scholarly journals Pestivirus infections in cervids from the Czech Republic

2009 ◽  
Vol 54 (No. 4) ◽  
pp. 191-193
Author(s):  
K. Sedlak ◽  
T. Girma ◽  
J. Holejsovsky
Keyword(s):  
Czech Republic ◽  
Cervus Elaphus ◽  
Red Deer ◽  
Competitive Inhibition ◽  
Enzyme Linked Immunosorbent Assay ◽  
Border Disease Virus ◽  
Serum Samples ◽  
The Czech Republic ◽  
Diarrhea Virus ◽  
Border Disease

372 sera of cervids from the Czech Republic were examined for antibodies to the bovine viral diarrhea virus (BVDV) and border disease virus (BDV) by competitive-inhibition enzyme-linked immunosorbent assay (ELISA), and for the presence of the BVDV by AgELISA. Antibodies to BVDV/BDV were found in 0.6% (two positive/305 tested) red deer (<I>Cervus elaphus</I>). BVDV/BDV antibodies were not found in four sika deer (<I>Cervus Nippon</I>) and 63 fallow deer (<I>Dama dama</I>). All serum samples were BVDV antigen negative. Our results confirmed that red deer in the Czech Republic are only rarely infected with Pestiviruses. This was the first survey of pestiviruses in farmed and wild cervids in the Czech Republic.

scholarly journals Pestivirus Infections in Semi-Domesticated Eurasian Tundra Reindeer (Rangifer tarandus tarandus): A Retrospective Cross-Sectional Serological Study in Finnmark County, Norway

Viruses ◽  
2019 ◽  
Vol 12 (1) ◽  
pp. 29 ◽  
Author(s):  
Carlos G. das Neves ◽  
Jonas Johansson Wensman ◽  
Ingebjørg Helena Nymo ◽  
Eystein Skjerve ◽  
Stefan Alenius ◽  
...  
Keyword(s):  
Rangifer Tarandus ◽  
Enzyme Linked Immunosorbent Assay ◽  
Border Disease Virus ◽  
Serum Samples ◽  
Cross Sectional ◽  
Diarrhea Virus ◽  
Border Disease ◽  
Serological Studies ◽  
Viral Diarrhea Virus

Members of the Pestivirus genus (family Flaviviridae) cause severe and economically important diseases in livestock. Serological studies have revealed the presence of pestiviruses in different cervid species, including wild and semi-domesticated Eurasian tundra reindeer. In this retrospective study, serum samples collected between 2006 and 2008 from 3339 semi-domesticated Eurasian reindeer from Finnmark County, Norway, were tested for anti-pestivirus antibodies using an enzyme linked immunosorbent assay (ELISA) and a subset of these by virus neutralization test (VNT). A seroprevalence of 12.5% was found, varying from 0% to 45% among different herding districts, and 20% in western Finnmark, as compared to 1.7% in eastern Finnmark. Seroprevalence increased with age. Pestivirus-specific RNA was not detected in any of the 225 serum samples tested by real-time RT-PCR. Based on VNT results, using a panel of one bovine viral diarrhea virus (BVDV) strain and two border disease virus (BDV) strains, the virus is most likely a reindeer-specific pestivirus closely related to BDV. A characterization of the causative virus and its pathogenic impact on reindeer populations, as well as its potential to infect other domestic and wild ruminants, should be further investigated.

scholarly journals  Toxoplasma gondii and Neospora caninum antibodies in goats in the Czech Republic

2012 ◽  
Vol 57 (No. 3) ◽  
pp. 111-114 ◽  
Author(s):  
E. Bartova ◽  
K. Sedlak
Keyword(s):  
Czech Republic ◽  
Toxoplasma Gondii ◽  
Immunosorbent Assay ◽  
Competitive Inhibition ◽  
Neospora Caninum ◽  
Fluorescent Antibody ◽  
Enzyme Linked Immunosorbent Assay ◽  
Serum Antibodies ◽  
Serum Samples ◽  
The Czech Republic

