Fine Structure of Meiotic Prophase Chromosomes in Lilium Longiflorum

1967 ◽  
Vol 25 ◽  
pp. 88-89
Author(s):  
Peter B. Moens
Keyword(s):  
Electron Microscopy ◽  
Drosophila Melanogaster ◽  
Meiotic Prophase ◽  
Lilium Longiflorum ◽  
Nurse Cells ◽  
Tomato Plants ◽  
Regional Conference ◽  
Mother Cells ◽  
European Regional ◽  
Dipteran Species

The presence of the tripartite ribbon within synapsed homologues has been reported for a large number of sexually reproducing organisms (over one hundred species, including fungi, plants, vertebrates and invertebrates). The absence of the ribbon in some species is associated with uncommon synaptic behaviour of meiotic prophase chromosomes (Drosophila melanogaster males, Drosophila melanogaster females homozygous for synapsis suppressing mutant C3G, and achiasmatic Dipteran species, reported by G. F. Meyer, 1964, Third European Regional Conference on Electron Microscopy). The tripartite ribbon, or synaptinemal complex, may therefore be assumed to be related to pairing of homologues at meiosis. The presence of the complexes and multi-complexes in non-meiotic cells such as insect obcyte nurse cells and spermatids suggests a somewhat broader function of the complexes. This is further supported by the occurrence of complexes in non-homologous paired chromosomes in the pollen mother cells of haploid tomato plants.

scholarly journals Proceedings of the European Regional Conference on Electron Microscopy, Delft 1960;1961

1962 ◽  
Vol 15 (10) ◽  
pp. 1063-1064
Author(s):  
V. E. Cosslett
Keyword(s):  
Electron Microscopy ◽  
Regional Conference ◽  
European Regional

scholarly journals DNA AND THE FINE STRUCTURE OF SYNAPTIC CHROMOSOMES IN THE DOMESTIC ROOSTER (GALLUS DOMESTICUS)

1964 ◽  
Vol 23 (1) ◽  
pp. 63-78 ◽  
Author(s):  
James R. Coleman ◽  
Montrose J. Moses
Keyword(s):  
Electron Microscopy ◽  
Heavy Metal ◽  
Fine Structure ◽  
Electron Microscope ◽  
Meiotic Prophase ◽  
Gallus Domesticus ◽  
Feulgen Staining ◽  
Synaptinemal Complex ◽  
Relationship Of ◽  
The Relationship

The indium trichloride method of Watson and Aldridge (38) for staining nucleic acids for electron microscopy was employed to study the relationship of DNA to the structure of the synaptinemal complex in meiotic prophase chromosomes of the domestic rooster. The selectivity of the method was demonstrated in untreated and DNase-digested testis material by comparing the distribution of indium staining in the electron microscope to Feulgen staining and ultraviolet absorption in thicker sections seen with the light microscope. Following staining by indium, DNA was found mainly in the microfibril component of the synaptinemal complex. When DNA was known to have been removed from aldehyde-fixed material by digestion with DNase, indium stainability was also lost. However, staining of the digested material with non-selective heavy metal techniques demonstrated the presence of material other than DNA in the microfibrils and showed that little alteration in appearance of the chromosome resulted from DNA removal. The two dense lateral axial elements of the synaptinemal complex, but not the central one to any extent, also contained DNA, together with non-DNA material.

European regional conference of the international geographical union

Geoforum ◽  
1972 ◽  
Vol 3 (1) ◽  
pp. 85-88
Author(s):  
Gy. Enyedi ◽  
S. Marosi
Keyword(s):  
Geographical Union ◽  
International Geographical ◽  
International Geographical Union ◽  
Regional Conference ◽  
European Regional

scholarly journals Basophilic Lamellar Systems in the Crayfish Spermatocyte

1958 ◽  
Vol 4 (3) ◽  
pp. 267-274 ◽  
Author(s):  
August Ruthmann
Keyword(s):  
Electron Microscopy ◽  
Small Rna ◽  
Meiotic Prophase ◽  
Lamellar Body ◽  
Annulate Lamellae ◽  
Late Prophase ◽  
Lamellar System ◽  
Thin Sections ◽  
Early Spermatid ◽  
Break Up

Histochemical procedures for the demonstration of RNA have shown the presence of intensely basophilic bodies in the cytoplasm of spermatocytes of the crayfish, Cambarus virilis. The staining of thick sections, cut alternately with thin sections for electron microscopy, has permitted identification of the basophilic bodies with two types of lamellar systems. One of these, a set of straight annulate lamellae, is restricted to meiotic prophase. The second type of lamellar systems has been found from late prophase to early spermatid stages. It consists of an ellipsoidal lamellar set which intersects a number of straight lamellae. Within the region of intersection, the ellipsoidal lamellae break up into an array of small tubules of about 150 A diameter. The term tubulate lamellar system was chosen to designate this type of lamellar complex. Small RNA-containing granules could not be detected in annulate lamellar systems. While there are a few granules in the marginal regions of the tubulate lamellar system, their distribution cannot be responsible for the basophilia which is intense within all regions of the lamellar body.

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