Organic−Inorganic Hybrid Silica Monolith Based Immobilized Trypsin Reactor with High Enzymatic Activity

2008 ◽  
Vol 80 (8) ◽  
pp. 2949-2956 ◽  
Author(s):  
Junfeng Ma ◽  
Zhen Liang ◽  
Xiaoqiang Qiao ◽  
Qiliang Deng ◽  
Dingyin Tao ◽  
...  
Keyword(s):  
Enzymatic Activity ◽  
Inorganic Hybrid ◽  
Silica Monolith ◽  
Hybrid Silica ◽  
Immobilized Trypsin ◽  
High Enzymatic Activity

Facile synthesis of enzyme–inorganic hybrid nanoflowers and their application as an immobilized trypsin reactor for highly efficient protein digestion

RSC Advances ◽  
2014 ◽  
Vol 4 (27) ◽  
pp. 13888-13891 ◽  
Author(s):  
Zian Lin ◽  
Yun Xiao ◽  
Ling Wang ◽  
Yuqing Yin ◽  
Jiangnan Zheng ◽  
...  
Keyword(s):  
Enzymatic Activity ◽  
Immobilized Enzyme ◽  
Protein Digestion ◽  
Enzyme Reactor ◽  
Facile Synthesis ◽  
Inorganic Hybrid ◽  
Immobilized Enzyme Reactor ◽  
Highly Efficient ◽  
Novel Approach ◽  
Immobilized Trypsin

Hybrid nanoflowers were synthesized by a novel approach. The nanoflowers exhibited an enhanced enzymatic activity and can be used as an immobilized enzyme reactor (IMER) for highly efficient protein digestion.

Organic−Inorganic Hybrid Silica Monolith Based Immobilized Titanium Ion Affinity Chromatography Column for Analysis of Mitochondrial Phosphoproteome

2010 ◽  
Vol 9 (8) ◽  
pp. 4093-4101 ◽  
Author(s):  
Chunyan Hou ◽  
Junfeng Ma ◽  
Dingyin Tao ◽  
Yichu Shan ◽  
Zhen Liang ◽  
...  
Keyword(s):  
Affinity Chromatography ◽  
Inorganic Hybrid ◽  
Chromatography Column ◽  
Silica Monolith ◽  
Hybrid Silica

Efficient proteolysis using a regenerable metal-ion chelate immobilized enzyme reactor supported on organic-inorganic hybrid silica monolith

PROTEOMICS ◽  
2011 ◽  
Vol 11 (5) ◽  
pp. 991-995 ◽  
Author(s):  
Junfeng Ma ◽  
Chunyan Hou ◽  
Yu Liang ◽  
Tingting Wang ◽  
Zhen Liang ◽  
...  
Keyword(s):  
Metal Ion ◽  
Immobilized Enzyme ◽  
Enzyme Reactor ◽  
Inorganic Hybrid ◽  
Immobilized Enzyme Reactor ◽  
Silica Monolith ◽  
Hybrid Silica

Organic–inorganic hybrid silica materials containing imidazolium and dihydroimidazolium salts as recyclable organocatalysts for Knoevenagel condensations

2009 ◽  
Vol 11 (11) ◽  
pp. 1815 ◽  
Author(s):  
Montserrat Trilla ◽  
Roser Pleixats ◽  
Michel Wong Chi Man ◽  
Catherine Bied
Keyword(s):  
Inorganic Hybrid ◽  
Hybrid Silica ◽  
Silica Materials

Ultrafast fluorescence probe to H2O2 vapor based on organic-inorganic hybrid silica nanoparticles

Talanta ◽  
2021 ◽  
pp. 122914
Author(s):  
Hongbin Mu ◽  
Yimeng Zhang ◽  
Ping Zheng ◽  
Ming Zhang
Keyword(s):  
Silica Nanoparticles ◽  
Fluorescence Probe ◽  
Inorganic Hybrid ◽  
Hybrid Silica

scholarly journals Efficacy of Liquid Protein Hydrolysate from Chicken Feather by Pseudomonas sp. on the Growth of Cucumis sativus

2020 ◽  
Vol 2 (1) ◽  
pp. 16
Keyword(s):  
Escherichia Coli ◽  
Enzymatic Activity ◽  
Cucumis Sativus ◽  
Protein Hydrolysate ◽  
Antibacterial Properties ◽  
Full Potential ◽  
Pseudomonas Sp ◽  
Chicken Feather ◽  
Chicken Feathers ◽  
High Enzymatic Activity

Keratin, which made up the chicken feather, is difficult to be broken down by the proteolytic enzyme. Annually, millions of tons of chicken feathers are disposed of worldwide as waste without realizing the high protein content in the feather. Due to the presence of keratinase from keratinolytic bacteria, chicken feathers are disposed of together with poultry excreta. Therefore, this study is conducted to study the ability of liquid protein hydrolysate produced by bacteria in poultry excreta to utilize into biofertilizing and biocontrol. Keratinolytic bacteria are identified from poultry excreta by screening. The isolated enzyme was optimized in various conditions such as different pH, temperature, and feather concentration as well as nitrogen and carbon sources. Enzymatic activity increased gradually from 1% to 5% in carbon and nitrogen sources. Liquid protein hydrolysate was used to study the biofertilizing ability on the growth of Cucumis sativus and antibacterial effect on Escherichia coli. Pseudomonas sp. has the capability to degrade the feather on 10th day due to the high enzymatic activity. Pseudomonas sp. shows high enzymatic activity at 37⁰C, pH 8, and feather concentration at 0.5%. The chlorophyll estimation shows a p-value<0.05 after being treated with liquid protein hydrolysate. Liquid protein hydrolysate promoted the growth of Cucumis sativus as well as Pseudomonas sp. The antibacterial properties can also be seen against Escherichia coli. In a nutshell, chicken feather produces liquid protein hydrolysate, which has biofertilizing properties. The full potential of liquid hydrolysate can be understood with further analysis of peptide in protein hydrolysate.

A bifunctionalized organic–inorganic hybrid silica: synergistic effect enhances enantioselectivity

2012 ◽  
Vol 48 (97) ◽  
pp. 11898 ◽  
Author(s):  
Wei Xiao ◽  
Ronghua Jin ◽  
Tanyu Cheng ◽  
Daquan Xia ◽  
Hui Yao ◽  
...  
Keyword(s):  
Synergistic Effect ◽  
Inorganic Hybrid ◽  
Hybrid Silica
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