Electrochemical sensors for β–adrenoceptor agonist isoprenaline analysis in human urine and serum samples using manganese cobalt oxide modified glassy carbon electrode

We demonstrated the simple and more accurate electrochemical sensor for isoprenaline (ISPT) analysis in pharmaceutical substances based on manganese cobalt oxide (MnCo2O4) modified with glassy carbon electrode (MCO/GCE). The nanomaterial...

Effects of Probiotics Administration on Human Metabolic Phenotype

The establishment of the beneficial interactions between the host and its microbiota is essential for the correct functioning of the organism, since microflora alterations can lead to many diseases. Probiotics improve balanced microbial communities, exerting substantial health-promoting effects. Here we monitored the molecular outcomes, obtained by gut microflora modulation through probiotic treatment, on human urine and serum metabolic profiles, with a metabolomic approach. Twenty-two subjects were enrolled in the study and administered with two different probiotic types, both singularly and in combination, for 8 weeks. Urine and serum samples were collected before and during the supplementation and were analyzed by nuclear magnetic resonance (NMR) spectroscopy and statistical analyses. After eight weeks of treatment, probiotics deeply influence the urinary metabolic profiles of the volunteers, without significantly altering their single phenotypes. Anyway, bacteria supplementation tends to reduce the differences in metabolic phenotypes among individuals. Overall, the effects are recipient-dependent, and in some individuals, robust effects are already well visible after four weeks. Modifications in metabolite levels, attributable to each type of probiotic administration, were also monitored. Metabolomic analysis of biofluids turns out to be a powerful technique to monitor the dynamic interactions between the microflora and the host, and the individual response to probiotic assumption.

Rapid quantitative determination of fentanyl in human urine and serum using a gold-based immunochromatographic strip sensor

In this study, an ultrasensitive monoclonal antibody (mAb) was prepared and used to develop an indirect competitive enzyme-linked immunosorbent assay (ic-ELISA) and a colloidal gold-based immunochromatographic strip (CG-ICS) for the analysis of fentanyl in urine and serum.

Visual detection of glucose based on quantum dots aerogel in microfluidic chips

In this study, we demonstrated a new optical glucose microfluidic sensor based on quantum dots (QDs) aerogels and glucose oxidase (GOx) for rapid, low-cost and quantitative detection of glucose in human urine and serum.