Development of multiple-locus variable-number tandem-repeat analysis for rapid genotyping ofEhrlichia ruminantiumand its application to infectedAmblyomma variegatumcollected in heartwater endemic areas in Uganda

SUMMARYThe rickettsial bacteriumEhrlichia ruminantiumis the causative agent of heartwater, a serious tick-borne disease in ruminants. The genetic diversity of organisms in the field will have implications for cross-protective capacities of any vaccine developed, and for an effective vaccine design strategy proper genotyping and understanding of existing genetic diversity in the field is necessary. We searched for variable-number tandem-repeat (VNTR) loci for use in a multi-locus VNTR analysis (MLVA). Sequencing analysis of 30 potential VNTRs using a panel of 17 reference strains from geographically diverse origins identified 12 VNTRs with allelic profiles differing between strains. Application of MLVA to 38E. ruminantium-infectedAmblyomma variegatumcollected from indigenous cattle in 6 different districts of Uganda identified 21 MLVA types. The discriminatory power of MLVA was greater than that ofmap1PCR-restriction fragment length polymorphism analysis, with which only 6 genotypes were obtained. The high discriminatory power as well as cost- effective performance of MLVA provide the potential for this technique to be applied in the future with respect to optimizing vaccine trials by identifying local strain diversity, and also raise the possibility of exploring the association betweenE. ruminantiumgenotypes and phenotypes such as pathological outcome in the ruminant host.

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Mycobacterial Interspersed Repetitive Unit-variable Number Tandem Repeat (MIRU-VNTR) Typing Lacks Discriminatory Power in the Genetic Analysis of Bovine Tuberculosis in Egypt

American Journal of Microbiological Research ◽

10.12691/ajmr-5-6-5 ◽

2017 ◽

Vol 5 (6) ◽

pp. 142-147

Author(s):

Hebatallah Ahmed Mahgoub ◽

Mohamed F. Salama ◽

Mohamed El-Adl ◽

Ahmed Ammar ◽

Ali Amer ◽

...

Keyword(s):

Genetic Analysis ◽

Tandem Repeat ◽

Bovine Tuberculosis ◽

Variable Number Tandem Repeat ◽

Variable Number ◽

Discriminatory Power

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Variable Number Tandem Repeat (VNTR) analysis reveals genetic diversity within Mycoplasma mycoides mycoides Small Colony isolates from Nigeria

Veterinary Microbiology ◽

10.1016/j.vetmic.2010.05.020 ◽

2010 ◽

Vol 146 (3-4) ◽

pp. 354-355 ◽

Cited By ~ 2

Author(s):

N.D. Nwankpa ◽

L. Manso-silvan ◽

S. Lorenzon ◽

A. Yaya ◽

L.H. Lombin ◽

...

Keyword(s):

Genetic Diversity ◽

Tandem Repeat ◽

Variable Number Tandem Repeat ◽

Variable Number ◽

Mycoplasma Mycoides ◽

Small Colony ◽

Vntr Analysis

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Characterization of Genetic Diversity of Bacillus anthracis in France by Using High-Resolution Melting Assays and Multilocus Variable-Number Tandem-Repeat Analysis

Journal of Clinical Microbiology ◽

10.1128/jcm.05439-11 ◽

2011 ◽

Vol 49 (12) ◽

pp. 4286-4292 ◽

Cited By ~ 17

Author(s):

S. Derzelle ◽

S. Laroche ◽

P. Le Fleche ◽

Y. Hauck ◽

S. Thierry ◽

...

Keyword(s):

Genetic Diversity ◽

High Resolution ◽

Bacillus Anthracis ◽

Tandem Repeat ◽

High Resolution Melting ◽

Variable Number Tandem Repeat ◽

Variable Number ◽

Repeat Analysis

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A study of the variation in genetic diversity of Haemophilus Influenzae serotype b (Hib) strains in the UK between 1987 and 2010 using multilocus variable-number tandem repeat analysis (MLVA)

Journal of Infection ◽

10.1016/j.jinf.2011.04.169 ◽

2011 ◽

Vol 63 (6) ◽

pp. e100

Author(s):

Sapna Manglani ◽

David Litt ◽

Shona Neal ◽

Mary Slack

Keyword(s):

Genetic Diversity ◽

Haemophilus Influenzae ◽

Tandem Repeat ◽

Variable Number Tandem Repeat ◽

Variable Number ◽

Repeat Analysis ◽

Serotype B ◽

The Uk

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Multilocus variable number tandem repeat analysis (MLVA)-typing of Brucella abortus isolates of India reveals limited genetic diversity

Tropical Animal Health and Production ◽

10.1007/s11250-019-02110-x ◽

2019 ◽

Vol 52 (3) ◽

pp. 1187-1194

Author(s):

Maninder Singh ◽

Mohd Ashraf Malik ◽

Dhirender Kumar Singh ◽

Soni Doimari ◽

Bhavna ◽

...

Keyword(s):

Genetic Diversity ◽

Tandem Repeat ◽

Brucella Abortus ◽

Variable Number Tandem Repeat ◽

Variable Number ◽

Repeat Analysis ◽

Mlva Typing ◽

Limited Genetic Diversity

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Evaluation of the Epidemiological Relevance of Variable-Number Tandem-Repeat Genotyping of Mycobacterium bovis and Comparison of the Method with IS6110 Restriction Fragment Length Polymorphism Analysis and Spoligotyping

Journal of Clinical Microbiology ◽

10.1128/jcm.01349-06 ◽

2006 ◽

Vol 44 (9) ◽

pp. 3471-3471 ◽

Cited By ~ 2

Author(s):

C. Allix ◽

K. Walravens ◽

C. Saegerman ◽

J. Godfroid ◽

P. Supply ◽

...

