Techniques for preparing the Lolium perenne coleorhiza for scanning electron microscope evaluation

1992 ◽  
Vol 50 (1) ◽  
pp. 766-767
Author(s):  
Susan B.G. Debaene ◽  
John S. Gardner ◽  
Phil S. Allen
Keyword(s):  
Electron Microscope ◽  
Scanning Electron Microscope ◽  
Lolium Perenne ◽  
Morphological Study ◽  
Root Hairs ◽  
Inner Pressure ◽  
Water Potentials ◽  
Radicle Emergence ◽  
Standard Fixation ◽  
Scanning Electron

The coleorhiza is a nonvascular sheath that encloses the embryonic radicle in Poaceae, and is generally the first tissue to emerge during germination. Delicate hairlike extensions develop from some coleorhiza cells prior to radicle emergence. Similar to root hairs, coleorhiza hairs are extremely sensitive to desiccation and are damaged by exposure to negative water potentials. The coleorhiza of Lolium perenne is somewhat spherical when first visible, after which a knob forms at a right angle to the caryopsis due to inner pressure from the elongating radicle. This knob increases in length until the radicle finally punctures the coleorhiza. Standard fixation procedures cause severe desiccation of coleorhiza cells and hairs, making morphological study of the coleorhiza difficult. This study was conducted to determine a more successful process for coleorhiza preservation.

Chemical Reaction and Morphology of Polypropylene (PP)/Recycled Acrylonitrile Butadiene Rubber (NBRr)/Banana Skin Powder (BSP) Composites with γ-APS

2016 ◽  
Vol 1133 ◽  
pp. 175-179
Author(s):  
Azlinda Abdul Ghani ◽  
Ragunathan Santiagoo ◽  
Tunku Alisha Zanariah Tunku Ozir ◽  
Sam Sung Ting ◽  
Hanafi Ismail
Keyword(s):  
Surface Modification ◽  
Electron Microscope ◽  
Scanning Electron Microscope ◽  
Morphological Study ◽  
Functional Group ◽  
Butadiene Rubber ◽  
Intense Band ◽  
Acrylonitrile Butadiene Rubber ◽  
Roll Mill ◽  
Scanning Electron

Polypropylene (PP)/ recycled acrylonitrile butadiene rubber (NBRr)/ banana skin powder (BSP) composites were studied. Different BSP filer loading (5, 10, 15,20,25,30 wt. %) were prepared by using heated two roll mill at 180 °C. Then, the composites were tested for functional group using FTIR model Perkin Elmer Series 2. Field Emission Scanning Electron Microscope (VPFESEM) model Zeiss SUPRA 35VP also were using for morphological study. The effect of 3-aminopropyltrimethoxysilane (APS) as coupling agent were evaluated.The FTIR test shows different bands around 3200-3500 and 1740 cm-1 which represent the stretching of OH and C=O groups respectively. As for BSP which composed mostly of cellulose, hemicelluloses and lignin, the cellulose backbone C-OH is represent by the peaks of 1050 and 1048 cm-1 respectively. The γ-APS intense band around 1167 cm-1 and 1098 cm-1 in treated composite was assigned to the stretching of the-Si-O-Cellulose and –Si-O-Si-bond respectively. The large band around 1050 cm-1 found on BSP filler was attribute to the –Si-OH group which later this band will disappear after the surface modification. This evidenced that bonding between γ-APS treated BSP with PP/NBRr matrices. Morphological study supported this finding which BSP filler treated with γ-APS has improved the adhesion between BSP filler and PP/NBRr matrices.

Morphology and distribution of resting sporangia of Olpidium brassicae in tobacco roots

1976 ◽  
Vol 54 (24) ◽  
pp. 2820-2826 ◽  
Author(s):  
C. Hiruki ◽  
P. G. Alderson
Keyword(s):  
Electron Microscope ◽  
Scanning Electron Microscope ◽  
Vascular Tissue ◽  
Root Hairs ◽  
Epidermal Cells ◽  
Optical Section ◽  
Olpidium Brassicae ◽  
Tobacco Roots ◽  
Scanning Electron

The surface of resting sporangia, observed with the scanning electron microscope, was smooth and had well defined symmetry consisting of pentamer and hexamer facets. The number of facets was relative to the size of a resting sporangium. The resting sporangia formed regular ridges which appeared in optical section as stellar points. Resting sporangia were present in root hairs, epidermal cells, and cortex tissue but not in vascular tissue of infected roots.

A SERIAL SECTIONING TECHNIQUE FOR MORPHOLOGICAL STUDY OF COMPLEX CHITINOUS STRUCTURES WITH THE SCANNING ELECTRON MICROSCOPE

1977 ◽  
Vol 109 (3) ◽  
pp. 477-479
Author(s):  
J. J. B. Smith ◽  
W. G. Friend
Keyword(s):  
Electron Microscope ◽  
Scanning Electron Microscope ◽  
Morphological Study ◽  
Efficient Production ◽  
Serial Sections ◽  
Serial Sectioning ◽  
3 Dimensional ◽  
Internal Surfaces ◽  
Conventional Electron Microscopy ◽  
Scanning Electron

Special techniques are necessary to study the morphology of complex chitinous structures such as insect mouthparts. Many of the details are beyond the resolution of light microscopy, and the methods of conventional electron microscopy do not include the efficient production of serial sections for reconstruction. Although the scanning electron microscope (SEM) permits detailed observation of 3-dimensional surfaces, it cannot see internal surfaces and under complex folds, nor can it readily show the thickness of solid structures. To study the details of the stylet tips of the bug Rhodnius prolixus (Stål) several techniques were tried. Initially, specimens had been embedded in paraffin wax, sectioned, dewaxed, and prepared for the scanning electron microscope. Because of difficulties in sectioning the hard stylets and poor preservation of fine structure, this method was abandoned in favour of one using thick sections (2 μm) of material embedded in a mixture of Epon and Araldite.

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