Effect of allosteric ligands on the mechanism and stability of nicotinamide-adenine dinucleotide specific isocitrate dehydrogenase from yeast

Biochemistry ◽  
1972 ◽  
Vol 11 (7) ◽  
pp. 1283-1289 ◽  
Author(s):  
Dieter Palm ◽  
Herbert Katzendobler
Keyword(s):  
Isocitrate Dehydrogenase ◽  
Nicotinamide Adenine Dinucleotide ◽  
Nicotinamide Adenine

scholarly journals The effect of pH on the characteristics of the binding of nicotinamide–adenine dinucleotide by nicotinamide–adenine dinucleotide specific isocitrate dehydrogenase from pea mitochondria

1970 ◽  
Vol 116 (5) ◽  
pp. 819-824 ◽  
Author(s):  
G. F. Cox ◽  
D. D. Davies
Keyword(s):  
Isocitrate Dehydrogenase ◽  
Nicotinamide Adenine Dinucleotide ◽  
Maximum Velocity ◽  
Nicotinamide Adenine ◽  
Half Maximum ◽  
Ph Optimum ◽  
Effect Of Ph ◽  
Ph Values ◽  
Metabolic Significance

1. The effect of pH on the Vmax. and concentration of NAD+ at half-maximum velocity at a constant isocitrate concentration was examined, and the results were related to the requirements for binding of H+ ions to the enzyme. 2. The effect of varying the NAD+ concentration on the pH optimum with constant isocitrate concentration was studied. 3. A comparison has been made between the effect of isocitrate concentration on the characteristics of binding of NAD+ and the effect of NAD+ concentration on the characteristics of isocitrate binding at three different pH values. 4. The mechanistic and metabolic significance of these studies is considered.

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