Seroprevalence of Toxoplasma gondii and Neospora caninum in slaughtered pigs in the Czech Republic

Parasitology ◽  
2011 ◽  
Vol 138 (11) ◽  
pp. 1369-1371 ◽  
Author(s):  
E. BÁRTOVÁ ◽  
K. SEDLÁK
Keyword(s):  
Czech Republic ◽  
Toxoplasma Gondii ◽  
Immunosorbent Assay ◽  
Competitive Inhibition ◽  
Neospora Caninum ◽  
Natural Infection ◽  
Enzyme Linked Immunosorbent Assay ◽  
The Czech Republic ◽  
Specific Igg ◽  
Slaughtered Pigs

SUMMARYIn the Czech Republic, sera from 551 clinically healthy adult slaughtered pigs (females, 6–8 months old) were collected during the first half of June in 2010. Sera were tested for Toxoplasma gondii-specific IgG antibodies by an enzyme-linked immunosorbent assay; samples with more than 50% S/P were considered as positive. The same samples were also analysed for Neospora caninum antibodies using a commercial competitive-inhibition enzyme-linked immunosorbent assay; samples with more than 30% inhibition were considered as positive. Antibodies against T. gondii were found in 198 pigs (36%) in all districts with prevalences ranging from 18% to 75%. Antibodies against N. caninum were found in 16 pigs (3%); positive animals were found in 4 districts with prevalences ranging from 1% to 20%. Indication of mixed infections (concurrent presence of both N. caninum and T. gondii antibodies) was found in 8 (1·5%) pigs. The results of our study indicate that pigs in the Czech Republic have a relatively high seroprevalence for T. gondii, while they have only a low seroprevalence for N. caninum. Therefore, natural infection with T. gondii seems to be very common in Czech pigs. It is the first evidence of N. caninum antibodies in pigs in the Czech Republic. These results complete data about N. caninum infection in pigs in Europe.

scholarly journals  Toxoplasma gondii and Neospora caninum antibodies in goats in the Czech Republic

2012 ◽  
Vol 57 (No. 3) ◽  
pp. 111-114 ◽  
Author(s):  
E. Bartova ◽  
K. Sedlak
Keyword(s):  
Czech Republic ◽  
Toxoplasma Gondii ◽  
Immunosorbent Assay ◽  
Competitive Inhibition ◽  
Neospora Caninum ◽  
Fluorescent Antibody ◽  
Enzyme Linked Immunosorbent Assay ◽  
Serum Antibodies ◽  
Serum Samples ◽  
The Czech Republic

Toxoplasma gondii is zoonotic protozoan parasite that causes infections in many vertebrate species. The present study determined the seroprevalence of T. gondii and N. caninum in goats from the Czech Republic. Serum samples were collected from 251 healthy adult goats in the Czech Republic during the years 2006 to 2009. Sera samples were tested for serum antibodies to Toxoplasma gondii by an enzyme-linked immunosorbent assay with cut off equal to or higher than 50% S/P. The same samples were tested for serum antibodies to Neospora caninum by a competitive-inhibition enzyme-linked immunosorbent assay with cut off equal to or higher than 30% inhibition; positive sera were confirmed by an indirect fluorescent antibody test with cut-off titre equal to or higher than 40. Sera positive in both tests were marked as positive. In total, 166 (66%) and 15 (6%) goat sera reacted positively for T. gondii and N. caninum antibodies, respectively. All sera positive for N. caninum antibodies were simultaneously positive for T. gondii antibodies. This is the first detection of N. caninum antibodies in goats in the Czech Republic. Our findings indicate that goats in the Czech Republic are frequently exposed to T. gondii, but less frequently to N. caninum.  

Seroprevalence of Neospora caninum in cats from the Czech Republic

2014 ◽  
Vol 59 (2) ◽  
Author(s):  
Kamil Sedlák ◽  
Eva Bartova ◽  
Tereza Machacova
Keyword(s):  
Czech Republic ◽  
Competitive Inhibition ◽  
Neospora Caninum ◽  
Antibody Test ◽  
Enzyme Linked Immunosorbent Assay ◽  
Domestic Cats ◽  
The Czech Republic ◽  
Indirect Fluorescence Antibody ◽  
Different Parts ◽  
Sera Samples

AbstractSera of 414 cats coming from different parts of the Czech Republic were tested for N. caninum antibodies. Sera samples were collected during years 2002–2011. N. caninum antibodies were detected by a commercial competitive-inhibition enzyme-linked immunosorbent assay (cELISA) with cut off ≥30% inhibition. Samples positive in cELISA were confirmed by an indirect fluorescence antibody test (IFAT); titre ≥50 was considered positive. In total, 137 (33%) cats reacted positively in cELISA; N. caninum antibodies in IFAT were detected in 16 (3.86%) cats with titres 50 and 100. In 6 cats, positive for N. caninum antibodies, T. gondii antibodies were also detected by IFAT. It is the first report of N. caninum antibodies in domestic cats from the Czech Republic and third report in Europe.

