Electron-microscopic cytochemistry of alkaline-phosphatase activity in endothelium, pericytes and oligodendrocytes in the rat brain

1985 ◽  
Vol 82 (3) ◽  
pp. 225-231 ◽  
Author(s):  
S. Mori ◽  
M. Nagano
Keyword(s):  
Alkaline Phosphatase ◽  
Rat Brain ◽  
Phosphatase Activity ◽  
Alkaline Phosphatase Activity ◽  
Electron Microscopic ◽  
Electron Microscopic Cytochemistry

Chlorpromazine and other psychoactive drug induced alterations of a membrane bound enzyme in rat brain

1994 ◽  
Vol 14 (3) ◽  
pp. 139-144
Author(s):  
Maitreyi Nag ◽  
Namita Nandi
Keyword(s):  
Alkaline Phosphatase ◽  
Rat Brain ◽  
Phosphatase Activity ◽  
Alkaline Phosphatase Activity ◽  
Drug Induced ◽  
Effective Inhibition ◽  
Membrane Bound ◽  
The Individual ◽  
Membrane Bound Enzymes ◽  
Combined Drugs

Psychoactive drugs like chlorpromazine (CPZ), imipramine, lithium and amphetamine in one way or another affect behaviour. The drug responses are presumably mediated by inducing a change in the activity of membrane bound enzymes. CPZ is very potent in inhibiting the alkaline phosphatase activity in rat brain. The combined effect of CPZ with other drugs shows that CPZ and imipramine together inhibit the enzyme activity significantly greater than the individual inhibition either by CPZ or by imipramine alone. Effective inhibition of the alkaline phosphatase activity with a single drug or combined drugs may lead to a change in neuronal permeability through glucocorticoids thereby affecting mood.

Diverse Effect of Microtuble Poisons to Alkaline Phosphatase Activity in Duodenum, Kidney, Liver and Serum of RAT and Mouse: A Histochemical Study

1982 ◽  
Vol 40 ◽  
pp. 38-39
Author(s):  
T. Makita ◽  
K. Sugiura ◽  
M. Hatsuoka
Keyword(s):  
Alkaline Phosphatase ◽  
Phosphatase Activity ◽  
Alkaline Phosphatase Activity ◽  
Histochemical Study ◽  
Single Injection ◽  
Electron Microscopic ◽  
Extension Work ◽  
Lowry Method ◽  
Rats And Mice ◽  
Kidney Liver

A single injection of colchicine(l-3mg/kg) has been reported to cause a remarkable induction of hepatic alkaline phosphatase(ALP ase) in rats.As an extension work of ALPase and calcium in duodenum we compared the degree of ALPase induction with colchicine and vinblastine in duodenum,kidney,liver and serum of rats and mice.Male Wister rats(B.W. 120-160g) and ICR mice(33-35g) were intravenously injected either colchicine(3mg/kg) or vinblastine(lmg/kg) after 5-6hr,10-12hr,or 20-22hr of the injection. ALPase was measured by Bessey-Lowry method using paranitrophenol phosphate as substrate. For electron microscopic histochemistry,tissue blocks were fixed in 2%glutaraldehyde for lhr and cut with Vibratome to be incubated in Mayahara's medium for 20min at 37°C.

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