Chemical synthesis and expression of a calmodulin gene designed for site-specific mutagenesis

Biochemistry ◽  
1985 ◽  
Vol 24 (19) ◽  
pp. 5090-5098 ◽  
Author(s):  
Daniel M. Roberts ◽  
Roberto Crea ◽  
Marek Malecha ◽  
Gabriel Alvarado-Urbina ◽  
Ronald H. Chiarello ◽  
...  
Keyword(s):  
Chemical Synthesis ◽  
Site Specific ◽  
Calmodulin Gene

scholarly journals Engineering bromodomains with a photoactive amino acid by engaging ‘Privileged’ tRNA synthetases

2020 ◽  
Vol 56 (25) ◽  
pp. 3641-3644
Author(s):  
Shana Wagner ◽  
Babu Sudhamalla ◽  
Philip Mannes ◽  
Sushma Sappa ◽  
Sam Kavoosi ◽  
...  
Keyword(s):  
Amino Acid ◽  
Chemical Synthesis ◽  
Protein Interactions ◽  
Protein Protein Interactions ◽  
Site Specific ◽  
Trna Synthetases

An improved chemical synthesis, site-specific incorporation and enhanced photo-crosslinking ability of tmdF have been demonstrated in the context of protein–protein interactions.

scholarly journals Chemical synthesis of RNA with site-specific methylphosphonate modifications

Methods ◽  
2016 ◽  
Vol 107 ◽  
pp. 79-88 ◽  
Author(s):  
Sara Flür ◽  
Ronald Micura
Keyword(s):  
Chemical Synthesis ◽  
Site Specific

Erratum: Rapid automated synthesis via diisopropyl phosphoramidite in situ activation. Chemical synthesis and cloning of a calmodulin gene

1986 ◽  
Vol 64 (12) ◽  
pp. 1382-1382
Author(s):  
G. Alvarado-Urbina ◽  
R. Chiarello ◽  
D. Roberts ◽  
G. Vilain ◽  
F. Jurik ◽  
...  
Keyword(s):  
Chemical Synthesis ◽  
Automated Synthesis ◽  
Calmodulin Gene ◽  
In Situ Activation

scholarly journals Serine/threonine ligation-assisted chemical synthesis of HMGA1a protein with site-specific post-translational modifications

2021 ◽  
Vol 2 (3) ◽  
pp. 100777
Author(s):  
Tongyao Wei ◽  
Heng Liu ◽  
Hongxiang Wu ◽  
Fan Pu ◽  
Xuechen Li
Keyword(s):  
Chemical Synthesis ◽  
Post Translational Modifications ◽  
Site Specific

scholarly journals Chemical synthesis of idiotopes. Evidence that antisera to the same JH1 peptide detect multiple binding site-associated idiotopes.

1984 ◽  
Vol 159 (5) ◽  
pp. 1338-1350 ◽  
Author(s):  
M V Seiden ◽  
B Clevinger ◽  
S McMillan ◽  
A Srouji ◽  
R Lerner ◽  
...  
Keyword(s):  
Chemical Synthesis ◽  
Binding Site ◽  
Heavy Chain ◽  
Synthetic Peptides ◽  
Molecular Basis ◽  
Antigen Specificity ◽  
Site Specific ◽  
The Family ◽  
Multiple Binding ◽  
Specific Antisera

In an attempt to better understand the molecular basis of idiotypy, we have generated several site-specific antisera through immunization of animals with synthetic peptides corresponding to the (JH1) heavy chain joining segment 1 of the mouse heavy chain variable (VH) region. These anti-peptide sera identify several idiotypic determinants present on intact hybridoma and myeloma immunoglobulins. Expression of at least three of these idiotopes is correlated with the antigen specificity of the family of immunoglobulins bearing the determinant. Use of synthetic peptides may prove a powerful technique in the generation of molecularly defined antiidiotypic reagents.

P−B Desulfurization: An Enabling Method for Protein Chemical Synthesis and Site-Specific Deuteration

2017 ◽  
Vol 56 (46) ◽  
pp. 14607-14611 ◽  
Author(s):  
Kang Jin ◽  
Tianlu Li ◽  
Hoi Yee Chow ◽  
Han Liu ◽  
Xuechen Li
Keyword(s):  
Chemical Synthesis ◽  
Site Specific
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