In vitro embryo production from bovine oocyte donors aspirated at different frequencies or following treatment with FSH

The combination of in vivo recovery of oocytes using transvaginal ultrasound guided aspiration and subsequent in vitro embryo production can be used to increase the rate of genetic change for efficiency of beef production by increasing selection intensity and reducing generation interval. The total number of oocytes recovered by aspiration and embryos produced is directly proportional to the number of aspiration sessions whether recovery takes place once or twice weekly. Pre-treatment of oocyte donors with FSH has been shown to improve the number of follicles available for aspiration but effects on embryo production have been conflicting (Bungartz et al., 1995; Goodhand et al., in press). The objective of this experiment was to compare the effect on embryo production of frequency of follicular aspiration and pre-treatment of donor cattle with FSH.

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In vivo oocyte recovery and in vitro embryo production from bovine oocyte donors treated with progestagen, oestradiol and FSH

Animal Reproduction Science ◽

10.1016/s0378-4320(00)00186-x ◽

2000 ◽

Vol 63 (3-4) ◽

pp. 145-158 ◽

Cited By ~ 30

Author(s):

K.L Goodhand ◽

M.E Staines ◽

J.S.M Hutchinson ◽

P.J Broadbent

Keyword(s):

Bovine Oocyte ◽

Embryo Production ◽

Oocyte Recovery ◽

In Vitro Embryo Production ◽

Oocyte Donors

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In vivo ultrasound guided transvaginal oocyte collection in the cow

Journal of the Hellenic Veterinary Medical Society ◽

10.12681/jhvms.15773 ◽

2018 ◽

Vol 49 (3) ◽

pp. 195

Author(s):

G. S. AMIRIDIS (Γ.Σ. ΑΜΟΙΡΙΔΗΣ) ◽

M. SALAHEDDINE ◽

I. A. JEFFCOATE ◽

E. VAINAS (Ε. ΒΑΪΝΑΣ) ◽

L. ROBERTSON

Keyword(s):

Recovery Rate ◽

Transvaginal Ultrasound ◽

Oestrous Cycle ◽

Ultrasound Guided ◽

Follicular Aspiration ◽

Oocyte Recovery ◽

Oocyte Collection ◽

Significant Difference

This paper describes the results of the in vivo ultrasound guided follicular aspiration for ovum pick υρ (OPU) in the cow. Twelve non pregnant dry cows aged 4-6 years were used in this experiment. Eight cows underwent OPU during three successive oestrous cycles and another four cows were used as controls having only transvaginal ultrasound scanning of their ovaries. Oocyte collection took place three times during the luteal phase of each natural oestrous cycle (days 3-4,9-11 and 14-17). The content of 326 follicles with a diameter of 4-15mm was aspirated and 104 oocytes were collected (recovery rate 31.9% or 1.55 oocytes per cow and session). The oocyte recovery rate increased after the first three sessions (from 13.04% to 35.0%) and reached levels of υρ to 52.6%. More follicles were aspirated on days 9-11 (133 follicles 40.8%) compared to 111 (34%) follicles on days 14-17 and 82 (25%) on days 3-4) (P<0.05). The evaluation of the collected oocytes revealed that 60 oocytes (57.7%) were suitable for further in vitro manipulation. Neither the origin of the oocyte (left or right ovary) nor the stage of the oestrous cycle affected the recovery rate or the quality of the collected oocytes. There was no significant difference either in the length of the oestrous cycle between the experimental animals and the controls (21.6± 1.4 vs. 22.37±1.0 respectively), or in plasma progesterone concentration in daily collected blood samples from the animals of the two groups. The results of this study are compared to those from the international literature and to the results from endoscopical methods for oocyte recovery. The feasibility of application of this technique to projects designed to improve the genetic merit of cows is discussed.