scholarly journals Pestivirus infections in cervids from the Czech Republic

2009 ◽  
Vol 54 (No. 4) ◽  
pp. 191-193
Author(s):  
K. Sedlak ◽  
T. Girma ◽  
J. Holejsovsky
Keyword(s):  
Czech Republic ◽  
Cervus Elaphus ◽  
Red Deer ◽  
Competitive Inhibition ◽  
Enzyme Linked Immunosorbent Assay ◽  
Border Disease Virus ◽  
Serum Samples ◽  
The Czech Republic ◽  
Diarrhea Virus ◽  
Border Disease

372 sera of cervids from the Czech Republic were examined for antibodies to the bovine viral diarrhea virus (BVDV) and border disease virus (BDV) by competitive-inhibition enzyme-linked immunosorbent assay (ELISA), and for the presence of the BVDV by AgELISA. Antibodies to BVDV/BDV were found in 0.6% (two positive/305 tested) red deer (<I>Cervus elaphus</I>). BVDV/BDV antibodies were not found in four sika deer (<I>Cervus Nippon</I>) and 63 fallow deer (<I>Dama dama</I>). All serum samples were BVDV antigen negative. Our results confirmed that red deer in the Czech Republic are only rarely infected with Pestiviruses. This was the first survey of pestiviruses in farmed and wild cervids in the Czech Republic.

Survey of Toxoplasma gondii and Neospora caninum in raccoons (Procyon lotor) from the Czech Republic, Germany and Poland

2018 ◽  
Vol 262 ◽  
pp. 47-50 ◽  
Author(s):  
Aleksandra Kornacka ◽  
Aleksandra Cybulska ◽  
Marcin Popiołek ◽  
Natalia Kuśmierek ◽  
Bożena Moskwa
Keyword(s):  
Czech Republic ◽  
Toxoplasma Gondii ◽  
Procyon Lotor ◽  
Neospora Caninum ◽  
The Czech Republic

scholarly journals Detection of anti-avian bornavirus antibodies in parrots in the Czech Republic and Slovakia

2014 ◽  
Vol 83 (3) ◽  
pp. 195-199
Author(s):  
Martina Vondráčková ◽  
Viktor Tukač ◽  
Veronika Grymová ◽  
Pavlína Hájková ◽  
Zdeněk Knotek ◽  
...  
Keyword(s):  
Czech Republic ◽  
Immunosorbent Assay ◽  
Clinical Symptoms ◽  
Recent History ◽  
Enzyme Linked Immunosorbent Assay ◽  
Serum Samples ◽  
The Czech Republic ◽  
Avian Bornavirus ◽  
History Of ◽  
Proventricular Dilatation Disease

Since the avian bornavirus (ABV) aetiology of the proventricular dilatation disease (PDD) was proven in 2008, ABV has been detected in many avian species. The aim of the present study was to detect ABV antibodies in parrots in the Czech Republic and Slovakia. A total of 142 birds were examined, including 37 birds with symptoms typical for PDD, 54 birds without PDD symptoms, and 51 parrots without any clinical symptoms of PDD but originating from one flock with a proven history of PDD. Sera from 142 birds were tested using the enzyme-linked immunosorbent assay (ELISA) for detection of antibodies against ABV nucleoprotein p40. Of 142 serum samples, 71 were positive (50%) and 71 negative (50%). In a group of birds with clinical symptoms of PDD, 77.1% showed to be ABV positive, whereas in the group of sick birds without suspicion of PDD the percentage of positive birds was 31.6%. In the birds that had a cage mate that was positive for ABV or died with PDD, 42.9% were ABV positive. Of the parrots without PDD symptoms but originating from the flock with a recent history of PDD, 62.8% of the birds were positive for antibodies against ABV nucleoprotein p40. The results suggest that PDD is common and there is a high percentage of asymptomatic carriers of ABV in the breeding facilities of parrots in the Czech Republic and Slovakia.

Neospora caninum and Toxoplasma gondii antibodies in European brown hares in the Czech Republic, Slovakia and Austria

2010 ◽  
Vol 171 (1-2) ◽  
pp. 155-158 ◽  
Author(s):  
Eva Bártová ◽  
Kamil Sedlák ◽  
František Treml ◽  
Ivan Holko ◽  
Ivan Literák
Keyword(s):  
Czech Republic ◽  
Toxoplasma Gondii ◽  
Neospora Caninum ◽  
The Czech Republic
Sign in / Sign up

Export Citation Format

Share Document