Gas-Phase Separation of Drugs and Metabolites Using Modifier-Assisted Differential Ion Mobility Spectrometry Hyphenated to Liquid Extraction Surface Analysis and Mass Spectrometry

2013 ◽  
Vol 85 (24) ◽  
pp. 11771-11779 ◽  
Author(s):  
Tiffany Porta ◽  
Emmanuel Varesio ◽  
Gérard Hopfgartner
Keyword(s):  
Mass Spectrometry ◽  
Phase Separation ◽  
Gas Phase ◽  
Ion Mobility Spectrometry ◽  
Ion Mobility ◽  
Liquid Extraction ◽  
Differential Ion Mobility Spectrometry ◽  
Liquid Extraction Surface Analysis ◽  
Gas Phase Separation ◽  
Drugs And Metabolites

scholarly journals Liquid extraction surface analysis field asymmetric waveform ion mobility spectrometry mass spectrometry for the analysis of dried blood spots

The Analyst ◽  
2015 ◽  
Vol 140 (20) ◽  
pp. 6879-6885 ◽  
Author(s):  
Rian L. Griffiths ◽  
Alex Dexter ◽  
Andrew J. Creese ◽  
Helen J. Cooper
Keyword(s):  
Mass Spectrometry ◽  
Surface Analysis ◽  
Ion Mobility Spectrometry ◽  
Ion Mobility ◽  
High Field ◽  
Dried Blood Spots ◽  
Liquid Extraction ◽  
Asymmetric Waveform ◽  
Blood Spots ◽  
Liquid Extraction Surface Analysis

LESA mass spectrometry coupled with high field asymmetric waveform ion mobility spectrometry (FAIMS) for the analysis of dried blood spots.

scholarly journals Direct Measurement of Light and Heavy Antibody Chains Using Differential Ion Mobility Spectrometry and Middle-Down Mass Spectrometry

2019 ◽  
Author(s):  
Rafael D Melani ◽  
Kristina Srzentić ◽  
Vincent R Gerbasi ◽  
John P McGee ◽  
Romain Huguet ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Gas Phase ◽  
Ion Mobility Spectrometry ◽  
Ion Mobility ◽  
Asymmetric Waveform ◽  
Fast Fashion ◽  
Differential Ion Mobility Spectrometry ◽  
Ms Analysis ◽  
Polypeptide Sequence ◽  
Liquid Fractionation

AbstractThe analysis of monoclonal antibodies (mAbs) by a middle-down approach is a growing field that attracts the attention of many researchers and biopharma companies. Usually, liquid fractionation techniques are used to separate mAbs polypeptides chains before mass spectrometry (MS) analysis. Gas-phase fractionation techniques such as high-field asymmetric waveform ion mobility spectrometry (FAIMS) can replace liquid-based separations and reduce both analysis time and cost. Here, we present a rapid FAIMS tandem MS method capable of characterizing the polypeptide sequence of mAbs light (Lc) and heavy (Hc) chains in an unprecedented, easy, and fast fashion. This new method uses commercially available instruments and takes ∼ 24 minutes —40-60% faster than regular LC-MS/MS analysis — to acquire fragmentation data using different dissociation methods.

scholarly journals MALDI TIMS IMS of Disialoganglioside Isomers – GD1a and GD1b in Murine Brain Tissue

2021 ◽  
Author(s):  
Katerina Djambazova ◽  
Martin Dufresne ◽  
Lukasz Migas ◽  
Angela Kruse ◽  
Raf Van de Plas ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Phase Separation ◽  
Sialic Acid ◽  
Gas Phase ◽  
Sialic Acid Residue ◽  
Spinal Cord Tissue ◽  
Total Ganglioside ◽  
Tissue Sections ◽  
Maldi Ims ◽  
Gas Phase Separation

Gangliosides are classified as acidic glycosphingolipids, containing ceramide moieties and oligosaccharide chains with one or multiple sialic acid residue(s). The presence of multiple sialylation sites gives rise to highly diverse isomeric structures with distinct biological roles. Matrix-assisted laser desorption/ionization imaging mass spectrometry (MALDI IMS) enables the untargeted spatial analysis of gangliosides, among other biomolecules, directly from tissue sections. Integrating trapped ion mobility mass spectrometry (TIMS), a gas-phase separation technology, with MALDI IMS allows for the investi-gation of isomeric lipid structures in situ. Here we demonstrate the gas-phase separation of disialoganglioside isomers GD1a and GD1b that differ in the position of a sialic acid residue, in a standard mixture of both isomers, a total ganglioside extract, and directly from thin tissue sections. The unique spatial distributions of GD1a/b (d36:1) and GD1a/b (d38:1) were deter-mined from rat hippocampus, as well as in a spinal cord tissue section.

scholarly journals Gas-phase separation using a trapped ion mobility spectrometer

2011 ◽  
Vol 14 (2-3) ◽  
pp. 93-98 ◽  
Author(s):  
Francisco Fernandez-Lima ◽  
Desmond A. Kaplan ◽  
J. Suetering ◽  
Melvin A. Park
Keyword(s):  
Phase Separation ◽  
Gas Phase ◽  
Ion Mobility ◽  
Ion Mobility Spectrometer ◽  
Trapped Ion ◽  
Gas Phase Separation
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