Diverse Effect of Microtuble Poisons to Alkaline Phosphatase Activity in Duodenum, Kidney, Liver and Serum of RAT and Mouse: A Histochemical Study

A single injection of colchicine(l-3mg/kg) has been reported to cause a remarkable induction of hepatic alkaline phosphatase(ALP ase) in rats.As an extension work of ALPase and calcium in duodenum we compared the degree of ALPase induction with colchicine and vinblastine in duodenum,kidney,liver and serum of rats and mice.Male Wister rats(B.W. 120-160g) and ICR mice(33-35g) were intravenously injected either colchicine(3mg/kg) or vinblastine(lmg/kg) after 5-6hr,10-12hr,or 20-22hr of the injection. ALPase was measured by Bessey-Lowry method using paranitrophenol phosphate as substrate. For electron microscopic histochemistry,tissue blocks were fixed in 2%glutaraldehyde for lhr and cut with Vibratome to be incubated in Mayahara's medium for 20min at 37°C.