Weight titrimetry with equivalence point detection by differential electrolytic potentiometry (DEP)

1984 ◽  
Vol 56 (3) ◽  
pp. 589-591
Author(s):  
Pablo. Cofre ◽  
Georgina. Copia
1960 ◽  
Vol 32 (10) ◽  
pp. 1253-1257 ◽  
Author(s):  
D. L. Smith ◽  
D. R. Jamieson ◽  
P. J. Elving

1991 ◽  
Vol 7 (Supple) ◽  
pp. 753-754 ◽  
Author(s):  
EIKO NAKAMURA ◽  
YUKIO NAGAHARA ◽  
HIROSHI NAMIKI

1968 ◽  
Vol 20 (03/04) ◽  
pp. 548-554
Author(s):  
J Gajewski ◽  
G Markus

SummaryA method for the standardization of human plasminogen is proposed, based on the stoichiometric interaction between plasminogen and streptokinase, resulting in inhibition of proteolytic activity. Activation of a constant amount of plasminogen with increasing amounts of streptokinase yields linearly decreasing activities, as a function of streptokinase, with a sharp transition to a constant residual level. The point of transition corresponds to complete saturation of plasmin with streptokinase in a 1:1 molar ratio, and is therefore a measure of the amount of plasminogen present initially, in terms of streptokinase equivalents. The equivalence point is independent of the kind of protein substrate used, buffer, pH, length of digestion and, within limits, temperature. The method, therefore, is not subject to the variations commonly encountered in the usual determination based on specific activity measurements.


Sign in / Sign up

Export Citation Format

Share Document