Description of a tandem repeated DNA sequence of Echinostoma caproni and methods for its detection in snail and plankton samples

Parasitology ◽  
2003 ◽  
Vol 126 (5) ◽  
pp. 443-449 ◽  
Author(s):  
J. HERTEL ◽  
B. HABERL ◽  
J. HAMBURGER ◽  
W. HAAS
Keyword(s):  
Dna Sequence ◽  
High Specificity ◽  
Pcr Primers ◽  
Repeated Dna ◽  
Echinostoma Caproni ◽  
Larval Stages ◽  
Ecological Principles ◽  
Repeated Dna Sequence ◽  
Dna 2 ◽  
Snail Tissue

Echinostome larval stages in the snail have a great potential as effective competitors for the control of schistosomes and adult worms can cause painful intestinal diseases in humans. Ecology and transmission of the larval stages of trematodes are poorly understood, especially because their identification in field-collected samples by microscopy is difficult. We cloned, sequenced and analysed a 192 bp tandem repreated DNA sequence of Echinostoma caproni (EcSau3A), an often discussed antagonist of Schistosoma mansoni in Biomphalaria snails. PCR primers against this sequence can detect less than 10 fg of E. caproni DNA, 2 miracidia in snails 1 day p.i., 1 metacercaria in 50 mg snail tissue and 1 cercaria in 50 mg plankton with high specificity. Methods described in this study can support the discovery of fundamental ecological principles on distribution, host specificity and epidemiology of E. caproni larvae under field conditions.

A highly repeated DNA sequence in Arabidopsis thaliana

1986 ◽  
Vol 204 (3) ◽  
pp. 417-423 ◽  
Author(s):  
Jose M. Martinez-Zapater ◽  
Mark A. Estelle ◽  
Chris R. Somerville
Keyword(s):  
Arabidopsis Thaliana ◽  
Dna Sequence ◽  
Repeated Dna ◽  
Highly Repeated Dna ◽  
Repeated Dna Sequence

A new pericentromeric repeated DNA sequence in Microtus thomasi

2009 ◽  
Vol 124 (1) ◽  
pp. 27-36 ◽  
Author(s):  
M.J. Acosta ◽  
J.A. Marchal ◽  
G.P. Mitsainas ◽  
M.T. Rovatsos ◽  
C.H. Fernández-Espartero ◽  
...  
Keyword(s):  
Dna Sequence ◽  
Repeated Dna ◽  
Repeated Dna Sequence

scholarly journals Organization ofPlasmodium falciparum genome: 1. Evidence for a highly repeated DNA sequence

1985 ◽  
Vol 13 (6) ◽  
pp. 1965-1975 ◽  
Author(s):  
Ramareddy V. Guntaka ◽  
Siddarame Gowda ◽  
Arepalli S. Rao ◽  
Theodore J. Green
Keyword(s):  
Dna Sequence ◽  
Falciparum Genome ◽  
Repeated Dna ◽  
Highly Repeated Dna ◽  
Ofplasmodium Falciparum ◽  
Repeated Dna Sequence

Characterization of a major tandemly repeated DNA sequence (RBMII) prevalent among many species of waterfowl (Anatidae)

Genome ◽  
1992 ◽  
Vol 35 (6) ◽  
pp. 1037-1044 ◽  
Author(s):  
Cort S. Madsen ◽  
Kevin P. McHugh ◽  
Siwo R. de Kloet
Keyword(s):  
Dna Sequence ◽  
Sequence Variation ◽  
Sequence Divergence ◽  
Repeated Dna ◽  
Sequence Comparisons ◽  
Nonrandom Distribution ◽  
Repeated Dna Sequence ◽  
Tandemly Repeated Dna Sequence ◽  
Tandemly Repeated Dna

We have investigated the evolution of a 190 base pair tandemly repeated DNA sequence (RBMII) in 27 different species of waterfowl. In this paper we show that the RBMII sequence is present in many species belonging to 7 of the 11 Anatid tribes. Inter- and intra-tribal differences in repeat presence indicate that, although the RBMII sequence has been maintained among widely divergent species, it is rapidly evolving. Restriction enzyme analyses suggest very different hierarchical repeat organizations among different species. DNA sequence comparisons of 32 cloned monomer units from five different species revealed what appears to be a nonrandom distribution of sequence divergence, as well as large differences (up to 25-fold) in intraspecific sequence variation between relatively closely related species.Key words: repeated DNA, Anatidae, sequence variation.

A moderately repeated DNA sequence of wheat and rye genomes

1992 ◽  
Vol 18 (3) ◽  
pp. 603-605 ◽  
Author(s):  
Marta Dobrzańska ◽  
Blanka Szurmak
Keyword(s):  
Dna Sequence ◽  
Repeated Dna ◽  
Repeated Dna Sequence

Organization and distribution of a Sau3A tandem repeated DNA sequence in Picea (Pinaceae) species

Genome ◽  
1998 ◽  
Vol 41 (4) ◽  
pp. 560-565 ◽  
Author(s):  
Garth R Brown ◽  
Craig H Newton ◽  
John E Carlson
Keyword(s):  
In Situ Hybridization ◽  
Dna Sequence ◽  
Picea Glauca ◽  
Tandem Repeats ◽  
Picea Sitchensis ◽  
Repeated Dna ◽  
Metaphase Chromosomes ◽  
Genes Encoding ◽  
Repeated Dna Sequence

Repeated DNA families contribute to the large genomes of coniferous trees but are poorly characterized. We report the analysis of a 142 bp tandem repeated DNA sequence identified by the restriction enzyme Sau3A and found in approximately 20 000 copies in Picea glauca. Southern hybridization indicated that the repeated DNA family is specific to the genus, was amplified early in its evolution, and has undergone little structural alteration over evolutionary time. Fluorescence in situ hybridization localized arrays of the Sau3A repeating element to the centromeric regions of different subsets of the metaphase chromosomes of P. glauca and the closely related Picea sitchensis, suggesting that mechanisms leading to the intragenomic movement of arrays may be more active than those leading to mutation of the repeating elements themselves. Unambiguous identification of P. glauca and P. sitchensis chromosomes was made possible by co-localizing the Sau3A tandem repeats and the genes encoding the 5S and 18S-5.8S-26S ribosomal RNAs.Key words: Picea, repeated DNA, in situ hybridization, centromere.

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