Implementation of a novel method for high-throughput screening of reactions in microdroplets. The reaction and analysis steps are performed simultaneously using desorption electrospray ionization mass spectrometry (DESI-MS) at a rate of up to 1 reaction mixture per second.
A reliable, label-free, ion suppression-free Sirtuin 1 assay has been developed. By interfacing multi-well plates to electrospray ionization mass spectrometry by oil-segmented droplets, the assay can be applied for high throughput Sirtuin 1 modulator screening.