scholarly journals LC and UV Methods for Lamotrigine Determination in Pharmaceutical Formulation

2011 ◽  
Vol 2011 ◽  
pp. 1-8 ◽  
Author(s):  
Magda T. Martins ◽  
Clésio S. Paim ◽  
Martin Steppe
Keyword(s):  
Liquid Chromatography ◽  
Stationary Phase ◽  
Mobile Phase ◽  
Pharmaceutical Formulation ◽  
Stress Conditions ◽  
Phase Detection ◽  
Photodiode Array ◽  
Detection Response ◽  
Student Test ◽  
Interday Precision

Liquid chromatography (LC) and ultraviolet spectrophotometric (UV) methods for lamotrigine (LTG) determination were validated. The LC separation was achieved on an ACE RP-18 as stationary phase and 0.3% triethylamine in water (v/v) pH 4.0 : methanol (62 : 38, v/v) as mobile phase. Detection was achieved with a photodiode array at 279 nm. The detection response for LTG was linear (). The specificity and stability were proved using stress conditions. The CV (%) values for intraday and interday precision were less than 2.0%. The method was accurate and robust. The -student test proved that the LC and UV methods are interchangeable.

Investigations of stationary phase modification by the mobile phase surfactant in micellar liquid chromatography

1989 ◽  
Vol 61 (13) ◽  
pp. 1353-1358 ◽  
Author(s):  
Michael F. Borgerding ◽  
Willie L. Hinze ◽  
Larry D. Stafford ◽  
George W. Fulp ◽  
William C. Hamlin
Keyword(s):  
Liquid Chromatography ◽  
Stationary Phase ◽  
Mobile Phase ◽  
Micellar Liquid Chromatography ◽  
Phase Modification

Calorimetry and liquid chromatography: determination of enthalpies of transfer from mobile phase to stationary phase

2004 ◽  
Vol 424 (1-2) ◽  
pp. 157-163 ◽  
Author(s):  
Koji Urakami ◽  
Ken Hosoya ◽  
Anthony E. Beezer
Keyword(s):  
Liquid Chromatography ◽  
Stationary Phase ◽  
Mobile Phase ◽  
Enthalpies Of Transfer

Application of ionic liquids as mobile phase additives and surface-bonded stationary phase in liquid chromatography

2009 ◽  
Vol 26 (5) ◽  
pp. 1353-1358 ◽  
Author(s):  
Dandan Han ◽  
Minglei Tian ◽  
Dong Wha Park ◽  
Dae Ki Choi ◽  
Kyung Ho Row
Keyword(s):  
Ionic Liquids ◽  
Liquid Chromatography ◽  
Stationary Phase ◽  
Mobile Phase ◽  
Mobile Phase Additives

Preparative isolation of the eutomer of cyclothiazide by high-performance liquid chromatography on a cellulose-derived chiral stationary phase with toluene-acetone as the mobile phase

1996 ◽  
Vol 729 (1-2) ◽  
pp. 29-32 ◽  
Author(s):  
Laureano Oliveros ◽  
Cristina Minguillón ◽  
Bernard Serkiz ◽  
Florent Meunier ◽  
Jean-Pierre Volland ◽  
...  
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Stationary Phase ◽  
Mobile Phase ◽  
High Performance ◽  
Chiral Stationary Phase ◽  
Preparative Isolation

Sulfated Polyborate, a novel buffer for low pH mobile phase on a non-end capped stationary phase in reverse phase liquid chromatography

2021 ◽  
Vol 08 ◽  
Author(s):  
Purushottam Sutar ◽  
Pravin Khedkar ◽  
Ganesh Chaturbhuj
Keyword(s):  
Liquid Chromatography ◽  
Stationary Phase ◽  
Mobile Phase ◽  
Basic Drugs ◽  
Buffer Concentration ◽  
Organic Modifier ◽  
Reverse Phase ◽  
Sulfated Polyborate ◽  
Reverse Phase Liquid Chromatography ◽  
Phase Liquid

Background: Sulfated Polyborate, a novel inorganic material primarily designed as a catalyst, has shown properties such as high solubility in organic solvents, low U.V. cut-off, and pKa ≈2.0, which suggests its potential as a mobile phase buffer for reverse-phase liquid chromatography. Objective: This study aims to substantiate the role of Sulfated Polyborate as mobile phase buffer for reverse-phase liquid chromatographic analysis of basic drugs with high pKa values viz. Bisoprolol fumarate, Timolol maleate, Verapamil hydrochloride, and Carvedilol. Methods: Solubilities, U.V. cut-offs, and pKa of Sulfated Polyborate was first experimentally confirmed. The behaviour of Sulfated Polyborate as mobile phase buffer at pH 3.0 was ascertained by varying the buffer concentration, flow rates, and percent organic modifier for elution of the four basic drugs on a non-end capped octyl silyl (C8) column. Similarly, the study was performed with KH2PO4 as a reference buffer. The column performance and conductometric measurements ascertained the impact of Sulfated Polyborate on the stationary phase. Results: Sulfated Polyborate and KH2PO4 buffers showed correlation coefficients of 0.99 and 1.00 for analyte retention factors for variation of buffer concentration and organic modifier composition, respectively. Peak symmetries and the number of theoretical plates were improved from > 2.0 to < 2.0 and ≈1000 to ≈3000, respectively, for Variation in buffer concentrations. Similar Van Deemter plots indicated equivalency of Sulfated Polyborate and KH2PO4 buffers. The column performance and conductometric measurements depicted no adsorption on the stationary phase. Conclusion: The present study demonstrates Sulfated Polyborate as a novel buffer for analytes with higher pKa on reverse-phase liquid chromatography.

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