Immobilization-Stabilization of β-Glucosidase for Implementation of Intensified Hydrolysis of Cellobiose in Continuous Flow Reactors

Cellulose saccharification to glucose is an operation of paramount importance in the bioenergy sector and the chemical and food industries, while glucose is a critical platform chemical in the integrated biorefinery. Among the cellulose degrading enzymes, β-glucosidases are responsible for cellobiose hydrolysis, the final step in cellulose saccharification, which is usually the critical bottleneck for the whole cellulose saccharification process. The design of very active and stable β-glucosidase-based biocatalysts is a key strategy to implement an efficient saccharification process. Enzyme immobilization and reaction engineering are two fundamental tools for its understanding and implementation. Here, we have designed an immobilized-stabilized solid-supported β−glucosidase based on the glyoxyl immobilization chemistry applied in porous solid particles. The biocatalyst was stable at operational temperature and highly active, which allowed us to implement 25 °C as working temperature with a catalyst productivity of 109 mmol/min/gsupport. Cellobiose degradation was implemented in discontinuous stirred tank reactors, following which a simplified kinetic model was applied to assess the process limitations due to substrate and product inhibition. Finally, the reactive process was driven in a continuous flow fixed-bed reactor, achieving reaction intensification under mild operation conditions, reaching full cellobiose conversion of 34 g/L in a reaction time span of 20 min.

Short-Term Oxidation Behavior, Microstructure Evolution and Compression Behavior of Nickel-Based Superalloy GH4037 in Solid and Semi-Solid States

Semi-solid processing combines the advantages of traditional forging and casting methods, so it has received much attention recently. However, the research on semi-solid behaviors of Nickel-based superalloys has been rarely reported. In order to investigate the behaviors of Nickel-based superalloy at solid and semi-solid states, oxidation experiments, isothermal treatment experiments and deformation experiments of GH4037 alloy were studied. Short-term oxidation experiments of GH4037 alloy were carried out at a solid temperature (1200 °C) and a semi-solid temperature (1360 °C). The results indicated that the oxides formed at 1200 °C were mainly composed of TiO2, Cr2O3 and a small amount of spinels NiCr2O4, while the oxides formed at 1360 °C consisted of the spinels of NiCr2O4, NiWO4 and NiMoO4 besides TiO2 and Cr2O3. Microstructure evolution of GH4037 alloy after semi-solid isothermal treatment at 1370 °C and 1380 °C was studied. The results indicated that semi-solid microstructures consisted of equiaxed solid grains and liquid phases. The average grains size and shape factor of solid grains were affected by melting mechanism and grain growth mechanism. Compression behaviors of GH4037 alloy after compressed at 1200 °C and 1360 °C were investigated. The results indicated that the flow stress of 1360 °C decreased significantly compared to that of 1200 °C. The deformation zones in the specimens were divided into three parts: the difficult deformation zone, the large deformation zone, and the free deformation zone. At 1200 °C, the deformation mechanism was plastic deformation mechanism. At 1360 °C, sliding between solid particles (SS), liquid flow (LF), flow of liquid incorporating solid particles (FLS), plastic deformation of solid particles (PDS) coexisted in the compression specimen.

Phase Transformation of M2 High Speed Steel during Semi-Solid Cooling and Conventional Cooling

In this paper, the fundamental microstructure evolution of M2 high speed steel was investigated during semi-solid controlled cooling and conventional cooling, respectively. Semi-solid controlled cooling was conducted at 1260 °C with cooling rates from 0.1 to 10 °C/s, while conventional cooling was conducted at 1200 °C and 890 °C with different cooling rates. The continuous cooling transformation curves were plot according to the microstructure evolution. The results showed that microstructure transformation behavior of cooling structure in semi-solid temperature range was different from that of conventional process. For semi-solid specimen, the solid austenite dissolved more alloy elements, and the austenite stability was increased. The solid matrix was pearlite structure in the samples with cooling rate of 0.1 °C /s. When the cooling rate reached 1 °C/s, the granular pearlite disappeared and martensite lath was formed. The structure was relatively uniform, on which there were large carbide with regular shape. The solidified liquid phase showed a network shape surrounding the solid particles. The size of solid particles showed a decreasing trend with the increase of cooling rates. For conventional cooling process, the large eutectic M6C carbide and the small precipitated MC carbide could not be dissolved by austenitized at 890 °C. Increasing the austenitization temperature helped dissolving part of the carbides. The hardenability of M2 steel was high. The hardness has increased to a high level for both semi-solid and conventional specimens when cooling rate reached 1 °C/s. No obvious increase happened when cooling rate continued increasing.

Energy balance formation of sub-eutectoide fayalita at high pressures in particle separators