Toxoplasma gondii is zoonotic protozoan parasite that causes infections in many vertebrate species. The present study determined the seroprevalence of T. gondii and N. caninum in goats from the Czech Republic. Serum samples were collected from 251 healthy adult goats in the Czech Republic during the years 2006 to 2009. Sera samples were tested for serum antibodies to Toxoplasma gondii by an enzyme-linked immunosorbent assay with cut off equal to or higher than 50% S/P. The same samples were tested for serum antibodies to Neospora caninum by a competitive-inhibition enzyme-linked immunosorbent assay with cut off equal to or higher than 30% inhibition; positive sera were confirmed by an indirect fluorescent antibody test with cut-off titre equal to or higher than 40. Sera positive in both tests were marked as positive. In total, 166 (66%) and 15 (6%) goat sera reacted positively for T. gondii and N. caninum antibodies, respectively. All sera positive for N. caninum antibodies were simultaneously positive for T. gondii antibodies. This is the&nbsp;first detection of N.&nbsp;caninum antibodies in goats in the Czech Republic. Our findings indicate that goats in the Czech Republic are frequently exposed to T.&nbsp;gondii, but less frequently to N. caninum. &nbsp;

Seroprevalence of Neospora caninum in cats from the Czech Republic

2014 ◽  
Vol 59 (2) ◽  
Author(s):  
Kamil Sedlák ◽  
Eva Bartova ◽  
Tereza Machacova
Keyword(s):  
Czech Republic ◽  
Competitive Inhibition ◽  
Neospora Caninum ◽  
Antibody Test ◽  
Enzyme Linked Immunosorbent Assay ◽  
Domestic Cats ◽  
The Czech Republic ◽  
Indirect Fluorescence Antibody ◽  
Different Parts ◽  
Sera Samples

AbstractSera of 414 cats coming from different parts of the Czech Republic were tested for N. caninum antibodies. Sera samples were collected during years 2002–2011. N. caninum antibodies were detected by a commercial competitive-inhibition enzyme-linked immunosorbent assay (cELISA) with cut off ≥30% inhibition. Samples positive in cELISA were confirmed by an indirect fluorescence antibody test (IFAT); titre ≥50 was considered positive. In total, 137 (33%) cats reacted positively in cELISA; N. caninum antibodies in IFAT were detected in 16 (3.86%) cats with titres 50 and 100. In 6 cats, positive for N. caninum antibodies, T. gondii antibodies were also detected by IFAT. It is the first report of N. caninum antibodies in domestic cats from the Czech Republic and third report in Europe.

scholarly journals Serum neutralization as a differential serological test for classical swine fever virus and other pestivirus infections

1999 ◽  
Vol 51 (5) ◽  
pp. 403-408 ◽  
Author(s):  
J.C.M. Paredes ◽  
E.A.S. Oliveira ◽  
L.G. Oliveira ◽  
J.C.A. Rosa ◽  
P.M. Roehe
Keyword(s):  
Classical Swine Fever Virus ◽  
Serological Test ◽  
Sampling Strategy ◽  
Classical Swine Fever ◽  
Fever Virus ◽  
Border Disease Virus ◽  
Serum Samples ◽  
Diarrhea Virus ◽  
Serum Neutralization ◽  
Border Disease

Serum neutralization tests (SN) were performed against classical swine fever virus (CSFV), bovine viral diarrhea virus (BVDV) and border disease virus (BDV) on samples of swine serum collected for screening of antibodies to CSFV, in order to determine the SN value as a differential serological test. Ninety-nine sera out of a sample of 16,664 were positive for antibodies to pestiviruses in an ELISA test which did not distinguish antibodies to different pestiviruses. When submitted to SN, 81 sera were positive for CSFV antibodies only. In 17 sera, crossreactive antibodies to either CSFV, BVDV or BDV were detected. In most of these sera (13 out of 17) the differences between SN titres against the three viruses were not sufficient to estimate which was the most likely antibody-inducing virus. It was concluded that, for the SN to be useful in such differentiation, it is essential to examine a sample which must include a representative number of sera from the same farm where suspect animals were detected. When isolated serum samples are examined, such as those obtained with the sampling strategy adopted here, the SN may give rise to inconclusive results.

