus (an Australian Palyam group virus) in a complement fixation test. Neutralization tests were used to classify these viruses into four serotypes with the isolates D'Aguilar B8112, CSIRO 11, CSIRO 58 and CSIRO 82 as the type strains. The latter three were new to Australia. Like other orbiviruses, these four serotypes were partially sensitive to treatment with ether or chloroform. Neutralizing antibodies against D' Aguilar, CSIRO 11 and CSIRO 58 viruses were detected in sera from cattle, buffalo, deer and sheep but not in sera from humans, horses, pigs or marsupials. Antibodies against CSIRO 82 virus were detected in 85 % of 26 buffalo, and O� 4 % of 495 cattle sera tested. The antibody distribution in Australia for D' Aguilar, CSIRO 11 and CSIRO 58 viruses fell within the distribution limits of Culicoides brevitarsis, the insect from which these viruses were most commonly recovered. The antibody distribution for CSIRO 82 virus, which was isolated from a pool containing C. schultze; and C. peregrinus, fell within the much more restricted distribution limits of these species. None of these viruses has been associated with disease.

Evaluation of the Complement Fixation Test in Amebiasis

1952 ◽  
Vol 21 (3) ◽  
pp. 391-399 ◽  
Author(s):  
Elwood Buchman ◽  
Harold J. Kullman ◽  
George F. Margonis
Keyword(s):  
Complement Fixation Test ◽  
Complement Fixation ◽  
Fixation Test

IMMUNOASSAY OF GONADOTROPHINS USING COMPLEMENT FIXATION

1969 ◽  
Vol 62 (1_Suppl) ◽  
pp. S113-S133 ◽  
Author(s):  
Sam Brody
Keyword(s):  
Complement Fixation Test ◽  
Complement Fixation ◽  
Research Work ◽  
Fixation Test ◽  
Years Of Experience ◽  
Standard Practice ◽  
Clinical Observations ◽  
Technical Procedure ◽  
Methodological Problems

ABSTRACT This report is a summary of 10 years of experience with the complement fixation test as adopted for the immunoassay of HCG in serum. It is based on published as well as unpublished material. The discussion centers mainly around methodological problems, criteria of reliability, and clinical observations. It is our impression that the complement fixation test is a reasonably rapid and simple technical procedure. It is standard practice in every bacteriological and virological laboratory. The precision of the HCG assay is high. Its accuracy is good. The complement fixation assay, as reported here, fulfils the criteria of specificity. It has been evaluated by means of serological techniques and through comparison between biopotency and immunopotency of HCG in serum with reference to a common standard. Its application for routine as well as research work is illustrated.

Rapid detection of Chlamydia/Chlamydophila group in samples collected from swine herds with and without reproductive disorders

2014 ◽  
Vol 17 (2) ◽  
pp. 367-369 ◽  
Author(s):  
K. Rypula ◽  
A. Kumala ◽  
P. Lis ◽  
K. Niemczuk ◽  
K. Płoneczka-Janeczko ◽  
...  
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Rapid Detection ◽  
Complement Fixation Test ◽  
Complement Fixation ◽  
Fixation Test ◽  
Reproductive Disorders ◽  
Serum Samples ◽  
Swine Herds

Abstract The study was carried out in seven reproductive herds of pigs. In three of them reproductive disorders were observed. Three herds consisted of 10-50 and four consisted of 120-500 adult sows and they were called small and medium, respectively. Fifty-seven adult sows were randomly selected from herds. Serum samples were tested using the complement fixation test and swabs from both eyes and from the vaginal vestibule were examined using real-time PCR. All serum samples were negative. Infected sows were present in each of the study herds. In total, there were 28 positive samples (53%, 28/48) in real-time PCR in sows with reproductive disorders and 35 (53%, 35/66) in sows selected from herds without problems in reproduction. One isolate proved to be Chlamydophila pecorum, whereas all the remaining were Chamydia suis

Comparative Evaluation of In Vitro and In Vivo Assays for the Detection of Avian Infectious Bronchitis Virus as a Contaminant of Live Poultry Vaccines

1998 ◽  
Vol 26 (5) ◽  
pp. 629-634
Author(s):  
Emiliana Falcone ◽  
Edoardo Vignolo ◽  
Livia Di Trani ◽  
Simona Puzelli ◽  
Maria Tollis
Keyword(s):  
Infectious Bronchitis Virus ◽  
Serological Response ◽  
Avian Infectious Bronchitis Virus ◽  
Animal Testing ◽  
Rt Pcr ◽  
Pcr Assay ◽  
In Vivo Test ◽  
Infectious Bronchitis

A reverse transcriptase polymerase chain reaction (RT-PCR) assay specific for identifying avian infectious bronchitis virus (IBV) in poultry vaccines, and the serological response to IBV induced by the inoculation of chicks with a Newcastle disease vaccine spiked with the Massachusetts strain of IBV, were compared for their ability to detect IBV as a contaminant of avian vaccines. The sensitivity of the IBV-RT-PCR assay provided results which were at least equivalent to the biological effect produced by the inoculation of chicks, allowing this assay to be considered a valid alternative to animal testing in the quality control of avian immunologicals. This procedure can easily be adapted to detect a number of contaminants for which the in vivo test still represents the only available method of detection.

Assessment of a complement fixation test to detect Mycoplasma hyopneumoniae infection in pigs

1984 ◽  
Vol 61 (7) ◽  
pp. 216-218
Author(s):  
L. C. LLOYD ◽  
R. T. BADMAN ◽  
J. R. ETHERIDGE ◽  
K. McKECHNIE ◽  
H. IYER
Keyword(s):  
Complement Fixation Test ◽  
Complement Fixation ◽  
Mycoplasma Hyopneumoniae ◽  
Fixation Test
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