X-ray microanalytical and enzyme-cytochemical study on vesical malacoplakia

1978 ◽  
Vol 36 (2) ◽  
pp. 646-647
Author(s):  
Masami Hokano ◽  
Tsunao Oh-I ◽  
Yoshie Narita ◽  
Hiroshi Sassa ◽  
Saburo Suzuki
Keyword(s):  
Electron Microscopy ◽  
Electron Microscope ◽  
Osmium Tetroxide ◽  
Granulomatous Inflammation ◽  
Uranyl Acetate ◽  
The Other ◽  
Lead Citrate ◽  
Cytochemical Study ◽  
X Ray ◽  
Ultrathin Sections

Malacoplakia is a kind of granulomatous inflammation and characterized by the presence of calcium-stain positive granules (Michaelis-Gutmann bodies, hereinafter abbreviated as M-G bodies ) in the macrophages.In this report we want to say about the following articles:the ultrastruc tural findings in four cases of vesical malacoplakia;the ultrastructural morphogenesis of the M-G bodies;X-ray microanalytical studies which examine the change of the chemical component of M-G bodies, according to their developing stages; andacid phosphatase activity of lysosome within the malacoplakic macrophages.Biopsy materials taken from four cases with vesical malacoplakia were divided into 2 parts; one for a light microscopy and the other for an electron microscopy. The specimen for an electron microscopy was fixed in glutaraldehyde and osmium tetroxide, dehydrated in ethanol and then embedded in Epon 812. Ultrathin sections were doublestained with lead citrate and uranyl acetate and then subjected to routin TEM observation. For X-ray microanalysis was mounted an energy dispersive X-ray microanalyzer on a JEM-100 C type electron microscope. Ultrathin sections for microanalysis were cut 100-200nm thich and not stained.

Ultrastructure of Angiosarcoma of Mouse Tail

1980 ◽  
Vol 38 ◽  
pp. 740-741
Author(s):  
White Yvonne ◽  
Winslow Sheldon ◽  
James W. Townsend ◽  
Neil A. Littlefield
Keyword(s):  
Electron Microscopy ◽  
Electron Microscope ◽  
Light Microscopy ◽  
Toluidine Blue ◽  
Female Mouse ◽  
Osmium Tetroxide ◽  
Uranyl Acetate ◽  
Lead Citrate ◽  
Ultrathin Sections ◽  
Resin Mixture

Spontaneous neoplasms rarely occur on the tails of BALB/cStCrlfC3H/Nctr mice, but the neoplasm most frequently observed is a locally invasive non-metastatic angiosarcoma. In this case a female mouse weighing 33.2 g, 706 days of age, presented a soft, red, irregularly-shaped mass, measuring 18 mm in its greatest dimension, in the subcutis of the base of the tail. A portion of the tail tumor was taken for electron microscopy and the remainder was processed for light microscopy. The tissue processed for electron microscopy was fixed in 4% cacodyl ate-buffered glutaraldehyde, post-fixed in 1% osmium tetroxide, dehydrated in a graded series of ethanol solutions, and embedded in Epon-Araldite resin mixture. Sections of 1 μm were stained with toluidine blue for light microscopy and ultrathin sections of 100 nm were stained with uranyl acetate and lead citrate, then examined with a Philips EM201 electron microscope .

Electron microscopy of the changes in the sperm head curvature of the palm squirrel (Funambulus Penanti)

1993 ◽  
Vol 51 ◽  
pp. 334-335
Author(s):  
S. R. Bawa ◽  
R. Bawa ◽  
H. K. Bains
Keyword(s):  
Electron Microscopy ◽  
Plasma Membrane ◽  
Electron Microscope ◽  
Osmium Tetroxide ◽  
Uranyl Acetate ◽  
Sperm Head ◽  
Lead Citrate ◽  
Transmission Electron ◽  
Ultrathin Sections ◽  
Epididymal Spermatozoa

