Single-Base Resolution Mapping Reveals Distinct 5-Formylcytidine in Saccharomyces cerevisiae mRNAs

2021 ◽  
Author(s):  
Yafen Wang ◽  
Zonggui Chen ◽  
Xiong Zhang ◽  
Xiaocheng Weng ◽  
Jikai Deng ◽  
...  
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Single Base ◽  
Resolution Mapping ◽  
Single Base Resolution

scholarly journals Single-Base Resolution Mapping of 5-Hydroxymethylcytosine Modifications in Hippocampus of Alzheimer’s Disease Subjects

2017 ◽  
Vol 63 (2) ◽  
pp. 185-197 ◽  
Author(s):  
Elizabeth M. Ellison ◽  
Melissa A. Bradley-Whitman ◽  
Mark A. Lovell
Keyword(s):  
Alzheimer’S Disease ◽  
Alzheimer's Disease ◽  
Single Base ◽  
Resolution Mapping ◽  
Single Base Resolution

scholarly journals High-throughput single-base resolution mapping of RNA 2΄-O-methylated residues

2016 ◽  
Vol 45 (3) ◽  
pp. 1433-1441 ◽  
Author(s):  
Danny Incarnato ◽  
Francesca Anselmi ◽  
Edoardo Morandi ◽  
Francesco Neri ◽  
Mara Maldotti ◽  
...  
Keyword(s):  
High Throughput ◽  
Single Base ◽  
Resolution Mapping ◽  
Single Base Resolution

scholarly journals Single-base resolution mapping of H1-nucleosome interactions and 3D organization of the nucleosome

2010 ◽  
Vol 107 (21) ◽  
pp. 9620-9625 ◽  
Author(s):  
S. H. Syed ◽  
D. Goutte-Gattat ◽  
N. Becker ◽  
S. Meyer ◽  
M. S. Shukla ◽  
...  
Keyword(s):  
Single Base ◽  
Resolution Mapping ◽  
Single Base Resolution

scholarly journals Single base resolution mapping of 2′-O-methylation sites in human mRNA and in 3′ terminal ends of small RNAs

Methods ◽  
2019 ◽  
Vol 156 ◽  
pp. 85-90 ◽  
Author(s):  
Phillip J. Hsu ◽  
Qili Fei ◽  
Qing Dai ◽  
Hailing Shi ◽  
Dan Dominissini ◽  
...  
Keyword(s):  
Small Rnas ◽  
Single Base ◽  
Resolution Mapping ◽  
Single Base Resolution

scholarly journals Single-Base Resolution: Increasing the Specificity of the CRISPR-Cas System in Gene Editing

2020 ◽  
Author(s):  
Roy Rabinowitz ◽  
Daniel Offen
Keyword(s):  
Gene Editing ◽  
Single Base ◽  
Single Base Resolution

scholarly journals Noncanonical crRNAs derived from host transcripts enable multiplexable RNA detection by Cas9

Science ◽  
2021 ◽  
pp. eabe7106
Author(s):  
Chunlei Jiao ◽  
Sahil Sharma ◽  
Gaurav Dugar ◽  
Natalia L. Peeck ◽  
Thorsten Bischler ◽  
...  
Keyword(s):  
Campylobacter Jejuni ◽  
Genetic Material ◽  
Simultaneous Detection ◽  
Type Ii ◽  
Rna Detection ◽  
Single Base ◽  
Dna Targeting ◽  
Single Base Resolution ◽  
Rna Complexes

CRISPR-Cas systems recognize foreign genetic material using CRISPR RNAs (crRNAs). In Type II systems, a trans-activating crRNA (tracrRNA) hybridizes to crRNAs to drive their processing and utilization by Cas9. While analyzing Cas9-RNA complexes from Campylobacter jejuni, we discovered tracrRNA hybridizing to cellular RNAs, leading to formation of “noncanonical” crRNAs capable of guiding DNA targeting by Cas9. Our discovery inspired the engineering of reprogrammed tracrRNAs that link the presence of any RNA-of-interest to DNA targeting with different Cas9 orthologs. This capability became the basis for a multiplexable diagnostic platform termed LEOPARD (Leveraging Engineered tracrRNAs and On-target DNAs for PArallel RNA Detection). LEOPARD allowed simultaneous detection of RNAs from different viruses in one test and distinguished SARS-CoV-2 and its D614G variant with single-base resolution in patient samples.

Mapping messenger RNA methylations at single base resolution

2021 ◽  
Vol 63 ◽  
pp. 28-37
Author(s):  
Jie Cao ◽  
Xiao Shu ◽  
Xin-Hua Feng ◽  
Jianzhao Liu
Keyword(s):  
Messenger Rna ◽  
Single Base ◽  
Single Base Resolution
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