scholarly journals Analysis of accuracy of hunting plan in the Czech Republic

2012 ◽  
Vol 60 (3) ◽  
pp. 165-172 ◽  
Author(s):  
Radim Plhal ◽  
Jiří Kamler
Keyword(s):  
Czech Republic ◽  
Population Size ◽  
Cervus Elaphus ◽  
Calculation Method ◽  
Red Deer ◽  
Roe Deer ◽  
Capreolus Capreolus ◽  
Fallow Deer ◽  
Management Policy ◽  
The Czech Republic

This article analyses data on the reported and captured populations of red deer (Cervus elaphus), roe deer (Capreolus capreolus), fallow deer (Dama dama) and mouflon (Ovis musimon) in the Czech Republic during the years 2002–2006. Breeding and hunting plans collected by the State Hunting Authority have been summarized. The analyzed game population size (APS) for each individual territory, using the reverse calculation method, was compared to the counted population size (CPS) of each species. Next, the ratios between the specified population size (SPS) and the CPS were compared; and also the ratios of CPS and actual hunting bag were compared for individual years in the specified periods. Many errors in hunting management policy were found in all analyses regarding red deer. In many cases the APS, using the reverse calculation method, was several-times higher than the CPS. Also a large difference between SPS and CPS was very common. Similar results were also found in the analyses of fallow deer and mouflon populations, where the management policy was very similar to that of red deer. Evaluation of the roe deer management policy showed better results. Despite the fact that the APS was higher than the CPS, it was never several-times higher. Even in SPS and CPS ratios no extreme outcomes were found. From the official processed data it is evident that hunting association plans for game breeding and hunting indicate many significant problems, where the smallest problem is with roe deer and the biggest problem is with red deer.

scholarly journals Investigation of Bovine viral diarrhea virus (BVDV) and Border disease virus (BDV) infections of sheep in Southwestern Serbia

2010 ◽  
Vol 64 (5-6) ◽  
pp. 319-337
Author(s):  
Vladimir Kurcubic ◽  
Tamas Petrovic ◽  
Radojica Djokovic ◽  
Zoran Ilic
Keyword(s):  
Disease Virus ◽  
Bovine Viral Diarrhea Virus ◽  
Bovine Viral Diarrhea ◽  
Border Disease Virus ◽  
Serum Samples ◽  
Diarrhea Virus ◽  
Viral Diarrhea ◽  
Border Disease ◽  
Viral Diarrhea Virus ◽  
Experimental Group

Serological assay (virus neutralization test - VNT) was employed to examine blood sera collected from sheep of different age categories reared under different housing systems for the presence of bovine viral diarrhea virus (BVDV) and Border disease virus (BDV) infections in sheep. The objective of the investigation in sheep as reservoir hosts of the viruses was to clarify the potential for their transmission to cattle herds and vice versa. Experimental Group A included 5 mini farms composed entirely of sheep that were not in cohabitation with cattle in the same buildings or on the same pasture. Experimental Group B was made up of five mini sheep farms, whose owners raised cattle as well. A total of 10 blood samples (5 from junior categories of sheep aged up to 12 months and 5 from the older sheep) were secured from each of the 10 mini farms selected for the study. The VNT method did not detect the presence of specific anti-BVDV antibodies to both BVDV genotypes (BVDV-1 and BVDV-2) in any of the 100 test blood serum samples of sheep. Specific anti-BDV antibodies to the BDV Moredun strain were not found in any of the test serum samples of 100 sheep.

scholarly journals Development of a High-Throughput Serum Neutralization Test Using Recombinant Pestiviruses Possessing a Small Reporter Tag

