Characterization of the two anion-recognition sites of glyceraldehyde-3-phosphate dehydrogenase from Bacillus stearothermophilus by site-directed mutagenesis and chemical modification

Biochemistry ◽

10.1021/bi00177a017 ◽

1994 ◽

Vol 33 (11) ◽

pp. 3260-3265 ◽

Author(s):

C. Corbier ◽

S. Michels ◽

A. J. Wonacott ◽

G. Branlant

Keyword(s):

Chemical Modification ◽

Bacillus Stearothermophilus ◽

Anion Recognition ◽

Site Directed Mutagenesis ◽

Directed Mutagenesis ◽

Recognition Sites

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Characterization of the 2-[(R)-2-Hydroxypropylthio]ethanesulfonate Dehydrogenase fromXanthobacterStrain Py2: Product Inhibition, pH Dependence of Kinetic Parameters, Site-Directed Mutagenesis, Rapid Equilibrium Inhibition, and Chemical Modification†

Biochemistry ◽

10.1021/bi0118005 ◽

2002 ◽

Vol 41 (8) ◽

pp. 2727-2740 ◽

Author(s):

Daniel D. Clark ◽

Scott A. Ensign

Keyword(s):

Chemical Modification ◽

Kinetic Parameters ◽

Ph Dependence ◽

Product Inhibition ◽

Site Directed Mutagenesis ◽

Directed Mutagenesis ◽

Rapid Equilibrium

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Characterization of the phosphotransferase domain of casein kinase II by site-directed mutagenesis and expression in Escherichia coli.

Journal of Biological Chemistry ◽

10.1016/s0021-9258(18)35921-0 ◽

1992 ◽

Vol 267 (33) ◽

pp. 23894-23902

Author(s):

R Jakobi ◽

J.A. Traugh

Keyword(s):

Escherichia Coli ◽

Casein Kinase Ii ◽

Casein Kinase ◽

Site Directed Mutagenesis ◽

Directed Mutagenesis ◽

Expression In Escherichia Coli

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The role of cysteine 206 in allosteric inhibition of Escherichia coli citrate synthase. Studies by chemical modification, site-directed mutagenesis, and 19F NMR.

Journal of Biological Chemistry ◽

10.1016/s0021-9258(18)54766-9 ◽

1991 ◽

Vol 266 (31) ◽

pp. 20709-20713

Author(s):

L.J. Donald ◽

B.R. Crane ◽

D.H. Anderson ◽

H.W. Duckworth

Keyword(s):

Escherichia Coli ◽

Chemical Modification ◽

Citrate Synthase ◽

Site Directed Mutagenesis ◽

19F Nmr ◽

Directed Mutagenesis ◽

Allosteric Inhibition

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Structure-function relationships of cyanobacterial ADP-glucose pyrophosphorylase. Site-directed mutagenesis and chemical modification of the activator-binding sites of ADP-glucose pyrophosphorylase from Anabaena PCC 7120.

Journal of Biological Chemistry ◽

10.1016/s0021-9258(19)51054-7 ◽

1994 ◽

Vol 269 (39) ◽

pp. 24107-24113

Author(s):

Y.Y. Charng ◽

A.A. Iglesias ◽

J. Preiss

Keyword(s):

Chemical Modification ◽

Structure Function ◽

Binding Sites ◽

Site Directed Mutagenesis ◽

Directed Mutagenesis ◽

Anabaena Pcc 7120 ◽

Adp Glucose Pyrophosphorylase ◽

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Characterization of regions of fibronectin besides the arginine-glycine-aspartic acid sequence required for adhesive function of the cell-binding domain using site-directed mutagenesis

Journal of Biological Chemistry ◽

10.1016/s0021-9258(18)98498-x ◽

1991 ◽

Vol 266 (24) ◽

pp. 15938-15943 ◽

Author(s):

S. Aota ◽

T. Nagai ◽

K.M. Yamada

Keyword(s):

Aspartic Acid ◽

Binding Domain ◽

Site Directed Mutagenesis ◽

Directed Mutagenesis ◽

Cell Binding ◽

Arginine Glycine Aspartic Acid ◽

Cell Binding Domain

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Studies on the reaction mechanism of a microbial lipase/acyltransferase using chemical modification and site-directed mutagenesis.

Journal of Biological Chemistry ◽

10.1016/s0021-9258(17)35273-0 ◽

1991 ◽

Vol 266 (2) ◽

pp. 997-1000 ◽

Author(s):

S Hilton ◽

J T Buckley

Keyword(s):

Reaction Mechanism ◽

Chemical Modification ◽

Site Directed Mutagenesis ◽

Directed Mutagenesis ◽

Microbial Lipase

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Characterization of cathepsin B specificity by site-directed mutagenesis. Importance of Glu245 in the S2-P2 specificity for arginine and its role in transition state stabilization.

Journal of Biological Chemistry ◽

10.1016/s0021-9258(18)54140-5 ◽

1993 ◽

Vol 268 (1) ◽

pp. 235-240

Author(s):

S. Hasnain ◽

T. Hirama ◽

C.P. Huber ◽

P. Mason ◽

J.S. Mort

Keyword(s):

Transition State ◽

Cathepsin B ◽

Site Directed Mutagenesis ◽

Directed Mutagenesis ◽

Transition State Stabilization

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Site-directed mutagenesis of Bacillus stearothermophilus lactate dehydrogenase

Biochemical Society Transactions ◽

10.1042/bst0150152 ◽

1987 ◽

Vol 15 (1) ◽

pp. 152-153 ◽

Author(s):

ANTHONY R. CLARKE ◽

DALE B. WIGLEY ◽

DAVID A. BARSTOW ◽

WILLIAM N. CHIA ◽

ADAM D. B. WALDMAN ◽

...

Keyword(s):

Lactate Dehydrogenase ◽

Bacillus Stearothermophilus ◽

Site Directed Mutagenesis ◽

Directed Mutagenesis

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Site-directed mutagenesis of conserved cysteine residues within the .beta. subunit of Escherichia coli nitrate reductase. Physiological, biochemical, and EPR characterization of the mutated enzymes

Biochemistry ◽

10.1021/bi00059a018 ◽

1993 ◽

Vol 32 (8) ◽

pp. 2013-2023 ◽

Author(s):

Valerie Augier ◽

Bruno Guigliarelli ◽

Marcel Asso ◽

Patrick Bertrand ◽

Chantal Frixon ◽

...

Keyword(s):

Escherichia Coli ◽

Nitrate Reductase ◽

Beta Subunit ◽

Site Directed Mutagenesis ◽

Cysteine Residues ◽

Directed Mutagenesis

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Structure-Function Studies of Escherichia coli Biotin Synthase via a Chemical Modification and Site-Directed Mutagenesis Approach

The Journal of Biochemistry ◽

10.1093/oxfordjournals.jbchem.a003028 ◽

2001 ◽

Vol 130 (5) ◽

pp. 627-635 ◽

Author(s):

L. Farh ◽

S.-Y. Hwang ◽

L. Steinrauf ◽

H.-J. Chiang ◽

D. Shiuan

Keyword(s):

Escherichia Coli ◽

Chemical Modification ◽

Structure Function ◽

Site Directed Mutagenesis ◽

Directed Mutagenesis ◽

Biotin Synthase

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