Effect of Adenylic Acid on the Regulatory Nicotinamide-Adenine Dinucleotide Specific Isocitrate Dehydrogenase*

Biochemistry ◽  
1966 ◽  
Vol 5 (3) ◽  
pp. 886-894 ◽  
Author(s):  
B. D. Sanwal ◽  
R. A. Cook
1970 ◽  
Vol 116 (5) ◽  
pp. 819-824 ◽  
Author(s):  
G. F. Cox ◽  
D. D. Davies

1. The effect of pH on the Vmax. and concentration of NAD+ at half-maximum velocity at a constant isocitrate concentration was examined, and the results were related to the requirements for binding of H+ ions to the enzyme. 2. The effect of varying the NAD+ concentration on the pH optimum with constant isocitrate concentration was studied. 3. A comparison has been made between the effect of isocitrate concentration on the characteristics of binding of NAD+ and the effect of NAD+ concentration on the characteristics of isocitrate binding at three different pH values. 4. The mechanistic and metabolic significance of these studies is considered.


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