Pathogens ◽  
2020 ◽  
Vol 9 (3) ◽  
pp. 188 ◽  
Author(s):  
Madoka Tetsuo ◽  
Keita Matsuno ◽  
Tomokazu Tamura ◽  
Takasuke Fukuhara ◽  
Taksoo Kim ◽  
...  
Keyword(s):  
High Throughput ◽  
Neutralization Test ◽  
Classical Swine Fever ◽  
Cross Reactivity ◽  
Enzyme Linked Immunosorbent Assay ◽  
Border Disease Virus ◽  
Diarrhea Virus ◽  
Serum Neutralization ◽  
Border Disease ◽  
Serum Neutralization Test

A serum neutralization test (SNT) is an essential method for the serological diagnosis of pestivirus infections, including classical swine fever, because of the cross reactivity of antibodies against pestiviruses and the non-quantitative properties of antibodies in an enzyme-linked immunosorbent assay. In conventional SNTs, an immunoperoxidase assay or observation of cytopathic effect after incubation for 3 to 7 days is needed to determine the SNT titer, which requires labor-intensive or time-consuming procedures. Therefore, a new SNT, based on the luciferase system and using classical swine fever virus, bovine viral diarrhea virus, and border disease virus possessing the 11-amino-acid subunit derived from NanoLuc luciferase was developed and evaluated; this approach enabled the rapid and easy determination of the SNT titer using a luminometer. In the new method, SNT titers can be determined tentatively at 2 days post-infection (dpi) and are comparable to those obtained by conventional SNTs at 3 or 4 dpi. In conclusion, the luciferase-based SNT can replace conventional SNTs as a high-throughput antibody test for pestivirus infections.

scholarly journals Detection of anti-avian bornavirus antibodies in parrots in the Czech Republic and Slovakia

2014 ◽  
Vol 83 (3) ◽  
pp. 195-199
Author(s):  
Martina Vondráčková ◽  
Viktor Tukač ◽  
Veronika Grymová ◽  
Pavlína Hájková ◽  
Zdeněk Knotek ◽  
...  
Keyword(s):  
Czech Republic ◽  
Immunosorbent Assay ◽  
Clinical Symptoms ◽  
Recent History ◽  
Enzyme Linked Immunosorbent Assay ◽  
Serum Samples ◽  
The Czech Republic ◽  
Avian Bornavirus ◽  
History Of ◽  
Proventricular Dilatation Disease

Since the avian bornavirus (ABV) aetiology of the proventricular dilatation disease (PDD) was proven in 2008, ABV has been detected in many avian species. The aim of the present study was to detect ABV antibodies in parrots in the Czech Republic and Slovakia. A total of 142 birds were examined, including 37 birds with symptoms typical for PDD, 54 birds without PDD symptoms, and 51 parrots without any clinical symptoms of PDD but originating from one flock with a proven history of PDD. Sera from 142 birds were tested using the enzyme-linked immunosorbent assay (ELISA) for detection of antibodies against ABV nucleoprotein p40. Of 142 serum samples, 71 were positive (50%) and 71 negative (50%). In a group of birds with clinical symptoms of PDD, 77.1% showed to be ABV positive, whereas in the group of sick birds without suspicion of PDD the percentage of positive birds was 31.6%. In the birds that had a cage mate that was positive for ABV or died with PDD, 42.9% were ABV positive. Of the parrots without PDD symptoms but originating from the flock with a recent history of PDD, 62.8% of the birds were positive for antibodies against ABV nucleoprotein p40. The results suggest that PDD is common and there is a high percentage of asymptomatic carriers of ABV in the breeding facilities of parrots in the Czech Republic and Slovakia.

