Temperature-Tolerated Mainstream Nitrogen Removal by Anammox and Nitrite/Nitrate-Dependent Anaerobic Methane Oxidation in a Membrane Biofilm Reactor

Methane would potentially be an inexpensive, widely available electron donor for denitrification of wastewaters poor in organics. Currently, no methanotrophic microbe is known to denitrify. However, aerobic methane oxidation coupled to denitrification (AME-D) has been observed in several laboratory studies. In the AME-D process, aerobic methanotrophs oxidise methane and release organic metabolites and lysis products, which are used by coexisting denitrifiers as electron donors for denitrification. Due to the presence of oxygen, the denitrification efficiency in terms of methane-to-nitrate consumption is usually low. To improve this efficiency the use of a membrane biofilm reactor was investigated. The denitrification efficiency of an AME-D culture in (1) a suspended growth reactor, and (2) a membrane biofilm reactor was studied. The methane-to-nitrate consumption ratio for the suspended culture was 8.7. For the membrane-attached culture the ratio was 2.2. The results clearly indicated that the membrane-attached biofilm was superior to the suspended culture in terms of denitrification efficiency. This study showed that for practical application of the AME-D process, focus should be placed on development of a biofilm reactor.

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Aerobic methane oxidation coupled to denitrification in a membrane biofilm reactor: Treatment performance and the effect of oxygen ventilation

Bioresource Technology ◽

10.1016/j.biortech.2013.03.115 ◽

2013 ◽

Vol 145 ◽

pp. 2-9 ◽

Cited By ~ 28

Author(s):

Fei-yun Sun ◽

Wen-yi Dong ◽

Ming-fei Shao ◽

Xiao-mei Lv ◽

Ji Li ◽

...

Keyword(s):

Methane Oxidation ◽

Biofilm Reactor ◽

Treatment Performance ◽

Membrane Biofilm Reactor ◽

Effect Of Oxygen

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Nitrate/nitrite dependent anaerobic methane oxidation coupling with anammox in membrane biotrickling filter for nitrogen removal

Environmental Research ◽

10.1016/j.envres.2020.110533 ◽

2021 ◽

Vol 193 ◽

pp. 110533

Author(s):

Sheng-Qiang Fan ◽

Guo-Jun Xie ◽

Yang Lu ◽

Bing-Feng Liu ◽

De-Feng Xing ◽

...

Keyword(s):

Methane Oxidation ◽

Nitrogen Removal ◽

Anaerobic Methane Oxidation ◽

Biotrickling Filter ◽

Oxidation Coupling

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Achieving high nitrogen removal efficiency by optimizing nitrite-dependent anaerobic methane oxidation process with growth factors

Water Research ◽

10.1016/j.watres.2019.05.101 ◽

2019 ◽

Vol 161 ◽

pp. 35-42 ◽

Cited By ~ 8

Author(s):

Jiaqi Wang ◽

Miaolian Hua ◽

Yufen Li ◽

Fang Ma ◽

Ping Zheng ◽

...

Keyword(s):

Growth Factors ◽

Methane Oxidation ◽

Removal Efficiency ◽

Nitrogen Removal ◽

Oxidation Process ◽

Anaerobic Methane Oxidation ◽

High Nitrogen

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Response mechanism of denitrifying anaerobic methane oxidation microorganisms to ammonia

Environmental Chemistry ◽

10.1071/en19127 ◽

2020 ◽

Vol 17 (1) ◽

pp. 17 ◽

Cited By ~ 1

Author(s):

Juqing Lou ◽

Jiaping Li ◽

Xilei Wang

Keyword(s):

Methane Oxidation ◽

Nitrogen Removal ◽

Experimental Studies ◽

Ammonia Concentration ◽

Anaerobic Methane Oxidation ◽

Limiting Factor ◽

Response Mechanism ◽

Short Term ◽

Damo Bacteria

Environmental contextDenitrifying anaerobic methane oxidation (DAMO) is a new process in wastewater treatment with the potential to provide cheap and sustainable development. To better apply this technology to the large scale, we studied the response mechanism of DAMO microorganisms to ammonia, the main form of nitrogen in the nitrogenous wastewater. The results can provide a theoretical basis for the stable and efficient operation of DAMO processes. AbstractThe dominant microorganisms in the denitrifying anaerobic methane oxidation (DAMO) process are primarily DAMO bacteria and DAMO archaea, which can simultaneously realise methane oxidation and denitrification. Ammonia is the primary form of nitrogen found in wastewater. This study focuses on a coexistence system that contains both DAMO bacteria and DAMO archaea (DAMO co-system). The short- and long-term effects of NH4+-N on the DAMO co-system were investigated at both the macro level (such as denitrification performance) and the micro level (such as microbial structure and community). Short-term experimental studies demonstrated that the safe concentration of ammonia for this system was 250mgNL−1. When the ammonia concentration was 500mgNL−1, the nitrogen removal efficiency was significantly inhibited. With an increase in concentration and an extension of time, the inhibitory effect of ammonia was enhanced. Long-term experimental studies showed that the nitrogen removal performance of DAMO was completely inhibited when the ammonia concentration reached 1000mgNL−1 and that ammonia had a toxic accumulation effect on the DAMO co-system. The results of the pH experimental study demonstrated that free ammonia (FA) was the limiting factor in the alkaline condition, while ionised NH4+ was the limiting factor in neutral and acidic conditions. Scanning electron microscopy (SEM) demonstrated that the microbes in the DAMO co-system shrank after short-term exposure and that the microorganisms shrank in the shape of polygons. High-throughput sequencing analysis demonstrated that the community structure of the DAMO co-system changed substantially, and the species diversity and abundance decreased distinctly after long-term inhibition. A genus analysis indicated that the reduction in Nitrospirae may be an internal reason for the decrease in the denitrification performance of the DAMO co-system.

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