Development of a Monoclonal Antibody against Ochratoxin A and Its Application in Enzyme-Linked Immunosorbent Assay and Gold Nanoparticle Immunochromatographic Strip

2008 ◽  
Vol 80 (18) ◽  
pp. 7029-7035 ◽  
Author(s):  
Biing-Hui Liu ◽  
Zih-Jay Tsao ◽  
Jing-Jhih Wang ◽  
Feng-Yih Yu
Keyword(s):  
Monoclonal Antibody ◽  
Gold Nanoparticle ◽  
Ochratoxin A ◽  
Immunosorbent Assay ◽  
Enzyme Linked Immunosorbent Assay ◽  
Immunochromatographic Strip

Identification of a high-affinity monoclonal antibody against ochratoxin A and its application in enzyme-linked immunosorbent assay

Toxicon ◽  
2015 ◽  
Vol 106 ◽  
pp. 89-96 ◽  
Author(s):  
Xian Zhang ◽  
Mengjiao Sun ◽  
Yue Kang ◽  
Hui Xie ◽  
Xin Wang ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Ochratoxin A ◽  
Immunosorbent Assay ◽  
Enzyme Linked Immunosorbent Assay ◽  
High Affinity

Production of a monoclonal antibody for the detection of vitamin B1 and its use in an indirect enzyme-linked immunosorbent assay and immunochromatographic strip

2020 ◽  
Vol 8 (9) ◽  
pp. 1935-1943 ◽  
Author(s):  
Lu Zeng ◽  
Xaioling Wu ◽  
Liqiang Liu ◽  
Liguang Xu ◽  
Hua Kuang ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Immunosorbent Assay ◽  
Colloidal Gold ◽  
Vitamin B1 ◽  
Enzyme Linked Immunosorbent Assay ◽  
Vitamin B ◽  
Immunochromatographic Test ◽  
Immunochromatographic Strip

A monoclonal antibody (mAb) against vitamin B1 was prepared and based on this, an indirect competitive enzyme-linked immunosorbent assay (ic-ELISA) and colloidal gold-based immunochromatographic test (ICT) strip were developed.

Rapid quantitative determination of fentanyl in human urine and serum using a gold-based immunochromatographic strip sensor

2020 ◽  
Vol 8 (37) ◽  
pp. 8573-8584
Author(s):  
Xianlu Lei ◽  
Xinxin Xu ◽  
Liqiang Liu ◽  
Hua Kuang ◽  
Liguang Xu ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Quantitative Determination ◽  
Immunosorbent Assay ◽  
Human Urine ◽  
Colloidal Gold ◽  
Enzyme Linked Immunosorbent Assay ◽  
Immunochromatographic Strip ◽  
Human Urine And Serum ◽  
Urine And Serum

In this study, an ultrasensitive monoclonal antibody (mAb) was prepared and used to develop an indirect competitive enzyme-linked immunosorbent assay (ic-ELISA) and a colloidal gold-based immunochromatographic strip (CG-ICS) for the analysis of fentanyl in urine and serum.

Ultrasensitive immunochromatographic strip assay for the detection of diminazene

The Analyst ◽  
2021 ◽  
Author(s):  
Mengjia chao ◽  
Xin-Xin Xu ◽  
Liqiang Liu ◽  
aihong wu ◽  
Shanshan Song ◽  
...  
Keyword(s):  
Monoclonal Antibodies ◽  
Gold Nanoparticle ◽  
Immunosorbent Assay ◽  
Lateral Flow ◽  
Enzyme Linked Immunosorbent Assay ◽  
Immunochromatographic Strip ◽  
Flow Test ◽  
Lateral Flow Test

We prepared monoclonal antibodies (mAbs) against diminazene and used them in the development of a gold nanoparticle-based lateral-flow test (GNT) strip and indirect competitive enzyme-linked immunosorbent assay for the detection...

scholarly journals Development of a Sensitive Enzyme-Linked Immunosorbent Assay and Rapid Gold Nanoparticle Immunochromatographic Strip for Detecting Citrinin in Monascus Fermented Food

Toxins ◽  
2018 ◽  
Vol 10 (9) ◽  
pp. 354 ◽  
Author(s):  
Shih-Wei Wu ◽  
Yao-An Yu ◽  
Biing-Hui Liu ◽  
Feng-Yih Yu
Keyword(s):  
Detection Limit ◽  
Gold Nanoparticle ◽  
Immunosorbent Assay ◽  
Keyhole Limpet Hemocyanin ◽  
Fermented Food ◽  
Enzyme Linked Immunosorbent Assay ◽  
Fermented Foods ◽  
Close Inspection ◽  
Immunochromatographic Strip ◽  
Rapid Antibody

Antibodies against citrinin (CTN) were generated from rabbits, which were injected with CTN-keyhole limpet hemocyanin (KLH). This work involved the development of a sensitive competitive direct enzyme-linked immunosorbent assay (cdELISA) and a rapid gold nanoparticle immunochromatographic strip (immunostrip) method for analyzing CTN in Monascus-fermented food. CTN at a concentration of 5.0 ng/mL caused 50% inhibition (IC50) of CTN-horseradish peroxidase (CTN-HRP) binding to the antibodies in the cdELISA. The capable on-site detection of CTN was accomplished by a rapid antibody-gold nanoparticle immunostrip with a detection limit of 20 ng/mL and that was completed within 15 min. A close inspection of 19 Monascus-fermented foods by cdELISA confirmed that 14 were contaminated with citrinin at levels from 28.6–9454 ng/g. Further analysis with the immunostrip is consistent with those results obtained using cdELISA. Both means are sensitive enough for the rapid examination of CTN in Monascus-fermented food products.

Determination of robenidine in shrimp and chicken samples using the indirect competitive enzyme-linked immunosorbent assay and immunochromatographic strip assay

The Analyst ◽  
2021 ◽  
Author(s):  
Lu Lin ◽  
Shanshan Song ◽  
Xiaoling Wu ◽  
Liqiang Liu ◽  
Hua Kuang ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Immunosorbent Assay ◽  
Rapid Screening ◽  
Enzyme Linked Immunosorbent Assay ◽  
Immunochromatographic Strip

In this study, the monoclonal antibody-based indirect competitive enzyme-linked immunosorbent assay (ic-ELISA) and an immunochromatographic strip assay were developed for the rapid screening of robenidine hydrochloride (ROBH) in samples.

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