Impact of allosucking on growth of farmed red deer calves (Cervus elaphus)

2001 ◽  
Vol 72 (3) ◽  
pp. 493-500 ◽  
Author(s):  
L. Bartoš ◽  
D. Vaňkovà ◽  
J. Hyànek ◽  
J. Šiler
Keyword(s):  
Body Weight ◽  
Czech Republic ◽  
Birth Weight ◽  
Cervus Elaphus ◽  
Feeding Behaviour ◽  
Red Deer ◽  
Nutritional Requirements ◽  
The Czech Republic ◽  
Relative Body Weight ◽  
South Bohemia

AbstractIt is generally presumed that allosucking brings benefits to the allosucking infants. Nevertheless, the data supporting such a presumption are rare. The aim of the study was to determine whether allosucking has any impact on growth rates of the allosucking calves. Fifty pregnant hinds were observed between 28 May (1st day of calving) and 2 September (abrupt weaning of all calves) on a red deer farm at Vimperk, South Bohemia, the Czech Republic. Of the 50 calves born the growth curve was calculated for 39 calves. During their 1st month of life these calves were observed in 1015 sucking bouts. In 690 cases the calves sucked from maternal hinds and in 325 cases non-maternal hinds. Only 25·64% of calves sucked exclusively from maternal hinds. The prevailing type of sucking behaviour was a combination of sucking from maternal hinds and allosucking (74·36%). Calves sucking from maternal and non-maternal hinds showed 1·6-fold higher sucking frequency than did calves feeding from maternal hinds only. Our results indicate that to some extend allonursing behaviour of the hind may affect their calves’ feeding behaviour. The more non-filial calves the maternal hind nursed, the higher frequency of the sucking by their calves occurred. The groups of calves did not differ in birth weight. With increasing age, the relative body weight increased faster in calves sucking maternal hinds only than in calves sucking maternal and non-maternal hinds. This gain in body weight was not essentially influenced by the fact whether or not the calf’s maternal hind nursed non-filial or exclusively filial calves. However, at weaning (99 days), the lowest body weight occurred in allosucking calves whose maternal hinds were allonursing. The results suggest that allosucking does not mean an extra profit to the allosucker. Instead, in our subjects, allosucking was rather attributed to compensation of nutritional requirements based on a combination of various factors, such as discrete differences in body weight at birth and also later and allonursing of the maternal hind.

scholarly journals Survey for pestiviruses in backyard pigs in southern Brazil

2020 ◽  
Vol 32 (1) ◽  
pp. 136-141 ◽  
Author(s):  
Ana C. S. Mósena ◽  
Matheus N. Weber ◽  
Samuel P. Cibulski ◽  
Mariana S. Silva ◽  
Willian P. Paim ◽  
...  
Keyword(s):  
Animal Health ◽  
Classical Swine Fever ◽  
Pcr Primers ◽  
Border Disease Virus ◽  
Southern Brazil ◽  
Serum Samples ◽  
Diarrhea Virus ◽  
Reverse Transcription Pcr ◽  
Border Disease ◽  
Two Samples

The Pestivirus genus comprises species that affect animal health and productivity worldwide. Members of the Suidae family are hosts for classical swine fever virus (CSFV), an important pathogen tracked by the World Organization for Animal Health (OIE). However, swine are also susceptible to other pestivirus species that can result in disease or compromise CSFV detection. We searched for pestivirus infection in swine sera collected from 320 backyard pig herds in southern Brazil. We used reverse-transcription PCR primers for Bungowannah virus; atypical porcine pestivirus (APPV); and a panpestivirus pair that detects bovine viral diarrhea virus (BVDV)-1, -2, and HoBi-like pestivirus (HoBiPeV), border disease virus (BDV), and CSFV. Two samples were positive using the panpestivirus primer pair and were classified as BVDV-1d and -2a, respectively. Serum samples were tested for virus neutralization against BVDV-1a, -1b, and -2 strains, resulting in 28 (4.4%) positive samples. Of those, 16 samples had the highest titers against BVDV-1a (2), BVDV-1b (5), and BVDV-2 (9). Our results indicate that Bungowannah virus, APPV, CSFV, BDV, and HoBiPeV have not been circulating in these specific backyard swine populations. However, ruminant pestiviruses were detected and must be considered in future pestivirus control programs conducted in Brazil.

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