scholarly journals Metabolic Pathway Rerouting in Paraburkholderia rhizoxinica Evolved Long-Overlooked Derivatives of Coenzyme F420

2019 ◽  
Vol 14 (9) ◽  
pp. 2088-2094 ◽  
Author(s):  
Daniel Braga ◽  
Daniel Last ◽  
Mahmudul Hasan ◽  
Huijuan Guo ◽  
Daniel Leichnitz ◽  
...  
Keyword(s):  
Metabolic Pathway ◽  
Coenzyme F420 ◽  
Derivatives Of

scholarly journals Metabolic pathway rerouting inParaburkholderia rhizoxinicaevolved long-overlooked derivatives of coenzyme F420

2019 ◽  
Author(s):  
Daniel Braga ◽  
Daniel Last ◽  
Mahmudul Hasan ◽  
Huijuan Guo ◽  
Daniel Leichnitz ◽  
...  
Keyword(s):  
Methanogenic Archaea ◽  
Biosynthetic Gene Cluster ◽  
Phytopathogenic Fungi ◽  
Gram Negative Bacteria ◽  
Biosynthetic Gene ◽  
Coenzyme F420 ◽  
Gram Negative ◽  
Biochemical Processes ◽  
Derivatives Of ◽  
Opening Up

AbstractCoenzyme F420is a specialized redox cofactor with a highly negative redox potential. It supports biochemical processes like methanogenesis, degradation of xenobiotics or the biosynthesis of antibiotics. Although well-studied in methanogenic archaea and actinobacteria, not much is known about F420in Gram-negative bacteria. Genome sequencing revealed F420biosynthetic genes in the Gram-negative, endofungal bacteriumParaburkholderia rhizoxinica, a symbiont of phytopathogenic fungi. Fluorescence microscopy, high-resolution LC-MS, and structure elucidation by NMR demonstrated that the encoded pathway is active and yields unexpected derivatives of F420(3PG-F420). Further analyses of a biogas-producing microbial community showed that these derivatives are more widespread in nature. Genetic and biochemical studies of their biosynthesis established that a specificity switch in the guanylyltransferase CofC re-programmed the pathway to start from 3-phospho-D-glycerate, suggesting a rerouting event during the evolution of F420biosynthesis. Furthermore, the cofactor activity of 3PG-F420was validated, thus opening up perspectives for its use in biocatalysis. The 3PG-F420biosynthetic gene cluster is fully functional inEscherichia coli, enabling convenient production of the cofactor by fermentation.

Fluorinated neuroleptics of the 10-piperazino-10,11-dihydrodibenzo[b,f]thiepin series; 6-Fluoro derivatives of perathiepin, octoclothepin, doclothepin and some related compounds

1979 ◽  
Vol 44 (7) ◽  
pp. 2139-2155 ◽  
Author(s):  
Irena Červená ◽  
Jiřina Metyšová ◽  
Václav Bártl ◽  
Miroslav Protiva
Keyword(s):  
Metabolic Pathway ◽  
Pharmacological Profile ◽  
Fluoro Derivative ◽  
Sedative Effects ◽  
Neuroleptic Agent ◽  
A Minor ◽  
Derivatives Of ◽  
Related Compounds ◽  
Fluoro Derivatives ◽  
Central Depressant

The synthesis of a series of 6-fluoro-10-piperazino-10,11-dihydrodibenzo[b,f]thiepins Ic-IIIc, Vc and VIc is described; these compounds are derivatives of the neuroleptic agents perathiepin (Ia), octoclothepin (IIIa), doclothepin (Va) and their hydroxyethyl analogues IIa and VIa in which the metabolic hydroxylation to position 6 was made impossible by blockade. The synthesis used common procedures via the intermediates VII-XVIII. Fluorination in position 6 does not influence much the pharmacological profile of the compounds indicating that hydroxylation in position 6 is only a minor metabolic pathway. The most interesting substance is the 6-fluoro derivative of octoclothepin (IIIc) which is a potent central depressant and neuroleptic agent with some protraction of the sedative effects.

Simultaneous HPLC determination of four key metabolites in the metabolic pathway for production of 1,3-propanediol from glycerol

2007 ◽  
Vol 65 (9-10) ◽  
pp. 629-632
Author(s):  
H CHEN ◽  
B FANG ◽  
Z HU
Keyword(s):  
Metabolic Pathway ◽  
Hplc Determination

Organic derivatives of tin IV. Reactions of dialkyltin dialkoxides with alkanolamines

1974 ◽  
Vol 63 (2) ◽  
pp. 213-219
Author(s):  
D Gaur
Keyword(s):  
Derivatives Of

Physical Properties of TCNQ Salts with N-Methyl Derivatives of Pyridine

1982 ◽  
Vol 85 (1) ◽  
pp. 257-263 ◽  
Author(s):  
A. Graja ◽  
M. Przybylski ◽  
B. Butka ◽  
R. Swietlik
Keyword(s):  
Physical Properties ◽  
Methyl Derivatives ◽  
Derivatives Of

Mesophases Induced in the Binary Systems Consisting of the 4-Dimethylamino-4-Alkoxy and 4-Nitro-4-Alkoxy Derivatives of N-Benzylideneaniline

1986 ◽  
Vol 131 (3) ◽  
pp. 273-283 ◽  
Author(s):  
N. Homura ◽  
Y. Matsunaga ◽  
M. Suzuki
Keyword(s):  
Binary Systems ◽  
Derivatives Of

Diorganyl Derivatives of Tellurium(IV) (σ-Telluranes of R 2 TeX 2 Type)

2002 ◽  
Vol 23 (2) ◽  
pp. 125-207 ◽  
Author(s):  
Igor D. Sadekov ◽  
Alexander V. Zakharov ◽  
Alexander A. Maksimenko
Keyword(s):  
Derivatives Of
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