Examination of ultrathin sections of the spermatozoa recovered from the epididymis of the Indian palm squirrel (Funambulus penanti) indicates that the sperm head undergoes changes in its curvature during epididymal transit.Testis and epididymis of an adult male squirrel were dissected and small pieces of tissue fixed in 2.0% glutaraldehyde in 0.1 M phosphate buffer and post-fixed in osmium tetroxide. After dehydration in graded acetone the material was embedded in Araldite. Ultrathin sections were cut on a Reichert Jung Ultracut, picked-up on copper grids, stained with Reynold’s lead citrate-uranyl acetate and examined with a JEOL 1200 EX transmission electron microscope.Ultrathin sections of the caput epididymal spermatozoa reveal that their plasma membrane is adherent to the underlying acrosome (Figure 1). When these spermatozoa reach the corpus epididymis the plasma membrane surrounding the head becomes ruffled (Figure 2). The lifting-up of the plasma membrane around the head is restricted to the posterior bend of the acrosome.

Permeability in the Blood-Brain Barrier in Monkeys Following Exposure to High Altitude

1971 ◽  
Vol 29 ◽  
pp. 328-329
Author(s):  
R.S. Demaree ◽  
L.J. Ackerman ◽  
D. L. Anderson
Keyword(s):  
Electron Microscopy ◽  
Cerebral Edema ◽  
Osmium Tetroxide ◽  
Acute Mountain Sickness ◽  
Cebus Apella ◽  
Uranyl Acetate ◽  
Lead Citrate ◽  
Limited Evidence ◽  
Ultrathin Sections ◽  
Mountain Sickness

People who rapidly ascend to high terrestrial elevations may experience the “acute mountain sickness” syndrome. Speculation and limited evidence suggest that cerebral edema may play an important role in initiating and perpetuating this condition. We have recently demonstrated by electron microscopy that a mild cerebral edema develops in some Cebus apella monkeys rapidly transported to 14,110 feet. In the present study, Cebus apella monkeys were terminated at 1, 3, or 5 days after being shipped from sea level (160 feet) to 14,110 feet without acclimatization at intermediate altitudes.Thorotrast was administered IV 30 minutes prior to termination by perfusion or guillotine. Cerebral cortex was fixed by either perfusion or immersion in glutaraldehyde, and postfixed in osmium tetroxide. Following fixation, the tissues were dehydrated in ascending concentrations of ethanol followed by propylene oxide and embedded in Epon 812. Ultrathin sections were either not stained or doubly stained with uranyl acetate and lead citrate.

The Identification of Phospholipid Micelles in Glutaraldehyde-Urea Embedded Tissue

1975 ◽  
Vol 33 ◽  
pp. 494-495
Author(s):  
Daniel C. Pease
Keyword(s):  
Electron Micrographs ◽  
Lung Tissue ◽  
Osmium Tetroxide ◽  
Uranyl Acetate ◽  
Type Ii ◽  
Lead Citrate ◽  
Phospholipid Micelles ◽  
Type Ii Pneumocytes ◽  
Ultrathin Sections ◽  
Polar Water

It is reasonable to think that phospholipid micelles should be visible and identifiable in electron micrographs of ultrathin sections if only they can be preserved throughout the embedding process. The development of highly polar, water-containing, aminoplastic embedments has made this a likely possibility. With this in mind, an investigation of the lecithin-secreting, Type II pneumocytes of the lung is underway.Initially it has been easiest to recognize phospholipid micelles in lung tissue fixed first with glutaraldehyde, and then secondarily exposed to osmium tetroxide. However, the latter is not a necessary concomitant for micellar preservation. Conventional uranyl acetate and lead citrate staining is finally applied. Importantly, though, the micelles have been most easily seen in tissue embedded in 507. glutaraldehyde polymerized with urea, as described in detail by D.C. Pease and R.G. Peterson (J. Ultra- struct. Res., 41, 133, 1972). When oriented appropriately, the micellar units are seen as tiny, bilayer plates.

