Imaging of cell/substrate contacts on polymers by total internal reflection fluorescence microscopy

1994 ◽  
Vol 10 (1) ◽  
pp. 26-31 ◽  
Author(s):  
J. S. Burmeister ◽  
G. A. Truskey ◽  
J. L. Yarbrough ◽  
W. M. Reichert
Keyword(s):  
Fluorescence Microscopy ◽  
Total Internal Reflection ◽  
Internal Reflection ◽  
Total Internal Reflection Fluorescence ◽  
Cell Substrate

Submembrane Events In Triggerable Cells Studied by Total Internal Reflection Fluorescence Microscopy.

1999 ◽  
Vol 5 (S2) ◽  
pp. 1052-1053
Author(s):  
D. Axelrod ◽  
L.M. Johns ◽  
E.S. Levitan ◽  
G.M. Omann ◽  
R.W. Holz
Keyword(s):  
Fluorescence Microscopy ◽  
Total Internal Reflection ◽  
Chromaffin Cells ◽  
Secretory Granules ◽  
Internal Reflection ◽  
Total Internal Reflection Fluorescence ◽  
Cell Substrate ◽  
Before And After ◽  
Cell Medium ◽  
Very High

We have studied the dynamics of certain key processes near the plasma membrane inside two types of chemically-triggerable living cells using total internal reflection fluorescence microscopy (TIRFM). In TIRFM, a laser beam is incident upon the cell/glass-substrate interface from the glass side at an angle greater than the critical angle for total internal reflection. This creates an exponentially decaying evanescent field in the cell medium (with a characteristic depth of > 100 nm) capable of exciting fluorescence selectively from the membrane-proximal regions at cell/substrate contacts. Various ways of setting up the optics for such a system are discussed, involving the use of either prisms or very high aperture objectives.In one application of TIRFM, the motion of adrenalin-containing secretory granules in the immediate submembrane region of chromaffin cells is examined before and after chemical stimulation that causes the granules to release their contents to the cell exterior.

scholarly journals Fast high-resolution 3D total internal reflection fluorescence microscopy by incidence angle scanning and azimuthal averaging

2014 ◽  
Vol 111 (48) ◽  
pp. 17164-17169 ◽  
Author(s):  
Jérôme Boulanger ◽  
Charles Gueudry ◽  
Daniel Münch ◽  
Bertrand Cinquin ◽  
Perrine Paul-Gilloteaux ◽  
...  
Keyword(s):  
High Resolution ◽  
Fluorescence Microscopy ◽  
Total Internal Reflection ◽  
Incidence Angle ◽  
Internal Reflection ◽  
Total Internal Reflection Fluorescence

scholarly journals Microplate-compatible total internal reflection fluorescence microscopy for receptor pharmacology

2013 ◽  
Vol 102 (19) ◽  
pp. 193702 ◽  
Author(s):  
Minghan Chen ◽  
Natalya V. Zaytseva ◽  
Qi Wu ◽  
Min Li ◽  
Ye Fang
Keyword(s):  
Fluorescence Microscopy ◽  
Total Internal Reflection ◽  
Internal Reflection ◽  
Total Internal Reflection Fluorescence ◽  
Receptor Pharmacology
Sign in / Sign up

Export Citation Format

Share Document