Rates and Routes of Electron Transfer of [NiFe]-Hydrogenase in an Enzymatic Fuel Cell

Direct electron transfer (DET), which requires no mediator to shuttle electrons from enzyme active site to the electrode surface, minimizes complexity caused by the mediator and can further enable miniaturization for biocompatible and implantable devices. However, because the redox cofactors are typically deeply embedded in the protein matrix of the enzymes, electrons generated from oxidation reaction cannot easily transfer to the electrode surface. In this review, methods to improve the DET rate for enhancement of enzymatic fuel cell performances are summarized, with a focus on the more recent works (past 10 years). Finally, progress on the application of DET-enabled EFC to some biomedical and implantable devices are reported.

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Miniature Direct Electron Transfer Based Enzymatic Fuel Cell Operating in Human Sweat and Saliva

Fuel Cells ◽

10.1002/fuce.201400037 ◽

2014 ◽

Vol 14 (6) ◽

pp. 1050-1056 ◽

Cited By ~ 28

Author(s):

M. Falk ◽

D. Pankratov ◽

L. Lindh ◽

T. Arnebrant ◽

S. Shleev

Keyword(s):

Electron Transfer ◽

Fuel Cell ◽

Direct Electron Transfer ◽

Human Sweat ◽

Enzymatic Fuel Cell

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Enzyme orientation for direct electron transfer in an enzymatic fuel cell with alcohol oxidase and laccase electrodes

Biosensors and Bioelectronics ◽

10.1016/j.bios.2014.06.009 ◽

2014 ◽

Vol 61 ◽

pp. 569-574 ◽

Cited By ~ 24

Author(s):

Andrés A. Arrocha ◽

Ulises Cano-Castillo ◽

Sergio A. Aguila ◽

Rafael Vazquez-Duhalt

Keyword(s):

Electron Transfer ◽

Fuel Cell ◽

Direct Electron Transfer ◽

Alcohol Oxidase ◽

Enzymatic Fuel Cell

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Novel Graphene-Modified Poly(styrene-b-isoprene-b-styrene) Enzymatic Fuel Cell with Operation in Plant Leaves

Analytical Letters ◽

10.1080/00032719.2016.1143478 ◽

2016 ◽

Vol 49 (14) ◽

pp. 2322-2336 ◽

Cited By ~ 8

Author(s):

Seyda Korkut ◽

Muhammet Samet Kilic ◽

Sinan Uzuncar ◽

Baki Hazer

Keyword(s):

Fuel Cell ◽

Plant Leaves ◽

Enzymatic Fuel Cell

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Lack of Electricity Production by Pelobacter carbinolicus Indicates that the Capacity for Fe(III) Oxide Reduction Does Not Necessarily Confer Electron Transfer Ability to Fuel Cell Anodes

Applied and Environmental Microbiology ◽

10.1128/aem.00804-07 ◽

2007 ◽

Vol 73 (16) ◽

pp. 5347-5353 ◽

Cited By ~ 93

Author(s):

Hanno Richter ◽

Martin Lanthier ◽

Kelly P. Nevin ◽

Derek R. Lovley

Keyword(s):

Electron Transfer ◽

Fuel Cells ◽

Fuel Cell ◽

Microbial Fuel Cell ◽

Microbial Fuel Cells ◽

Electron Donors ◽

Rrna Genes ◽

Anode Surface ◽

Oxide Reduction ◽

Fuel Cell Anodes

ABSTRACT The ability of Pelobacter carbinolicus to oxidize electron donors with electron transfer to the anodes of microbial fuel cells was evaluated because microorganisms closely related to Pelobacter species are generally abundant on the anodes of microbial fuel cells harvesting electricity from aquatic sediments. P. carbinolicus could not produce current in a microbial fuel cell with electron donors which support Fe(III) oxide reduction by this organism. Current was produced using a coculture of P. carbinolicus and Geobacter sulfurreducens with ethanol as the fuel. Ethanol consumption was associated with the transitory accumulation of acetate and hydrogen. G. sulfurreducens alone could not metabolize ethanol, suggesting that P. carbinolicus grew in the fuel cell by converting ethanol to hydrogen and acetate, which G. sulfurreducens oxidized with electron transfer to the anode. Up to 83% of the electrons available in ethanol were recovered as electricity and in the metabolic intermediate acetate. Hydrogen consumption by G. sulfurreducens was important for ethanol metabolism by P. carbinolicus. Confocal microscopy and analysis of 16S rRNA genes revealed that half of the cells growing on the anode surface were P. carbinolicus, but there was a nearly equal number of planktonic cells of P. carbinolicus. In contrast, G. sulfurreducens was primarily attached to the anode. P. carbinolicus represents the first Fe(III) oxide-reducing microorganism found to be unable to produce current in a microbial fuel cell, providing the first suggestion that the mechanisms for extracellular electron transfer to Fe(III) oxides and fuel cell anodes may be different.

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