Ultrastructural Morphology of the Anagen Stage Hair Follicle of Male Beagle Dogs

1977 ◽  
Vol 35 ◽  
pp. 652-653
Author(s):  
F. A. Al-Bagdadi ◽  
C. W. Titkemeyer ◽  
J. E. Lovell
Keyword(s):  
Electron Microscope ◽  
Basement Membrane ◽  
Skin Biopsy ◽  
Osmium Tetroxide ◽  
Dermal Papilla ◽  
Uranyl Acetate ◽  
Ground Substance ◽  
Lead Citrate ◽  
Beagle Dogs ◽  
Cytoplasmic Processes

Skin biopsy samples were collected monthly from the lateral sides of 9 male Beagle dogs over a period of 1 year. The samples were fixed in 3% gluteral- dehyde and post fixed in 1% osmium tetroxide using phosphate or S-Collidine as buffers. They were dehydrated, embedded in Epon 812, sectioned with an LKB Ultrotome III, and stained with Reynolds' lead citrate and 1% uranyl acetate. They were examined and photographed by use of an RCA-3H electron microscope.The dermal papilla during anagen consisted of fibroblast-like cells some of which had cytoplasmic processes. The cytoplasm contained many mitochondria. (Fig. 1) The cells of the dermal papilla were either peripherally arranged spindle-shaped cells or polygonal cells. The basement membrane was either related to the fibroblast cytoplasmic processes or was free of the cytoplasmic processes but with a thick zone composed of fibers, granular ground substance and spaces.

Combined Plasma-Microincineration, Electron Microscopy And Electron Probe Microanalysis For Fine Localization of Biological Mineral Substances

1973 ◽  
Vol 31 ◽  
pp. 334-335 ◽  
Author(s):  
Richard S. Thomas ◽  
Mabel I. Corlett
Keyword(s):  
Electron Microscopy ◽  
Electron Microscope ◽  
Electron Probe ◽  
Oxygen Plasma ◽  
Chemical Nature ◽  
The Other ◽  
X Ray ◽  
Transmission Electron ◽  
Mineral Substances ◽  
Fine Localization

Ash patterns produced by oxygen plasma microincineration(OPM) of thin-sectioned biological materials and examined with the transmission electron microscope (TEM) can show unambiguously the distribution of mineral substances in the specimen with resolutions down to 100 Å. The chemical nature of the mineral is not demonstrated, however. Electron-probe X-ray microanalysis (EXM), on the other hand, can determine precisely the nature of the mineral in ashgd or unashed sections but its spatial resolution is limited to 1000-10,000 A at best. Also its sensitivity of analysis on unashed specimens is limited by intolerance of the specimen to high beam intensities. Using both TEM and EXM together on ash patterns of suitable specimens can overcome their independent spatial and chemical limitations. Furthermore, use of OPM produces a highly stable mineral specimen for EXM, thereby improving sensitivity.

Ultrahistochemical Analysis of the Chick Embryo Cornea Using A Non-Dispersive X-Ray Detector

1972 ◽  
Vol 30 ◽  
pp. 404-405
Author(s):  
T. Ohkura ◽  
M. Takashio ◽  
T. Watanabe
Keyword(s):  
Electron Microscopy ◽  
Chick Embryo ◽  
Alcian Blue ◽  
Uranyl Acetate ◽  
Lead Citrate ◽  
Thin Sections ◽  
X Ray ◽  
Transmission Electron ◽  
Glutaraldehyde Solution ◽  
Tissue Specimens

The experiments which we report here were performed to show qualitatively the presence or abscence of Cu in the mesostroma of the chick embryo cornea stained with alcian blue 8GS. Strips of the cornea were fixed in a buffered glutaraldehyde solution (2.5%, pH 7.4) and coloured with alcian blue 8GS at pH 2.5. Tissue specimens were postosmicated, dehydrated and embedded in Epon. Tissue blocks for the conventional transmission electron microscopy were prepared without alcian blue treatment, and the thin sections were stained with uranyl acetate and/or lead citrate. Fig. la shows the mesostroma of 3rd day chick embryo cornea; filaments run in various directions, and reveal no visible periodicity. Interfibrillar substances can not be demonstrated by the conventional method of the electron staining. The alcian blue treatment reveals the interfibrillar substances, which stand out in contrast as shown in Fig. lb.