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In Vitro Embryo Production and Transfer of Bubaline Embryos using Oocytes Derived from Transvaginal Ultrasound-Guided Follicular Aspiration (TUFA)

International Journal of Applied Sciences and Biotechnology ◽

10.3126/ijasbt.v2i2.10369 ◽

2014 ◽

Vol 2 (2) ◽

pp. 180-184

Author(s):

FP Aquino ◽

Eufrocina P. Atabay

Keyword(s):

Transvaginal Ultrasound ◽

Blastocyst Stage ◽

Ultrasound Guided ◽

Blastocyst Development ◽

Embryo Production ◽

Follicular Aspiration ◽

Group 2 ◽

Group 1 ◽

Donor Cows

Transvaginal ultrasound-guided follicular aspiration (TUFA) has become a popular tool for embryo production in vitro due to its high degree of repeatability in terms of recovering oocytes from live animals. In Study 1, the quantity and quality of oocytes from Bulgarian Murrah buffalo cows (n=10) of varying ages (Group 1, 8-12; and Group 2, 13-17 years) were assessed. Group 1 buffalo donor cows yielded significantly higher (P<0.05) number of oocytes vs Group 2 buffalo donor cows (71 vs 29 oocytes, respectively), though in terms of oocyte quality, no difference was observed. In Study 2, oocytes collected (n=100) in Study 1 were matured, fertilized in vitro and the resulting zygotes were cultured which developed to blastocyst stage embryos. The maturation, fertilization and blastocyst development rates obtained were 53.0%, 40.0% and 32.5%, respectively. In Study 3, the viability of resulting blastocyst stage embryos was determined by transferring to recipient cows. Of 10 recipients 1 got pregnant and delivered a 35 kg male calf after 310 days gestation period. Overall, the results of the studies conducted demonstrated the potential of TUFA technology in the in vitro production of embryos which eventually could be used in the production of live offspring.DOI: http://dx.doi.org/10.3126/ijasbt.v2i2.10369Int J Appl Sci Biotechnol, Vol. 2(2): 180-184

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58 EFFICIENCY OF OVUM PICKUP AND EMBRYO PRODUCTION IN VITRO IN CLONED CATTLE

Reproduction Fertility and Development ◽

10.1071/rdv18n2ab58 ◽

2006 ◽

Vol 18 (2) ◽

pp. 137

Author(s):

A. Lucas-Hahn ◽

E. Lemme ◽

K.-G. Hadeler ◽

H.-G. Sander ◽

H. Niemann

Keyword(s):

Nuclear Transfer ◽

Transvaginal Ultrasound ◽

Statistical Significance ◽

Blastocyst Stage ◽

Ultrasound Guided ◽

Embryo Production ◽

Fetal Fibroblasts ◽

Developmental Rates ◽

In Vitro Embryo Production

The reproductive performance of cloned cattle was investigated by assessing the efficiency of transvaginal ultrasound-guided ovum pickup (OPU) and embryo production in vitro. Fetal fibroblasts from the endangered species, German Blackpied Cattle, had been used for nuclear transfer to produce three live cloned offspring (Lucas-Hahn et al. 2002 Theriogenology 57, 433). In the three cloned animals at 12–20 months of age, OPU was performed once per week and the total number of collected oocytes was recorded. In the case of Blondie, the procedure was terminated due to too small ovaries associated with insufficient function. Oocytes suitable for IVF were matured in vitro for 24 h and fertilized in vitro with the semen of a fertile bull. Oocytes derived from abbatoir ovaries were processed in parallel as controls. Embryos were in vitro-cultured in SOFaaBSA medium. Cleavage and developmental rates up to the morula/blastocyst stage were recorded in all groups. Statistical significance was tested using ANOVA and the Student-Newman-Keuls test. The results are presented in Table 1. Embryos from clones had lower cleavage and blastocyst rates compared to those derived from abattoir oocytes. However, results may have been confounded by potential OPU effects. Some of the blastocysts produced from Blacky (n = 5) and Paula (n = 2) were transferred to recipients. Two pregnancies resulted from the Paula transfers. The two male calves were delivered normally. After the completion of this experiment, all three cloned animals were artificially inseminated, became pregnant, delivered healthy calves, and are pregnant again at present. Further studies are needed to explore the fertility of cattle derived from somatic cloning. Table 1. OPU and in vitro embryo production in cloned cattle

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Transvaginal ultrasound-guided oocyte aspiration for production of embryos <i>in vitro</i>

Archives Animal Breeding ◽

10.5194/aab-45-99-2002 ◽

2002 ◽

Vol 45 (1) ◽

pp. 99-108

Author(s):

J. A. Carter ◽

S. Bellow ◽

M. Meintjes ◽

O. Perez ◽

E. Ferguson ◽

...

