Protein Crystallization As a Process Step in a Novel Meso Oscillatory Flow Reactor: Study of Lysozyme Phase Behavior

Abstract Oscillatory flow reactors (OFRs) superimpose an oscillatory flow to the net movement through a flow reactor. OFRs have been engineered to enable improved mixing, excellent heat- and mass transfer and good plug flow character under a broad range of operating conditions. Such features render these reactors appealing, since they are suitable for reactions that require long residence times, improved mass transfer (such as in biphasic liquid-liquid systems) or to homogeneously suspend solid particles. Various OFR configurations, offering specific features, have been developed over the past two decades, with significant progress still being made. This review outlines the principles and recent advances in OFR technology and overviews the synthetic applications of OFRs for liquid-liquid and solid-liquid biphasic systems.

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Towards Protein Crystallization as a Process Step in Downstream Processing of Therapeutic Antibodies: Screening and Optimization at Microbatch Scale

PLoS ONE ◽

10.1371/journal.pone.0025282 ◽

2011 ◽

Vol 6 (9) ◽

pp. e25282 ◽

Cited By ~ 35

Author(s):

Yuguo Zang ◽

Bernd Kammerer ◽

Maike Eisenkolb ◽

Katrin Lohr ◽

Hans Kiefer

Keyword(s):

Protein Crystallization ◽

Downstream Processing ◽

Therapeutic Antibodies ◽

Process Step

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Synthesis of palm based biolubricant in an oscillatory flow reactor (OFR)

Industrial Crops and Products ◽

10.1016/j.indcrop.2013.10.042 ◽

2014 ◽

Vol 52 ◽

pp. 567-574 ◽

Cited By ~ 25

Author(s):

May Ying Koh ◽

Tinia Idaty Mohd. Ghazi ◽

Azni Idris

Keyword(s):

Oscillatory Flow ◽

Flow Reactor

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Numerical Simulation Study on the Concentration Field in an Oscillatory Flow Reactor with Conic Ring Baffles

Applied Mechanics and Materials ◽

10.4028/www.scientific.net/amm.378.418 ◽

2013 ◽

Vol 378 ◽

pp. 418-423

Author(s):

Gang Liu ◽

Jia Wu ◽

Wei Li

Keyword(s):

Numerical Simulation ◽

Oscillatory Flow ◽

Flow Reactor ◽

Stokes Equations ◽

Three Dimensional ◽

Concentration Field ◽

Navier Stokes ◽

Navier Stokes Equations ◽

Turbulent Diffusion Coefficient ◽

Simulation Results

The three-dimensional construct of concentration field in an oscillatory flow reactor (OFR) containing periodically spaced conic ring baffles was investigated by numerical simulation employing Reynolds-averaged Navier-Stokes equations. The computation covered a range of Oscillatory Reynolds number (Reo) from 623.32 to 3116.58 at Strouhal number (St) 0.995 and 1.99. The contour of concentration field showed that the concentration in the most part of the channel is relative uniform and a small retention area is found below the conic ring baffles, which means a region of relative poor mixing. In addition, the turbulent diffusion coefficient calculated from the simulation results implied the greater oscillatory amplitude and oscillatory frequency superimposed to the fluid, the stronger is the turbulence intensity.

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