Protein Crystallization in the Presence of a Metastable Liquid–Liquid Phase Separation

2020 ◽  
Vol 20 (12) ◽  
pp. 7951-7962
Author(s):  
Ralph Maier ◽  
Georg Zocher ◽  
Andrea Sauter ◽  
Stefano Da Vela ◽  
Olga Matsarskaia ◽  
...  
Keyword(s):  
Phase Separation ◽  
Liquid Phase ◽  
Protein Crystallization ◽  
Liquid Phase Separation ◽  
Metastable Liquid ◽  
Liquid Liquid Phase Separation

Reentrant condensation, liquid–liquid phase separation and crystallization in protein solutions induced by multivalent metal ions

2014 ◽  
Vol 86 (2) ◽  
pp. 191-202 ◽  
Author(s):  
Fajun Zhang ◽  
Felix Roosen-Runge ◽  
Andrea Sauter ◽  
Marcell Wolf ◽  
Robert M. J. Jacobs ◽  
...  
Keyword(s):  
Phase Separation ◽  
Liquid Phase ◽  
Metal Ions ◽  
Protein Interactions ◽  
Protein Crystallization ◽  
Liquid Phase Separation ◽  
Protein Solutions ◽  
Metastable Liquid ◽  
Multivalent Metal ◽  
Liquid Liquid Phase Separation

Abstract We briefly summarize the recent progress in tuning protein interactions as well as phase behavior in protein solutions using multivalent metal ions. We focus on the influence of control parameters and the mechanism of reentrant condensation, the metastable liquid–liquid phase separation and classical vs. non-classical pathways of protein crystallization.

The role of cluster formation and metastable liquid—liquid phase separation in protein crystallization

2012 ◽  
Vol 159 ◽  
pp. 313 ◽  
Author(s):  
Fajun Zhang ◽  
Felix Roosen-Runge ◽  
Andrea Sauter ◽  
Roland Roth ◽  
Maximilian W. A. Skoda ◽  
...  
Keyword(s):  
Phase Separation ◽  
Liquid Phase ◽  
Protein Crystallization ◽  
Cluster Formation ◽  
Liquid Phase Separation ◽  
Metastable Liquid ◽  
Liquid Liquid Phase Separation

Charge-controlled metastable liquid–liquid phase separation in protein solutions as a universal pathway towards crystallization

Soft Matter ◽  
2012 ◽  
Vol 8 (5) ◽  
pp. 1313-1316 ◽  
Author(s):  
Fajun Zhang ◽  
Roland Roth ◽  
Marcell Wolf ◽  
Felix Roosen-Runge ◽  
Maximilian W. A. Skoda ◽  
...  
Keyword(s):  
Phase Separation ◽  
Liquid Phase ◽  
Liquid Phase Separation ◽  
Protein Solutions ◽  
Metastable Liquid ◽  
Liquid Liquid Phase Separation

scholarly journals Interactions in protein solutions close to liquid-liquid phase separation: Ethanol reduces attractions via changes of the dielectric solution properties

2021 ◽  
Author(s):  
Jan Hansen ◽  
Rajeevann Uthayakumar ◽  
Jan Skov Pedersen ◽  
Stefan U. Egelhaaf ◽  
Florian Platten
Keyword(s):  
Phase Separation ◽  
Dielectric Properties ◽  
Liquid Phase ◽  
Protein Crystallization ◽  
Liquid Phase Separation ◽  
Protein Solutions ◽  
Solution Properties ◽  
Liquid Liquid Phase Separation

Ethanol is a common protein crystallization agent, precipitant, and denaturant, but also alters the dielectric properties of solutions. While ethanol-induced unfolding is largely ascribed to its hydrophobic parts, its effect...

Phase Transformations in ZrO2-SiO2 Gels

1990 ◽  
Vol 180 ◽  
Author(s):  
J. Campaniello ◽  
E. M. Rabinovich ◽  
P. Berthet ◽  
A. Revcolevschi ◽  
Nonna A. Kopylov
Keyword(s):  
Phase Separation ◽  
Liquid Phase ◽  
Phase Transformations ◽  
Tetraethyl Orthosilicate ◽  
Liquid Phase Separation ◽  
Metastable Liquid ◽  
Higher Temperature ◽  
Liquid Liquid Phase Separation

ABSTRACTTransparent sols and gels in the ZrO2-SiO2 system containing 25, 50 and 75 mole % ZrO2 were prepared using tetraethyl orthosilicate (TEOS) and Zr n-propoxide solution in the presence of acids. Heating the gels to 600°C yielded an amorphous product which at higher temperature crystallized with formation of tetragonal and monoclinic ZrO2 . Zircon crystallized slowly at 1600°C. The results are explained by metastable liquid-liquid phase separation in the studied system.

Ultrasonic Irradiation and Seeding To Prevent Metastable Liquid–Liquid Phase Separation and Intensify Crystallization

2018 ◽  
Vol 18 (4) ◽  
pp. 2628-2635 ◽  
Author(s):  
Zhenguo Gao ◽  
Fatima Altimimi ◽  
Junbo Gong ◽  
Ying Bao ◽  
Jingkang Wang ◽  
...  
Keyword(s):  
Phase Separation ◽  
Liquid Phase ◽  
Ultrasonic Irradiation ◽  
Liquid Phase Separation ◽  
Metastable Liquid ◽  
Liquid Liquid Phase Separation

scholarly journals Liquid-Liquid Phase Separation of Tau Driven by Hydrophobic Interaction Facilitates Fibrillization of Tau

2021 ◽  
Vol 433 (2) ◽  
pp. 166731
Author(s):  
Yanxian Lin ◽  
Yann Fichou ◽  
Andrew P. Longhini ◽  
Luana C. Llanes ◽  
Pengyi Yin ◽  
...  
Keyword(s):  
Phase Separation ◽  
Liquid Phase ◽  
Hydrophobic Interaction ◽  
Liquid Phase Separation ◽  
Liquid Liquid Phase Separation
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