Efficient proteolysis using a regenerable metal-ion chelate immobilized enzyme reactor supported on organic-inorganic hybrid silica monolith

PROTEOMICS ◽  
2011 ◽  
Vol 11 (5) ◽  
pp. 991-995 ◽  
Author(s):  
Junfeng Ma ◽  
Chunyan Hou ◽  
Yu Liang ◽  
Tingting Wang ◽  
Zhen Liang ◽  
...  
Keyword(s):  
Metal Ion ◽  
Immobilized Enzyme ◽  
Enzyme Reactor ◽  
Inorganic Hybrid ◽  
Immobilized Enzyme Reactor ◽  
Silica Monolith ◽  
Hybrid Silica

Facile synthesis of enzyme–inorganic hybrid nanoflowers and their application as an immobilized trypsin reactor for highly efficient protein digestion

RSC Advances ◽  
2014 ◽  
Vol 4 (27) ◽  
pp. 13888-13891 ◽  
Author(s):  
Zian Lin ◽  
Yun Xiao ◽  
Ling Wang ◽  
Yuqing Yin ◽  
Jiangnan Zheng ◽  
...  
Keyword(s):  
Enzymatic Activity ◽  
Immobilized Enzyme ◽  
Protein Digestion ◽  
Enzyme Reactor ◽  
Facile Synthesis ◽  
Inorganic Hybrid ◽  
Immobilized Enzyme Reactor ◽  
Highly Efficient ◽  
Novel Approach ◽  
Immobilized Trypsin

Hybrid nanoflowers were synthesized by a novel approach. The nanoflowers exhibited an enhanced enzymatic activity and can be used as an immobilized enzyme reactor (IMER) for highly efficient protein digestion.

An enzyme–inorganic hybrid nanoflower based immobilized enzyme reactor with enhanced enzymatic activity

2015 ◽  
Vol 3 (11) ◽  
pp. 2295-2300 ◽  
Author(s):  
Yuqing Yin ◽  
Yun Xiao ◽  
Guo Lin ◽  
Qi Xiao ◽  
Zian Lin ◽  
...  
Keyword(s):  
Enzymatic Activity ◽  
Immobilized Enzyme ◽  
Protein Digestion ◽  
Enzyme Reactor ◽  
Inorganic Hybrid ◽  
Immobilized Enzyme Reactor ◽  
Highly Efficient

Ca3(PO4)2–ChT hybrid nanoflowers were synthesized by a facile approach. The nanoflowers exhibited an enhanced enzymatic activity and can be used as an immobilized enzyme reactor (IMER) for highly efficient protein digestion.

scholarly journals Study of the geometry of open channels in a layer-bed-type microfluidic immobilized enzyme reactor

2021 ◽  
Author(s):  
Cynthia Nagy ◽  
Robert Huszank ◽  
Attila Gaspar
Keyword(s):  
Immobilized Enzyme ◽  
High Surface ◽  
Volume Ratio ◽  
Volumetric Flow Rate ◽  
Enzyme Reactor ◽  
Channel Pattern ◽  
Immobilized Enzyme Reactor ◽  
Split Flow ◽  
Parallel Streams ◽  
Enzymatic Microreactors

AbstractThis paper aims at studying open channel geometries in a layer-bed-type immobilized enzyme reactor with computer-aided simulations. The main properties of these reactors are their simple channel pattern, simple immobilization procedure, regenerability, and disposability; all these features make these devices one of the simplest yet efficient enzymatic microreactors. The high surface-to-volume ratio of the reactor was achieved using narrow (25–75 μm wide) channels. The simulation demonstrated that curves support the mixing of solutions in the channel even in strong laminar flow conditions; thus, it is worth including several curves in the channel system. In the three different designs of microreactor proposed, the lengths of the channels were identical, but in two reactors, the liquid flow was split to 8 or 32 parallel streams at the inlet of the reactor. Despite their overall higher volumetric flow rate, the split-flow structures are advantageous due to the increased contact time. Saliva samples were used to test the efficiencies of the digestions in the microreactors. Graphical abstract

Organic−Inorganic Hybrid Silica Monolith Based Immobilized Trypsin Reactor with High Enzymatic Activity

2008 ◽  
Vol 80 (8) ◽  
pp. 2949-2956 ◽  
Author(s):  
Junfeng Ma ◽  
Zhen Liang ◽  
Xiaoqiang Qiao ◽  
Qiliang Deng ◽  
Dingyin Tao ◽  
...  
Keyword(s):  
Enzymatic Activity ◽  
Inorganic Hybrid ◽  
Silica Monolith ◽  
Hybrid Silica ◽  
Immobilized Trypsin ◽  
High Enzymatic Activity
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