Applications of Protein Fragment Complementation Assays for Analyzing Biomolecular Interactions and Biochemical Networks in Living Cells

Real-Time Protein-Fragment Complementation Assays for Studying Temporal, Spatial, and Spatiotemporal Dynamics of Protein–Protein Interactions in Living Cells

Cold Spring Harbor Protocols ◽

10.1101/pdb.prot090068 ◽

2016 ◽

Vol 2016 (11) ◽

pp. pdb.prot090068 ◽

Cited By ~ 2

Author(s):

Mohan Malleshaiah ◽

Emmanuelle Tchekanda ◽

Stephen W. Michnick

Keyword(s):

Real Time ◽

Protein Interactions ◽

Living Cells ◽

Spatiotemporal Dynamics ◽

Protein Fragment ◽

Extended Linkers Improve the Detection of Protein-protein Interactions (PPIs) by Dihydrofolate Reductase Protein-fragment Complementation Assay (DHFR PCA) in Living Cells

Molecular & Cellular Proteomics ◽

10.1074/mcp.tir117.000385 ◽

2017 ◽

Vol 17 (2) ◽

pp. 373-383 ◽

Cited By ~ 5

Author(s):

Andrée-Ève Chrétien ◽

Isabelle Gagnon-Arsenault ◽

Alexandre K. Dubé ◽

Xavier Barbeau ◽

Philippe C. Després ◽

...

Keyword(s):

Dihydrofolate Reductase ◽

Protein Interactions ◽

Living Cells ◽

Protein Fragment ◽

Complementation Assay ◽

Corrigendum: Real-Time Protein-Fragment Complementation Assays for Studying Temporal, Spatial, and Spatiotemporal Dynamics of Protein–Protein Interactions in Living Cells

Cold Spring Harbor Protocols ◽

10.1101/pdb.corr107815 ◽

2022 ◽

Vol 2022 (1) ◽

pp. pdb.corr107815

Author(s):

Mohan Malleshaiah ◽

Emmanuelle Tchekanda ◽

Stephen W. Michnick

Keyword(s):

Real Time ◽

Protein Interactions ◽

Living Cells ◽

Spatiotemporal Dynamics ◽

Protein Fragment ◽

Mapping Biochemical Networks With Protein-Fragment Complementation Assays

Protein-Protein Interactions ◽

10.1385/1-59259-762-9:411 ◽

2004 ◽

pp. 411-426 ◽

Cited By ~ 6

Author(s):

Ingrid Remy ◽

Stephen W. Michnick

Keyword(s):

Biochemical Networks ◽

Protein Fragment ◽

Extended linkers improve the detection of protein-protein interactions (PPIs) by dihydrofolate reductase protein-fragment complementation assay (DHFR PCA) in living cells

Molecular & Cellular Proteomics ◽

10.1074/mcp.a117.000385 ◽

2018 ◽

Vol 17 (3) ◽

pp. 549-549 ◽

Cited By ~ 5

Author(s):

Andrée-Ève Chrétien ◽

Isabelle Gagnon-Arsenault ◽

Alexandre K. Dubé ◽

Xavier Barbeau ◽

Philippe C. Després ◽

...

Keyword(s):

Dihydrofolate Reductase ◽

Protein Interactions ◽

Living Cells ◽

Protein Fragment ◽

Complementation Assay ◽

Protein-Fragment Complementation Assays for Large-Scale Analysis, Functional Dissection and Dynamic Studies of Protein–Protein Interactions in Living Cells

Methods in Molecular Biology - Signal Transduction Protocols ◽

10.1007/978-1-61779-160-4_25 ◽

2011 ◽

pp. 395-425 ◽

Cited By ~ 20

Author(s):

Stephen W. Michnick ◽

Po Hien Ear ◽

Christian Landry ◽

Mohan K. Malleshaiah ◽

Vincent Messier

Keyword(s):

Protein Interactions ◽

Large Scale ◽

Living Cells ◽

Scale Analysis ◽

Protein Fragment ◽

Dynamic Studies ◽

Large Scale Analysis ◽

Genome-wide Protein-protein Interaction Screening by Protein-fragment Complementation Assay (PCA) in Living Cells

Journal of Visualized Experiments ◽

10.3791/52255 ◽

2015 ◽

Cited By ~ 9

Author(s):

Samuel Rochette ◽

Guillaume Diss ◽

Marie Filteau ◽

Jean-Baptiste Leducq ◽

Alexandre K. Dubé ◽

...

Keyword(s):

Protein Interaction ◽

Living Cells ◽

Protein Fragment ◽

Complementation Assay ◽

Protein Protein Interaction ◽

Genome Wide ◽

Interaction Screening ◽

Methods in Enzymology - Guide to Yeast Genetics: Functional Genomics, Proteomics, and Other Systems Analysis ◽

A Toolkit of Protein-Fragment Complementation Assays for Studying and Dissecting Large-Scale and Dynamic Protein–Protein Interactions in Living Cells

10.1016/s0076-6879(10)70014-8 ◽

2010 ◽

pp. 335-368 ◽

Cited By ~ 28

Author(s):

Stephen W. Michnick ◽

Po Hien Ear ◽

Christian Landry ◽

Mohan K. Malleshaiah ◽

Vincent Messier

Keyword(s):

Protein Interactions ◽

Large Scale ◽

Living Cells ◽

Protein Fragment ◽

Extended Linkers Improve the Detection of Protein-protein Interactions (PPIs) by Dihydrofolate Reductase Protein-fragment Complementation Assay (DHFR PCA) in Living Cells

Molecular & Cellular Proteomics ◽

10.1074/mcp.ra117.000385 ◽

2018 ◽

Vol 17 (2) ◽

pp. 373-383

Author(s):

Andrée-Ève Chrétien ◽

Isabelle Gagnon-Arsenault ◽

Alexandre K. Dubé ◽

Xavier Barbeau ◽

Philippe C. Després ◽

...

Keyword(s):

Dihydrofolate Reductase ◽

Protein Interactions ◽

Living Cells ◽

Protein Fragment ◽

Complementation Assay ◽

Mapping Biochemical Networks with Protein Fragment Complementation Assays

Methods in Molecular Biology - Protein-Protein Interactions ◽

10.1007/978-1-4939-2425-7_31 ◽

2015 ◽

pp. 467-481 ◽

Cited By ~ 11

Author(s):

Ingrid Remy ◽

Stephen W. Michnick

Keyword(s):

Biochemical Networks ◽

Protein Fragment ◽