This article presents an approach to the problem of ceramic types adhesion, applying energy and matter balance to the established control volume (cyclone) with the use of mathematical formulas that are interrelated to develop mathematical calculations and establish a new mathematical model The first results are obtained by operating the energy balance considering the collision of particles, using the principle of conservation of energy, the first law of thermodynamics, in order to obtain information that allows describing the phenomena of thermoplasticity and creep, in the formation of adhesions, from a physicochemical and kinetic point of view, which will serve as the basis for understanding their effect. As a result, an energy value of 660 kJ / mol was obtained, sufficient energy to start the transformation of the solid particles to a state of thermo-flow that allows the adhesion phenomenon to be started. Keywords: Adhesion, energy balance, cyclones, elutriation, eutectoid, fayalite, thermoplasticity. References [1]O. Bustamante. “Dissipation of mechanical energy in the discharge of a hydrocyclone”. (Dyna, Ed.) The network of Scientific Journals of Latin America, the Caribbean, Spain, and Portugal, vol. 80 (181), Pages 136-143, 2013. [2]K.Petersen, P.Aldrich, and D.Van.,”Hydrocyclone underflow monitoring using image processing methods. Minerals Engineering”, pp. 301-315,1996. [3]M. Farghaly,” Controlled Wash Water Injection to the hydrocyclone underflow” [Ph.D. Thesis]. Erlangen, FAU, 2009. [4]M, Schneider, and T. Neesse. “Overflow-control system for a hydrocyclone battery. Int. J. Miner. Process". 74, pp. 339 – 343, 2004. [5]J.Bergström., “Flow field and fiber fractionation studies in hydro cyclones” [Ph.D. Thesis] Stockholm, Sweden, Royal Institute of Technology, 2006. [6]C, Liu, L. Wang, and Q. Lui., “Investigation of energy loss mechanisms in cyclone separators”. Chemical Engineering Technology 28, pp. 1182-1190, 2005. [7]O.Dam. & E.Jeffes.,.”Model for detailed assessment of chemical composition of reduced iron ores from single measurement”. Ironmaking and Steelmaking, 1987. [8]E. Ringdalen., “Softening and melting of SiO2 an important parameter for reactions with quartz in Si production” pp 43-44, 2016.

A 3D DEM Model for Air Sparging Technology in the Saturated Granular Soils

This work provides a three-dimensional discrete element simulation (DEM) model to study the air sparging technology. The simulations have taken into account the multi-phases of bubble (gas) - fluid (water) - soil (solid) particles. Bubbles are treated as discrete individual particles, with buoyancy and drag forces applied to bubbles and soil particles. The trajectory of each discrete bubble particle can be tracked using the discrete element model. It is found that the diffusion of the whole bubble is inverted conical though the motion behavior of a single bubble particle is random. Furthermore, the distribution of the radius of influence (ROI) is not uniform. The bubbles become more concentrated as in the center of the inverted cone. The number of bubbles dissipated from the water surface is normally distributed. The DEM simulation is a novel approach to studying air sparging technology that can provide us a deeper insight into bubble migration at the microscopic level.

Impact of Molecular Chain Structure of Suspension Phase on Giant Electrorheological Performance

We demonstrate the impact of diester structure, in particular the alkyl chain length and branching structure, on the giant electrorheological (GER) effect and suspension stability. The existence of oil-particles interaction is of critical importance to induce the GER effect. To quantify GER performance and colloidal stability, we examine the yield stress, current density, field-off viscosity and sedimentation ratio with respect to the variation of chain length and branching structure. The oil-particles interaction is quantitatively analyzed by investigating the cluster size of particles in different diesters by a multiple light scattering analyzer, along with the wettability of different chain lengths of diesters and solid particles by the Washburn method. Our results indicate that long chain lengths favor the formation of particle agglomerates, thereby enhancing the GER effect (such as high yield stress). The attachment of branches on diester causes the formation of electronic correlation between branches and main chain, depending on the position of branches located, and hence results in superior GER performance and favorable suspension stability. An optimal GER fluid constituted by bis(2-ethylhexyl) sebacate is acquired with the achieved yield stress of 113 kPa at electric field strength of 4 kV/ mm and the prominent integrated GER properties.

Assessment of Peak Expiratory Flow Rate in Spice Mill Workers - A Cross-Sectional Study

Background: Numerous industries have researched the effects of occupational dust and particles on respiratory function. The continuous exposure to dispersed particles causes respiratory ailments in spice mill workers. Spice dust exposure is linked to a systemic inflammatory response, including respiratory irritation. Spice dust is finely divided solid particles and a form of respirable dust this is the leading cause of occupational disease. Allergies and asthma have been linked to spice mill workers. Objective: To study the effect of spice dust exposure on expiratory function and to compare the peak expiratory flow rate of spice mill workers and normal adults across age groups and gender Method: 186 subjects in Mumbai region, 93 spice mill workers and 93 normal adults were selected as per inclusion and exclusion criteria. PEFR was measured in all the participants using a mini Wright peak flow meter. Result: The statistical analysis showed that there is a significant difference in the peak expiratory flow rates of spice mill workers. However, intergroup analysis between age groups showed that there was not much difference in the PEFR values for the age group 40-50 when compared with normal. The reason could be less number of participants available for the study. Also, the gender-wise comparison showed statistically significant difference in male and female PEFR values. Conclusion: The present study concluded that peak expiratory flow rate was significantly reduced in spice mill workers when compared to normal adults of same age. Key words: PEFR, spice mill workers, spice dust, allergies.

Effect of Nucleant Particle Agglomeration on Grain Size

Solute accumulation/depletion in the liquid around a growing solid particle during the solidification of metallic melts creates a constitutionally supercooled (CS) zone that has a significant effect on the final solidified grain structure. In this paper, we introduce two mechanisms related to the CS zone that affect grain size: one is the grain initiation free zone (GIFZ) that describes the inability of nucleant particles located in the CS zone for grain initiation and the other is re-melting (RM) of solid particles due to overlap of CS zones. Based on these two mechanisms, we have systematically analysed the effect of nucleant particle agglomeration on grain size. We found that nucleant particle agglomeration has a significant effect on grain size and is responsible for the discrepancy between theoretically predicted grain size and the experimental data. In addition, our numerical analysis suggests that under normal solidification conditions relevant to industrial practice solid particle re-melting has little effect on grain size and thus may be ignored during theoretical analysis. A practical implication from this work is that significant grain refinement can be achieved by dispersing the nucleant particles in the melt prior to solidification.