Electron Microscopy of Wound Healing in Patients with Hernia

1975 ◽  
Vol 33 ◽  
pp. 352-353
Author(s):  
C.N. Sun ◽  
H.J. White ◽  
R.C. Read
Keyword(s):  
Electron Microscopy ◽  
Wound Healing ◽  
Phosphate Buffer ◽  
Osmium Tetroxide ◽  
Phosphotungstic Acid ◽  
Rectus Sheath ◽  
Uranyl Acetate ◽  
Collagen Fibrils ◽  
Lead Citrate ◽  
Native Collagen

Previously we have reported the defect of collagen fibrils from herniated rectus sheath. This presentation includes additional sections from postsurgical incisions (10 days) from both control and hernia patients. Small pieces of rectus sheath were fixed in 3% glutaraldehyde in phosphate buffer (pH 7.2) and post fixed with buffered 2% osmium tetroxide. The tissues were then dehydrated in serially increasing concentrations of alcohol and embedded in Epon 812. Sections were stained with 2.5% phosphotungstic acid or uranyl acetate and lead citrate.Previously we found that collagen fibrils from "non-herniated" rectus sheath have uniform diameters and 640 Å periodicity with seven or more intraperiodic bands resembling typical native collagen fibrils, while the fibrils from fascia obtained from patients with direct herniation show considerable variation in diameter. These variations are often found in the same individual fibers with a range from 300 Å to 3000 Å.

A Modified Technic of Staining Elastic Tissue for Electron Microscopic Study

1975 ◽  
Vol 33 ◽  
pp. 626-627
Author(s):  
J.A. Nordquist ◽  
K. Chrysant ◽  
A.K. Mandal
Keyword(s):  
Electron Microscopy ◽  
Osmium Tetroxide ◽  
Renal Tissue ◽  
Uranyl Acetate ◽  
Elastic Tissue ◽  
Lead Citrate ◽  
Electron Microscopic ◽  
Modified Method ◽  
Tetraphenyl Porphyrin ◽  
Normal Rat

By electron microscopy elastic tissue appear electrolucent in osmium fixed unstained grids as well as grids stained with uranyl acetate and lead citrate (UA + LC). Albert and Fleischer have studied aorta of mice with metalloporphyrins imparting conspicuous electron density to the elastic tissue. We are reporting here a modified method of electron microscopic (EM) study of the elastic tissue using metalloporhyrin, silver tetraphenyl porphyrin sulfonate (STPPS).We have studied the renal arterioles of rats and human in normal and diseased states. Elastic tissue of the aorta from young normal rat served as control for this study. Renal and aortic tissues were fixed in 4 percent glutaraldehyde, post fixed in 1 percent osmium tetroxide and embedded in spurr (blocks). From the blocks of renal tissue, 0.5 μ sections were cut, stained with methylene blue and azure II and studied by light microscopy.

scholarly journals Electron Microscopy of Retinal Photoreceptors

1960 ◽  
Vol 7 (3) ◽  
pp. 493-497 ◽  
Author(s):  
Arnaldo Lasansky ◽  
Eduardo de Robertis
Keyword(s):  
Electron Microscopy ◽  
Fine Structure ◽  
Electron Microscope ◽  
Outer Segment ◽  
Osmium Tetroxide ◽  
The Other ◽  
Rod Outer Segments ◽  
Outer Segments ◽  
Retinal Photoreceptors ◽  
L1 And L2

The fine structure of the cone and rod outer segments of the toad was studied under the electron microscope after fixation in osmium tetroxide and fixation in formaldehyde followed by chromation. In the OsO4-fixed specimens, the rod outer segment appears to be built of a stack of lobulated flattened sacs, each of which is made of two membranes of about 40 A separated by an innerspace of about 30 A. The distance between the rod sacs is about 50 A. The sacs in the cone outer segment are originated by the folding of a continuous membrane. The thickness of the membranes and width of the spaces between the cone sacs is the same as in rod, but the sac innerspace is slightly narrower in the cone (∼ 20 A). After fixation in formaldehyde and chromation, two different dense lines (l1 and l2) separated by spaces of less density appear. One of the lines, l1, has a thickness of 70 A and is less dense than the other, l2, which is 30 A thick. The correlation of the patterns obtained with both fixatives is considered and two possible interpretations are given. The possibility that l2 is related to a soluble phospholipid component is discussed. It is suggested that the outer segments have a paracrystallin organization similar to that found in myelin.

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