Probing the Mechanism of Hamster ArylamineN-Acetyltransferase 2 Acetylation by Active Site Modification, Site-Directed Mutagenesis, and Pre-Steady State and Steady State Kinetic Studies†

Directed Mutagenesis ◽

Steady State Kinetic ◽

Catalytic Acid−Base Groups in Yeast Pyruvate Decarboxylase. 1. Site-Directed Mutagenesis and Steady-State Kinetic Studies on the Enzyme with the D28A, H114F, H115F, and E477Q Substitutions†

Biochemistry ◽

10.1021/bi002855u ◽

2001 ◽

Vol 40 (25) ◽

pp. 7355-7368 ◽

Cited By ~ 39

Author(s):

Min Liu ◽

Eduard A. Sergienko ◽

Fusheng Guo ◽

Jue Wang ◽

Kai Tittmann ◽

...

Keyword(s):

Steady State ◽

Pyruvate Decarboxylase ◽

Kinetic Studies ◽

Directed Mutagenesis ◽

Acid Base ◽

Steady State Kinetic ◽

Identifying the active site base within F420-dependent glucose-6-phosphate dehydrogenase using site-directed mutagenesis, steady-state kinetic methods and pH dependence profiling

Frontiers in Chemistry ◽

10.3389/conf.fchem.2018.01.00011 ◽

2018 ◽

Vol 6 ◽

Author(s):

Lindsay Davis ◽

Kayunta Johnson-Winters

Keyword(s):

Steady State ◽

Active Site ◽

Ph Dependence ◽

Directed Mutagenesis ◽

Kinetic Methods ◽

Steady State Kinetic ◽

Glucose 6 Phosphate Dehydrogenase ◽

Pre-Steady-State Kinetic Studies on the Glu171Gln Active Site Mutant of Adenosylcobalamin-Dependent Glutamate Mutase†

Biochemistry ◽

10.1021/bi020596y ◽

2002 ◽

Vol 41 (52) ◽

pp. 15803-15809 ◽

Cited By ~ 3

Author(s):

Prashanti Madhavapeddi ◽

David P. Ballou ◽

E. Neil G. Marsh

Keyword(s):

Steady State ◽

Active Site ◽

Kinetic Studies ◽

Glutamate Mutase ◽

Steady State Kinetic ◽

Expression, Site-Directed Mutagenesis, and Steady State Kinetic Analysis of the Terminal Thioesterase Domain of the Methymycin/Picromycin Polyketide Synthase†

Biochemistry ◽

10.1021/bi026006d ◽

2002 ◽

Vol 41 (42) ◽

pp. 12590-12597 ◽

Cited By ~ 46

Author(s):

Hongxiang Lu ◽

Shiou-Chuan Tsai ◽

Chaitan Khosla ◽

David E. Cane

Keyword(s):

Steady State ◽

Kinetic Analysis ◽

Polyketide Synthase ◽

Directed Mutagenesis ◽

Steady State Kinetic ◽

Expression Site ◽

DNA Polymerase β: Pre-Steady-State Kinetic Analyses of dATPαS Stereoselectivity and Alteration of the Stereoselectivity by Various Metal Ions and by Site-Directed Mutagenesis†

Biochemistry ◽

10.1021/bi010646j ◽

2001 ◽

Vol 40 (30) ◽

pp. 9014-9022 ◽

Cited By ~ 28

Author(s):

Jia Liu ◽

Ming-Daw Tsai

Keyword(s):

Steady State ◽

Metal Ions ◽

Dna Polymerase ◽

Directed Mutagenesis ◽

Dna Polymerase Β ◽

Steady State Kinetic ◽

Saturation mutagenesis, complement selection, and steady-state kinetic studies illuminate the roles of invariant residues in active site loop I of the hypoxanthine phosphoribosyltransferase from Trypanosoma cruzi

Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics ◽

10.1016/s1570-9639(04)00027-5 ◽

2004 ◽

Vol 1699 (1-2) ◽

pp. 87-94 ◽

Cited By ~ 4

Author(s):

A BUTTERWORTH

Keyword(s):

Steady State ◽

Trypanosoma Cruzi ◽

Active Site ◽

Kinetic Studies ◽

Saturation Mutagenesis ◽

Hypoxanthine Phosphoribosyltransferase ◽

Steady State Kinetic ◽

Complement Selection ◽

Site-directed mutagenesis and steady-state kinetic analysis of mutant enzymes of human adenylate kinase

IUBMB Life ◽

10.1080/15216549800204202 ◽

1998 ◽

Vol 46 (4) ◽

pp. 673-680

Author(s):

Takanori Ayabe ◽

Seung Kyu Park ◽

Hiroyuki Nagahama ◽

Hideharu Maruyama ◽

Michihiro Sumida ◽

...

Keyword(s):

Steady State ◽

Kinetic Analysis ◽

Adenylate Kinase ◽

Directed Mutagenesis ◽

Steady State Kinetic ◽

Steady-State Kinetic Studies of Binding and Catalysis by Ribonuclease T2: A Microenvironmental Survey of the Active Site by Using a Series of Adenosine-3′- and-5′-Alkylphosphates

The Journal of Biochemistry ◽

10.1093/oxfordjournals.jbchem.a134494 ◽

1983 ◽

Keyword(s):

Steady State ◽

Active Site ◽

Kinetic Studies ◽

Steady State Kinetic ◽

Pre-Steady-State and Steady-State Kinetic Studies on Transcription Initiation Catalyzed by T7 RNA Polymerase and Its Active-Site Mutants K631R and Y639F†

Biochemistry ◽

10.1021/bi9805801 ◽

1998 ◽

Vol 37 (45) ◽

pp. 15958-15964 ◽

Cited By ~ 7

Author(s):

A-Young Moon Woody ◽

Patricia A. Osumi-Davis ◽

Meenaxi M. Hiremath ◽

Robert W. Woody

Keyword(s):

Steady State ◽

Rna Polymerase ◽

Active Site ◽

Transcription Initiation ◽

Kinetic Studies ◽

T7 Rna Polymerase ◽

Steady State Kinetic ◽

Active Site Mutants ◽

A reaction mechanism from steady state kinetic studies for O-acetylserine sulfhydrylase from Salmonella typhimurium LT-2.

Journal of Biological Chemistry ◽

10.1016/s0021-9258(17)33649-9 ◽

1976 ◽

Vol 251 (7) ◽

pp. 2023-2029

Author(s):

P F Cook ◽

R T Wedding

Keyword(s):

Steady State ◽

Reaction Mechanism ◽

Salmonella Typhimurium ◽

Kinetic Studies ◽

Steady State Kinetic ◽