Keyword(s):

Transvaginal Ultrasound ◽

Farm Animals ◽

Embryo Research ◽

Ultrasound Guided ◽

Cyclic Activity ◽

Embryo Production ◽

Early Postpartum ◽

In Vitro Embryo Production ◽

Oocyte Aspiration

Abstract. reproductive potential in genetically valuable animals (BEAL et al., 1992). Now that repeatable oocyte retrieval methods are being fine-tuned, it is likely these procedures will become routinely used to obtain oocytes for further gamete and embryo research and also by seedstock producers for in vitro embryo production from farm animals in the commercial sector. The use of transvaginal ultrasound-guided oocyte aspiration and IVF procedure does offer an alternative to cattle producers who have genetically valuable cows that for some reason are unable to produce viable embryos through standard embryo collection procedures. This technology can be used on oocytes harvested from older ovulating or nonovulating cows, females with physical injuries (e.g., fractured leg) and problem cows having an abnormal cervix. Good success has been reported using IVF procedures on oocytes obtained from supplemental follicles of cows with cystic ovarian disease. With IVF the potential exists for more embryos to be produced in a shorter period of time, since the procedure can be repeated on the same cow 3 to 4 times or more a month. At this station, we are harvesting oocytes from early postpartum (< 40 days) beef and dairy cattle, before the female begins cyclic activity. The approach allows the opportunity to produce one or more extra calves from the cow before she is mated for a natural pregnancy. Currently, transvaginal ultrasound-guided oocyte aspiration is now being used to harvest valuable oocytes from minor farm animal breeds, from domestic females representing rare bloodlines, clinically infertile females and reproductively senescent cows. Research continues to find applications for this technology, including harvesting oocytes from young prepubertal heifers and early postpartum beef cows for in vitro embryo production. The use of ultrasound-guided oocyte aspiration should not be overlooked to obtain oocytes for in vitro embryo production and to aid in germplasm preservation of endangered exotic species.

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Transvaginal ultrasound-guided cumulus oocyte complexes aspiration and in vitro embryo production in suckled beef and lactating dairy cattle on pasture-based management conditions

Animal Reproduction Science ◽

10.1016/j.anireprosci.2011.10.001 ◽

2011 ◽

Vol 129 (1-2) ◽

pp. 1-6 ◽

Cited By ~ 8

Author(s):

M.H. Ratto ◽

O.A. Peralta ◽

G. Mogollon ◽

P. Strobel ◽

J. Correa

Keyword(s):

Dairy Cattle ◽

Transvaginal Ultrasound ◽

Ultrasound Guided ◽

Embryo Production ◽

In Vitro Embryo Production ◽

Lactating Dairy Cattle

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Pregnancy obtained in a late gestational mare by in vitro embryo production

Reproduction Fertility and Development ◽

10.1071/rd19305 ◽

2019 ◽

Vol 31 (12) ◽

pp. 1926

Author(s):

Lino Fernando Campos-Chillon ◽

Jan Martin ◽

Joy L. Altermatt

Keyword(s):

Transvaginal Ultrasound ◽

Experimental Studies ◽

Late Gestation ◽

Follicular Growth ◽

Ultrasound Guided ◽

Embryo Production ◽

In Vitro Embryo Production

Recently, the demand for invitro embryo production in the horse has increased worldwide. Most clinical transvaginal ultrasound-guided ovum pick-up (OPU) procedures are performed in non-pregnant donor mares, and few experimental studies have described invitro embryo production from oocytes of pregnant donors 21–150 days in gestation. This report discusses OPU, follicular growth and invitro embryo production in a pregnant mare during late gestation.

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