Photothermal quantitation of nanogram quantities of Coomassie Brilliant Blue stained proteins in denaturing polyacrylamide gels

1986 ◽  
Vol 58 (13) ◽  
pp. 2876-2879 ◽  
Author(s):  
Konan. Peck ◽  
Michael D. Morris
Keyword(s):  
Brilliant Blue ◽  
Polyacrylamide Gels ◽  
Coomassie Brilliant Blue ◽  
Coomassie Brilliant

scholarly journals Elution of proteins from polyacrylamide eels: a simple and economic procedure

1989 ◽  
Vol 31 (1) ◽  
pp. 44-47
Author(s):  
Vera Bongertz
Keyword(s):  
Gel Electrophoresis ◽  
Brilliant Blue ◽  
Polyacrylamide Gels ◽  
Coomassie Brilliant Blue ◽  
Coomassie Brilliant

A simplified methodology for the quantitative electroelution of proteins from polyacrylamide gels is described. After staining with Coomassie Brilliant Blue R 250, the identified bands are excised from the gel and the proteins eluted using a procedure developed for use in conventional tube gel electrophoresis equipment.

Destaining of Coomassie Brilliant Blue R-250-stained polyacrylamide gels with fungal laccase

2016 ◽  
Vol 493 ◽  
pp. 27-29 ◽  
Author(s):  
Jie Yang ◽  
Xiaodan Yang ◽  
Xiuyun Ye ◽  
Juan Lin
Keyword(s):  
Brilliant Blue ◽  
Polyacrylamide Gels ◽  
Coomassie Brilliant Blue ◽  
Fungal Laccase ◽  
Coomassie Brilliant

Coomassie-Brilliant Blue Staining of Polyacrylamide Gels

2012 ◽  
pp. 465-469 ◽  
Author(s):  
Claudia Arndt ◽  
Stefanie Koristka ◽  
Anja Feldmann ◽  
Holger Bartsch ◽  
Michael Bachmann
Keyword(s):  
Brilliant Blue ◽  
Blue Staining ◽  
Polyacrylamide Gels ◽  
Coomassie Brilliant Blue ◽  
Coomassie Brilliant Blue Staining ◽  
Coomassie Brilliant

scholarly journals Effect of pre-stain methanol washing on the sensitivity of CBBR-250 staining in SDS-polyacrylamide gels

2015 ◽  
Vol 2 (1) ◽  
Author(s):  
Rashid Saleem ◽  
Absar-ul Hasnain ◽  
Riaz Ahmad
Keyword(s):  
Molecular Weight ◽  
Acetic Acid ◽  
Staining Method ◽  
Muscle Protein ◽  
Brilliant Blue ◽  
Low Molecular Weight ◽  
Polyacrylamide Gels ◽  
Coomassie Brilliant Blue ◽  
Protein Research ◽  
Coomassie Brilliant

AbstractCoomassie Brilliant Blue R-250 (CBBR-250) is the most frequently used dye stain in protein research. However, relatively poor sensitivity and long application durations are the major limitations of this stain. In the present study, we have investigated the effect of pre-stain methanol washing on the sensitivity of the conventional CBB staining method. Concentrations of methanol ranging from 5 to 30% were prepared in 5% acetic acid. Pre-stain washing of SDS-gels using acetic acid (5%) and methanol were assessed at various timings and temperatures. Our results demonstrate that pre-stain washing by methanol greatly improves the sensitivity of the CBB stain and facilitates the visualization of low molecular weight components present in a complex muscle protein, actomyosin. The outcome of this study also revealed duration of pre-stain washing and temperature as other factors that determine the sensitivity of CBB staining method. Therefore, it is suggested that pre-stain washing with methanol may improve the sensitivity of CBB stain in SDS-PA gels.

Sign in / Sign up

Export Citation Format

Share Document