Computational and Experimental Approaches to Decipher the Binding Mechanism of General Odorant-Binding Protein 2 from Athetis lepigone to Chlorpyrifos and Phoxim

2020 ◽  
Author(s):  
Ya-Nan Zhang ◽  
Xiao-Chun Zhang ◽  
Rui Zhu ◽  
Wei-Chen Yao ◽  
Ji-Wei Xu ◽  
...  
Keyword(s):  
Binding Protein ◽  
Odorant Binding Protein ◽  
Binding Mechanism ◽  
Experimental Approaches ◽  
Odorant Binding

scholarly journals Odorant-binding protein. Characterization of ligand binding.

1990 ◽  
Vol 265 (11) ◽  
pp. 6118-6125
Author(s):  
J Pevsner ◽  
V Hou ◽  
A M Snowman ◽  
S H Snyder
Keyword(s):  
Ligand Binding ◽  
Binding Protein ◽  
Protein Characterization ◽  
Odorant Binding Protein ◽  
Odorant Binding

Molecular and Functional Characterization of One Odorant-Binding Protein Gene OBP3 in Bemisia tabaci (Hemiptera: Aleyrodidae)

2019 ◽  
Author(s):  
Ran Wang ◽  
Yuan Hu ◽  
Peiling Wei ◽  
Cheng Qu ◽  
Chen Luo
Keyword(s):  
Host Plant ◽  
Bemisia Tabaci ◽  
Binding Protein ◽  
Insect Pest ◽  
Critical Role ◽  
Functional Characterization ◽  
Odorant Binding Protein ◽  
Binding Characteristics ◽  
And Function ◽  
Odorant Binding

Abstract Odorant binding proteins (OBPs) of insects play a critical role in chemical perceptions and choice of insect host plant. Bemisia tabaci is a notorious insect pest which can damage more than 600 plant species. In order to explore functions of OBPs in B. tabaci, here we investigated binding characteristics and function of odorant-binding protein 3 in B. tabaci (BtabOBP3). The results indicated that BtabOBP3 shows highly similar sequence with OBPs of other insects, including the typical signature motif of six cysteines. The recombinant BtabOBP3 protein was obtained, and the evaluation of binding affinities to tested volatiles of host plant was conducted, then the results indicated that β-ionone had significantly higher binding to BtabOBP3 among other tested plant volatiles. Furthermore, silencing of BtabOBP3 significantly altered choice behavior of B. tabaci to β-ionone. In conclusion, it has been demonstrated that BtabOBP3 exerts function as one carrier of β-ionone and the results could be contributed to reveal the mechanisms of choosing host plant in B. tabaci.

scholarly journals Molecular and Functional Characterization of Three Odorant-Binding Protein from Periplaneta americana

PLoS ONE ◽  
2017 ◽  
Vol 12 (1) ◽  
pp. e0170072 ◽  
Author(s):  
Zhao-Qun Li ◽  
Peng He ◽  
Ya-Nan Zhang ◽  
Shuang-Lin Dong
Keyword(s):  
Periplaneta Americana ◽  
Binding Protein ◽  
Functional Characterization ◽  
Odorant Binding Protein ◽  
Odorant Binding

Identification and expression profile analysis of odorant-binding protein genes in Apolygus lucorum (Hemiptera: Miridae)

2013 ◽  
Vol 48 (3) ◽  
pp. 301-311 ◽  
Author(s):  
Ping Ji ◽  
Shao-Hua Gu ◽  
Jing-Tao Liu ◽  
Xiao-Qiang Zhu ◽  
Yu-Yuan Guo ◽  
...  
Keyword(s):  
Expression Profile ◽  
Profile Analysis ◽  
Binding Protein ◽  
Odorant Binding Protein ◽  
Apolygus Lucorum ◽  
Expression Profile Analysis ◽  
Odorant Binding
Sign in / Sign up

Export Citation Format

Share Document