Production and isozyme pattern of extracellular laccase in the S and P intersterility groups of the root pathogen Heterobasidion annosum

1999 ◽  
Vol 103 (3) ◽  
pp. 365-371 ◽  
Author(s):  
M. Johansson ◽  
M. Denekamp ◽  
F.O. Asiegbu
Keyword(s):  
Isozyme Pattern ◽  
Heterobasidion Annosum ◽  
Root Pathogen ◽  
Extracellular Laccase ◽  
Intersterility Groups

Relative abundance and potential dispersal range of intersterility groups of Heterobasidion annosum in pure and mixed forests

2001 ◽  
Vol 79 (9) ◽  
pp. 1057-1065 ◽  
Author(s):  
Paolo Gonthier ◽  
Matteo Garbelotto ◽  
Giovanna Cristina Varese ◽  
Giovanni Nicolotti
Keyword(s):  
Gene Flow ◽  
Host Specificity ◽  
Mixed Forest ◽  
Abies Alba ◽  
Heterobasidion Annosum ◽  
Silver Fir ◽  
Airborne Spores ◽  
Forest Pathogen ◽  
Limited Gene Flow ◽  
Intersterility Groups

In Europe the forest pathogen Heterobasidion annosum (Fr.) Bref. includes the S, P, and F intersterility groups (ISGs), each displaying a preferential specialization on Norway spruce (Picea abies (L.) Karst.), pine, and silver fir (Abies alba Mill.), respectively. In this paper, we present data about (i) H. annosum ISGs frequency in different forest types, (ii) the degree of host specificity of each ISG, (iii) the significance of the potential movement of airborne spores among forests, and (iv) the occurrence of S–P chimeras in the northwestern Alps. Using woody spore traps, we sampled natural pure spruce and fir forests and a mixed spruce-fir forest. The ISG of 582 spores was determined by ISG-diagnostic taxon-specific competitive priming (TSCP) polymerase chain reaction (PCR) combined with PCR-mediated detection of ISG-specific introns in the ML5–ML6 DNA region of the mitochondrial large ribosomal RNA (mt LrRNA). All three ISGs were found, and a strong correlation was observed between the F ISG and fir and the S ISG and spruce. In the mixed forest, no clear relationship between tree host species and host-specialized ISGs was found. In spite of a relative dominance of fir in the overstory of the mixed stand, the fir-associated F ISG represented only 11% of the total number of spores collected. This discrepancy was explained by the recent establishment of firs at this site. No S–P nuclear-mitochondrial chimeras were found. This suggests limited gene flow between these ISGs.Key words: Heterobasidion annosum, host specificity, ISGs, gene flow, PCR, Alps.

Intersterility groups of Heterobasidion annosum in some spruce and pine stands in Byelorussia, Lithuania and Estonia

1992 ◽  
Vol 22 (6-7) ◽  
pp. 384-391 ◽  
Author(s):  
K. Korhonen ◽  
I. Bobko ◽  
S. Hanso ◽  
T. Piri ◽  
A. Vasiliauskas
Keyword(s):  
Heterobasidion Annosum ◽  
Pine Stands ◽  
Intersterility Groups

Population structure of Heterobasidion annosum from North America and Europe

1993 ◽  
Vol 71 (8) ◽  
pp. 1064-1071 ◽  
Author(s):  
William J. Otrosina ◽  
Thomas E. Chase ◽  
Fields W. Cobb Jr. ◽  
Kari Korhonen
Keyword(s):  
Population Structure ◽  
North America ◽  
North American ◽  
Forest Tree ◽  
Heterobasidion Annosum ◽  
Evolutionary Relationships ◽  
The North ◽  
Isozyme Loci ◽  
Intersterility Groups ◽  
European Populations

Isolates of Heterobasidion annosum (Fr.) Bref. representing North American S and P and European S, P, and F intersterility groups were subjected to isozyme analysis. European S, P, and F groups had more variability than the North American S and P groups in expected hterozygosity, number of alleles per locus, and percent polymorphic loci. In contrast with the North American S and P groups, the European intersterility groups could not be distinguished from each other on the basis of individual isozyme loci, although significant differences in allele frequencies exist between European S and P groups. This suggests that evolution proceeded at different rates in the intersterility groups, or intersterility barriers appeared later in the European populations relative to the North American populations of H. annosum. Changes in climate and host species associations during the Tertiary may have been a major factor in evolution of H. annosum intersterility groups. Key words: allozymes, forest tree hosts, playnological events, evolutionary relationships, Hymenomycetes, root disease.

Combined fatty acid and sterol profiles of Heterobasidion annosum intersterility groups S, P and F

1995 ◽  
Vol 99 (9) ◽  
pp. 1025-1033 ◽  
Author(s):  
M.M. Müller ◽  
R. Kantola ◽  
K. Korhonen ◽  
J. Uotila
Keyword(s):  
Fatty Acid ◽  
Heterobasidion Annosum ◽  
Intersterility Groups

Differentiation of intersterility groups and geographic provenances among isolates of Heterobasidion annosum detected by random amplified polymorphic DNA assays

1993 ◽  
Vol 71 (4) ◽  
pp. 565-569 ◽  
Author(s):  
Matteo Garbelotto ◽  
Thomas D. Bruns ◽  
Fields W. Cobb ◽  
William J. Otrosina
Keyword(s):  
North American ◽  
Random Amplified Polymorphic Dna ◽  
Heterobasidion Annosum ◽  
Isozyme Electrophoresis ◽  
Length Polymorphisms ◽  
Dna Assays ◽  
Herbarium Collection ◽  
Polymerase Chain ◽  
Successful Amplification ◽  
Intersterility Groups

Random amplified polymorphic DNAs were correlated with the intersterility group and the geographic provenance of 36 isolates of Heterobasidion annosum from North America and Europe and of one herbarium collection of basidiocarps from California. This technique is very precise and yields higher resolution than previous studies implementing techniques such as isozyme electrophoresis and restriction fragment length polymorphisms. Random amplified polymorphic DNAs revealed differentiation among the following geographic groups and intersterility groups: western North American P, eastern North American P, European P, North American S, Scandinavian S, Italian S, and Italian F. This is the first report on differentiation between eastern and western North American P isolates as well as between northern and southern European S isolates. Successful amplification of one dry basidiocarp suggests that random amplified polymorphic DNAs may be used to improve epidemiological and population studies of this pathogen. Key words: species complex, genetic variability, strain typing, forest pathology, polymerase chain reaction.

scholarly journals The influence of Hypholoma fasciculare and Phlebiopsis gigantea on the growth of Heterobasidion annosum in vitro

2014 ◽  
Vol 33 (1) ◽  
pp. 147-154
Author(s):  
Piotr Łakomy ◽  
Joanna Zieniewicz ◽  
Tomasz Świdkiewicz
Keyword(s):  
Heterobasidion Annosum ◽  
Root Pathogen ◽  
Phlebiopsis Gigantea ◽  
Pathogen Strains ◽  
Hypholoma Fasciculare

The influence of two saprotrophes — isolates of <i>Hypholoma fasciculare</i> and <i>Phlebiopsis gigantea</i> on the growth of thirty three root pathogen strains — <i>Heterobasidion annosum</i> was analysed. Two methods were used. The different reaction in paired cultures among saprotrophe and pathogen isolates suggest, that one isolate of <i>H. annosum</i> is not enough to study the interaction between this pathogen and saprophytes in vitro irrespective of the method used.

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