Investigations of stationary phase modification by the mobile phase surfactant in micellar liquid chromatography

1989 ◽  
Vol 61 (13) ◽  
pp. 1353-1358 ◽  
Author(s):  
Michael F. Borgerding ◽  
Willie L. Hinze ◽  
Larry D. Stafford ◽  
George W. Fulp ◽  
William C. Hamlin
Keyword(s):  
Liquid Chromatography ◽  
Stationary Phase ◽  
Mobile Phase ◽  
Micellar Liquid Chromatography ◽  
Phase Modification

Solute–mobile phase and solute–stationary phase interactions in micellar liquid chromatography. A review

The Analyst ◽  
1992 ◽  
Vol 117 (5) ◽  
pp. 831-837 ◽  
Author(s):  
María José Medina Hernández ◽  
María Celia García Alvarez-coque
Keyword(s):  
Liquid Chromatography ◽  
Stationary Phase ◽  
Mobile Phase ◽  
Micellar Liquid Chromatography

scholarly journals Analysis of Some Biogenic Amines by Micellar Liquid Chromatography

2012 ◽  
Vol 2012 ◽  
pp. 1-8 ◽  
Author(s):  
Irena Malinowska ◽  
Katarzyna E. Stępnik
Keyword(s):  
Liquid Chromatography ◽  
Sodium Dodecyl Sulfate ◽  
Biogenic Amines ◽  
Physicochemical Parameters ◽  
Mobile Phase ◽  
Sodium Dodecyl ◽  
Micellar Liquid Chromatography ◽  
Ionic Surfactant ◽  
Mobile Phases ◽  
Dodecyl Sulfate

Micellar liquid chromatography (MLC) with the use of high performance liquid chromatography (HPLC) was used to determine some physicochemical parameters of six biogenic amines: adrenaline, dopamine, octopamine, histamine, 2-phenylethylamine, and tyramine. In this paper, an influence of surfactant’s concentration and pH of the micellar mobile phase on the retention of the tested substances was examined. To determine the influence of surfactant’s concentration on the retention of the tested amines, buffered solutions (at pH 7.4) of ionic surfactant—sodium dodecyl sulfate SDS (at different concentrations) with acetonitrile as an organic modifier (0.8/0.2 v/v) were used as the micellar mobile phases. To determine the influence of pH of the micellar mobile phase on the retention, mobile phases contained buffered solutions (at different pH values) of sodium dodecyl sulfate SDS (at 0.1 M) with acetonitrile (0.8/0.2 v/v). The inverse of value of retention factor () versus concentration of micelles () relationships were examined. Other physicochemical parameters of solutes such as an association constant analyte—micelle ()—and partition coefficient of analyte between stationary phase and water (hydrophobicity descriptor) () were determined by the use of Foley’s equation.

scholarly journals LC and UV Methods for Lamotrigine Determination in Pharmaceutical Formulation

2011 ◽  
Vol 2011 ◽  
pp. 1-8 ◽  
Author(s):  
Magda T. Martins ◽  
Clésio S. Paim ◽  
Martin Steppe
Keyword(s):  
Liquid Chromatography ◽  
Stationary Phase ◽  
Mobile Phase ◽  
Pharmaceutical Formulation ◽  
Stress Conditions ◽  
Phase Detection ◽  
Photodiode Array ◽  
Detection Response ◽  
Student Test ◽  
Interday Precision

Liquid chromatography (LC) and ultraviolet spectrophotometric (UV) methods for lamotrigine (LTG) determination were validated. The LC separation was achieved on an ACE RP-18 as stationary phase and 0.3% triethylamine in water (v/v) pH 4.0 : methanol (62 : 38, v/v) as mobile phase. Detection was achieved with a photodiode array at 279 nm. The detection response for LTG was linear (). The specificity and stability were proved using stress conditions. The CV (%) values for intraday and interday precision were less than 2.0%. The method was accurate and robust. The -student test proved that the LC and UV methods are interchangeable.

Simultaneous Determination of Floctafenine and its Hydrolytic Degradation Product Floctafenic Acid Using Micellar Liquid Chromatography with Applications to Tablets and Human Plasma

2013 ◽  
Vol 96 (6) ◽  
pp. 1315-1324 ◽  
Author(s):  
Mohamed I Walash ◽  
Fathalla Belal ◽  
Nahed El-Enany ◽  
Manal Eid ◽  
Rania N El-Shaheny
Keyword(s):  
Liquid Chromatography ◽  
Human Plasma ◽  
Degradation Product ◽  
Mobile Phase ◽  
Sodium Dodecyl ◽  
Hydrolytic Degradation ◽  
Direct Determination ◽  
Micellar Liquid Chromatography ◽  
Main Metabolite

Abstract A stability-indicating micellar liquid chromatography (MLC) method was developed and validated for the assay of floctafenine (FLF) in the presence of its degradation product and main metabolite, floctafenic acid (FLA). The analysis was carried out on a CLC Shim-Pack octyl silane (C8) column (150 × 4.6 mm id, 5 μm particle size) using a micellar mobile phase consisting of 0.15 M sodium dodecyl sulfate, 10% n-propanol, and 0.3% triethylamine in 0.02 M orthophosphoric acid (pH = 3). The mobile phase was pumped at a flow rate of 1.0 mL/min with UV detection at 360 nm. The method showed good linearity for FLF and FLA over the concentration ranges of 0.5–25.0 and 0.4–10.0 μg/mL, with LODs of 0.16 and 0.12 μg/mL, respectively. The developed method was successfully applied to the determination of FLF in commercial dispersible tablets, with mean recovery of 98.87 ± 1.37%. Also, the proposed method was specific for the analysis of FLF in presence of the co-formulated drug thiocolchicoside in laboratory-prepared tablets, with mean recovery of 100.50 ± 1.07%. Statistical comparison of the results obtained by the proposed MLC method with those obtained by a comparison method showed good agreement. Moreover, the method was extended to study the degradation behavior of FLF under different International Conference on Harmonization recommended conditions such as alkaline, acidic, oxidative, thermal, and photolytic. The method was further applied for direct determination of FLA as the main metabolite of FLF in human plasma without prior extraction steps, with mean recovery of 110.50 ± 6.5%.

Calorimetry and liquid chromatography: determination of enthalpies of transfer from mobile phase to stationary phase

2004 ◽  
Vol 424 (1-2) ◽  
pp. 157-163 ◽  
Author(s):  
Koji Urakami ◽  
Ken Hosoya ◽  
Anthony E. Beezer
Keyword(s):  
Liquid Chromatography ◽  
Stationary Phase ◽  
Mobile Phase ◽  
Enthalpies Of Transfer

A model describing the effect on retention of the addition of alcohols to the mobile phase in micellar liquid chromatography

1996 ◽  
Vol 719 (1) ◽  
pp. 15-26 ◽  
Author(s):  
O. Jiménez ◽  
M.A. García ◽  
M.L. Marina
Keyword(s):  
Liquid Chromatography ◽  
Mobile Phase ◽  
Micellar Liquid Chromatography
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