Fidelity of DNA Polymerase δ Holoenzyme fromSaccharomyces cerevisiae:  The Sliding Clamp Proliferating Cell Nuclear Antigen Decreases Its Fidelity†

Biochemistry ◽  
2003 ◽  
Vol 42 (48) ◽  
pp. 14207-14213 ◽  
Author(s):  
Keiji Hashimoto ◽  
Kikuo Shimizu ◽  
Naomi Nakashima ◽  
Akio Sugino
Keyword(s):  
Dna Polymerase ◽  
Proliferating Cell Nuclear Antigen ◽  
Nuclear Antigen ◽  
Dna Polymerase Δ ◽  
Sliding Clamp ◽  
Proliferating Cell

scholarly journals Identification of a Novel Protein, PDIP38, That Interacts with the p50 Subunit of DNA Polymerase δ and Proliferating Cell Nuclear Antigen

2003 ◽  
Vol 278 (12) ◽  
pp. 10041-10047 ◽  
Author(s):  
Li Liu ◽  
Esther M. Rodriguez-Belmonte ◽  
Nayef Mazloum ◽  
Bin Xie ◽  
Marietta Y. W. T. Lee
Keyword(s):  
Dna Polymerase ◽  
Proliferating Cell Nuclear Antigen ◽  
Nuclear Antigen ◽  
Dna Polymerase Δ ◽  
Proliferating Cell ◽  
Novel Protein

scholarly journals Calf thymus DNA polymerase δ Independent of proliferating cell nuclear antigen (PCNA)

1989 ◽  
Vol 17 (5) ◽  
pp. 1805-1821 ◽  
Author(s):  
Federico Focher ◽  
Max Gassmann ◽  
Peter Hafkmeyer ◽  
Elena Ferrari ◽  
Silvio Spadari ◽  
...  
Keyword(s):  
Dna Polymerase ◽  
Proliferating Cell Nuclear Antigen ◽  
Calf Thymus Dna ◽  
Nuclear Antigen ◽  
Dna Polymerase Δ ◽  
Calf Thymus ◽  
Proliferating Cell

scholarly journals Proliferating Cell Nuclear Antigen Promotes Misincorporation Catalyzed by Calf Thymus DNA Polymerase δ

1996 ◽  
Vol 271 (49) ◽  
pp. 31711-31717 ◽  
Author(s):  
Dmitry Ju. Mozzherin ◽  
Maeve McConnell ◽  
Maxim V. Jasko ◽  
Alexander A. Krayevsky ◽  
Cheng-Keat Tan ◽  
...  
Keyword(s):  
Dna Polymerase ◽  
Proliferating Cell Nuclear Antigen ◽  
Calf Thymus Dna ◽  
Nuclear Antigen ◽  
Dna Polymerase Δ ◽  
Calf Thymus ◽  
Proliferating Cell

scholarly journals Stability of the human polymerase δ holoenzyme and its implications in lagging strand DNA synthesis

2016 ◽  
Vol 113 (13) ◽  
pp. E1777-E1786 ◽  
Author(s):  
Mark Hedglin ◽  
Binod Pandey ◽  
Stephen J. Benkovic
Keyword(s):  
Dna Polymerase ◽  
Proliferating Cell Nuclear Antigen ◽  
Nuclear Antigen ◽  
Okazaki Fragment ◽  
Sliding Clamp ◽  
Dna Lesion ◽  
The Stability ◽  
Proliferating Cell ◽  
Lagging Strand ◽  
Extreme Stability

In eukaryotes, DNA polymerase δ (pol δ) is responsible for replicating the lagging strand template and anchors to the proliferating cell nuclear antigen (PCNA) sliding clamp to form a holoenzyme. The stability of this complex is integral to every aspect of lagging strand replication. Most of our understanding comes fromSaccharomyces cerevisaewhere the extreme stability of the pol δ holoenzyme ensures that every nucleobase within an Okazaki fragment is faithfully duplicated before dissociation but also necessitates an active displacement mechanism for polymerase recycling and exchange. However, the stability of the human pol δ holoenzyme is unknown. We designed unique kinetic assays to analyze the processivity and stability of the pol δ holoenzyme. Surprisingly, the results indicate that human pol δ maintains a loose association with PCNA while replicating DNA. Such behavior has profound implications on Okazaki fragment synthesis in humans as it limits the processivity of pol δ on undamaged DNA and promotes the rapid dissociation of pol δ from PCNA on stalling at a DNA lesion.

scholarly journals Direct Interaction of Proliferating Cell Nuclear Antigen with the Small Subunit of DNA Polymerase δ

2002 ◽  
Vol 277 (27) ◽  
pp. 24340-24345 ◽  
Author(s):  
Xiaoqing Lu ◽  
Cheng-Keat Tan ◽  
Jin-Qiu Zhou ◽  
Min You ◽  
L. Michael Carastro ◽  
...  
Keyword(s):  
Dna Polymerase ◽  
Proliferating Cell Nuclear Antigen ◽  
Direct Interaction ◽  
Small Subunit ◽  
Nuclear Antigen ◽  
Dna Polymerase Δ ◽  
Proliferating Cell
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