Keyword(s):

Restriction Fragment Length Polymorphism ◽

Mycobacterium Bovis ◽

Length Polymorphism ◽

Tandem Repeat ◽

Fragment Length ◽

Fragment Length Polymorphism ◽

Variable Number Tandem Repeat ◽

Variable Number ◽

Polymorphism Analysis ◽

Restriction Fragment Length

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A new typing technique for the Rickettsiales Ehrlichia ruminantium: Multiple-locus variable number tandem repeat analysis

Journal of Microbiological Methods ◽

10.1016/j.mimet.2011.11.011 ◽

2012 ◽

Vol 88 (2) ◽

pp. 205-211 ◽

Cited By ~ 10

Author(s):

Héloïse Pilet ◽

Nathalie Vachiéry ◽

Moez Berrich ◽

Rim Bouchouicha ◽

Benoît Durand ◽

...

Keyword(s):

Tandem Repeat ◽

Variable Number Tandem Repeat ◽

Variable Number ◽

Ehrlichia Ruminantium ◽

Multiple Locus ◽

Repeat Analysis ◽

Typing Technique

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Genetic diversity of human isolates of Mycobacterium bovis assessed by spoligotyping and Variable Number Tandem Repeat genotyping

Infection Genetics and Evolution ◽

10.1016/j.meegid.2010.09.004 ◽

2011 ◽

Vol 11 (1) ◽

pp. 175-180 ◽

Cited By ~ 20

Author(s):

Nicoletta Lari ◽

Nicola Bimbi ◽

Laura Rindi ◽

Enrico Tortoli ◽

Carlo Garzelli

Keyword(s):

Genetic Diversity ◽

Mycobacterium Bovis ◽

Tandem Repeat ◽

Variable Number Tandem Repeat ◽

Variable Number

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Evaluation of the Epidemiological Relevance of Variable-Number Tandem-Repeat Genotyping of Mycobacterium bovis and Comparison of the Method with IS6110 Restriction Fragment Length Polymorphism Analysis and Spoligotyping

Journal of Clinical Microbiology ◽

10.1128/jcm.01775-05 ◽

2006 ◽

Vol 44 (6) ◽

pp. 1951-1962 ◽

Cited By ~ 80

Author(s):

C. Allix ◽

K. Walravens ◽

C. Saegerman ◽

J. Godfroid ◽

P. Supply ◽

...

Keyword(s):

Restriction Fragment Length Polymorphism ◽

Mycobacterium Bovis ◽

Length Polymorphism ◽

Tandem Repeat ◽

Fragment Length ◽

Fragment Length Polymorphism ◽

Variable Number Tandem Repeat ◽

Variable Number ◽

Polymorphism Analysis ◽

Restriction Fragment Length

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Development of a Multiple-Locus Variable-Number Tandem-Repeat Typing Scheme for Genetic Fingerprinting of Burkholderia cenocepacia and Application to Nationwide Epidemiological Analysis

Journal of Clinical Microbiology ◽

10.1128/jcm.02473-14 ◽

2014 ◽

Vol 53 (2) ◽

pp. 398-409 ◽

Cited By ~ 4

Author(s):

Christine Segonds ◽

Michelle Thouverez ◽

Antoine Barthe ◽

Nadège Bossuet-Greif ◽

Lenka Tisseyre ◽

...

Keyword(s):

Tandem Repeat ◽

Burkholderia Cepacia ◽

Genetic Relatedness ◽

Variable Number Tandem Repeat ◽

Cost Effective ◽

Variable Number ◽

Discriminatory Power ◽

Burkholderia Cenocepacia ◽

Content Type ◽

Burkholderia Multivorans

Organisms of theBurkholderia cepaciacomplex are especially important pathogens in cystic fibrosis (CF), with a propensity for patient-to-patient spread and long-term respiratory colonization.B. cenocepaciaandBurkholderia multivoransaccount for the majority of infections in CF, and major epidemic clones have been recognized throughout the world. The aim of the present study was to develop and evaluate a multilocus variable-number tandem-repeat (VNTR) analysis (MLVA) scheme forB. cenocepacia. Potential VNTR loci were identified upon analysis of the annotated genome sequences ofB. cenocepaciastrains AU1054, J2315, and MCO-3, and 10 of them were selected on the basis of polymorphisms and size. A collection of 100B. cenocepaciastrains, including epidemiologically related and unrelated strains, as well as representatives of the major epidemic lineages, was used to evaluate typeability, epidemiological concordance, and the discriminatory power of MLVA-10 compared with those of pulsed-field gel electrophoresis (PFGE) and multilocus sequence typing (MLST). Longitudinal stability was assessed by testing 39 successive isolates from 14 patients. Typeability ranged from 0.91 to 1, except for that of one marker, which was not amplified in 53% of theB. cenocepaciaIIIA strains. The MLVA types were shown to be stable in chronically colonized patients and within outbreak-related strains, with excellent epidemiological concordance. Epidemic and/or globally distributed lineages (epidemic Edinburgh-Toronto electrophoretic type 12 [ET-12], sequence type 32 [ST-32], ST-122, ST-234, and ST-241) were successfully identified. Conversely, the discriminatory power of MLVA was lower than that of PFGE or MLST, although PFGE variations within the epidemic lineages sometimes masked their genetic relatedness. In conclusion, MLVA represents a promising cost-effective first-line tool inB. cenocepaciasurveillance.

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