scholarly journals Hypervolemic-Hemodilution and Hypertension During Temporary Middle Cerebral Artery Occlusion in Rats: The Effect on Blood-Brain Barrier Permeability

1990 ◽  
Vol 17 (4) ◽  
pp. 372-377 ◽  
Author(s):  
Daniel J. Cole ◽  
John C. Drummond ◽  
Jerry S. Matsumura ◽  
Suzzanne Marcantonio ◽  
Bonnie I. Chi-Lum
Keyword(s):  
Blood Brain Barrier ◽  
Evans Blue ◽  
Artery Occlusion ◽  
Brain Barrier ◽  
Barrier Permeability ◽  
Blood Brain Barrier Permeability ◽  
Blood Brain ◽  
Brain Barrier Permeability ◽  
Hypervolemic Hemodilution ◽  
Cerebral Artery Occlusion

ABSTRACT:The effect of hypervolemic-hemodilution, with and without hypertension, on blood-brain barrier permeability was investigated in rats, after 180 minutes of middle cerebral artery occlusion (MCAo), and 60 minutes of reperfusion. One of the following conditions was maintained during MCAo: 1) Control — hematocrit and blood pressure were not manipulated; 2) Hypervolemic-Hemodilution/Normotension — the hematocrit was decreased to 30%; 3) Hypervolemic-Hemodilution/Hypertension — the hematocrit was decreased to 30% and mean arterial pressure increased by 30 mmHg with phenylphrine. In all groups, Evans Blue was administered, and its concentration determined by spectrophotometric assay. Evans Blue (μg{g-1 of brain tissue [mean ± SD]) was greater in the Hypervolemic- Hemodilution/Hypertension group (71 ± 20) versus the Control (13 ± 9) and Hypervolemic-Hemodilution/ Normotension (17 ± 10) groups (p < 0.05). No other differences were present. These results support the hypothesis that during MCAo, hypervolemic-hemodilution/hypertensive therapy effects an increase in blood-brain barrier permeability in the early period of reperfusion.

Measuring Blood-brain-barrier Permeability Using Evans Blue in Mice

BIO-PROTOCOL ◽  
2015 ◽  
Vol 5 (15) ◽  
Author(s):  
Jun-Xia Yang ◽  
Yan-Yu Jiang ◽  
Yu-Bai Guo
Keyword(s):  
Blood Brain Barrier ◽  
Evans Blue ◽  
Brain Barrier ◽  
Barrier Permeability ◽  
Blood Brain Barrier Permeability ◽  
Blood Brain ◽  
Brain Barrier Permeability

scholarly journals Gyroxin increases blood-brain barrier permeability to Evans blue dye in mice

Toxicon ◽  
2011 ◽  
Vol 57 (1) ◽  
pp. 162-167 ◽  
Author(s):  
J.A. Alves da Silva ◽  
K.C. Oliveira ◽  
M.A.P. Camillo
Keyword(s):  
Blood Brain Barrier ◽  
Evans Blue ◽  
Brain Barrier ◽  
Blue Dye ◽  
Barrier Permeability ◽  
Blood Brain Barrier Permeability ◽  
Evans Blue Dye ◽  
Blood Brain ◽  
Brain Barrier Permeability

Magnesium sulfate attenuates increased blood-brain barrier permeability during insulin-induced hypoglycemia in rats

2001 ◽  
Vol 79 (9) ◽  
pp. 793-798 ◽  
Author(s):  
Mehmet Kaya ◽  
Mutlu Küçük ◽  
Rivaze Bulut Kalayci ◽  
Vedat Cimen ◽  
Candan Gürses ◽  
...  
Keyword(s):  
Magnesium Sulfate ◽  
Blood Brain Barrier ◽  
Plasma Glucose ◽  
Evans Blue ◽  
Brain Barrier ◽  
Protective Effects ◽  
Barrier Permeability ◽  
Blood Brain Barrier Permeability ◽  
Blood Brain ◽  
Brain Barrier Permeability

Magnesium probably protects brain tissue against the effects of cerebral ischemia, brain injury and stroke through its actions as a calcium antagonist and inhibitor of excitatory amino acids. The effects of magnesium sulfate on cerebrovascular permeability to a dye, Evans blue, were studied during insulin-induced hypoglycemia with hypothermia in rats. Hypoglycemia was induced by an intramuscular injection of insulin. After giving insulin, each animal received MgSO4 (270 mg/kg) ip, followed by a 27 mg/kg dose every 20 min for 2.5 h. Plasma glucose and Mg2+ levels of animals were measured. Magnesium concentrations increased in the serum following MgSO4 administration (6.05 ± 0.57 vs. 2.58 ± 0.14 mg/dL in the Mg2+ group, and 7.14 ± 0.42 vs. 2.78 ± 0.06 mg/dL in the insulin + Mg2+ group, P < 0.01). Plasma glucose levels decreased following hypoglycemia (4 ± 0.66 vs. 118 ± 2.23 mg/dL in the insulin group, and 7 ± 1.59 vs. 118 ± 4.84 mg/dL in the insulin + Mg2+ group, P < 0.01). Blood-brain barrier permeability to Evans blue considerably increased in hypoglycemic rats (P < 0.01). In contrast, blood-brain barrier permeability to Evans blue was significantly reduced in treatment of hypoglycemic rats with MgSO4 (P < 0.01). These results indicate that Mg2+ greatly reduced the passage of exogenous vascular tracer bound to albumin into the brain during hypoglycemia with hypothermia. Mg2+ could have protective effects on blood-brain barrier permeability against insulin-induced hypoglycemia.Key words: blood-brain barrier, hypoglycemia, Mg2+, Evans-blue.

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