Submerged Citric Acid Fermentation of Sugar Beet Molasses. Effect of Ferrocyanide Control

Author(s):  
D. S. Clark
1962 ◽  
Vol 8 (1) ◽  
pp. 133-136 ◽  
Author(s):  
D. S. Clark

A study of the internal structure of Aspergillus niger pellets, grown during submerged citric acid fermentation of ferrocyanide-treated beet molasses, was made using botanical microtechniques. Under optimum fermentation conditions, each pellet developed as a round mass of mycelium of uniform consistency during the first 24 hours of the fermentation (mash sparged with air); subsequently (mash sparged with oxygen), a dense crust of growth formed at the periphery of the pellet and autolysis of cells at the center began. At the end of fermentation (140 hours), the pellet consisted of a shell of mycelium occupying less than 50% of the pellet volume. Changes in fermentation conditions were reflected in the density of peripheral growth and in the rate and extent of autolysis.


1965 ◽  
Vol 11 (1) ◽  
pp. 13-16
Author(s):  
A. ČEJKOVÁ ◽  
J. RYBÁŘOVÁ ◽  
M. ŠESTÁKOVÁ

1963 ◽  
Vol 9 (11) ◽  
pp. 259-262
Author(s):  
A. ČEJKOVÁ ◽  
M. VOJKOVSKÁ ◽  
J. RYBÁŘOVÁ

1946 ◽  
Vol 52 (5) ◽  
pp. 555-564 ◽  
Author(s):  
Philipp Gerhardt ◽  
W. W. Dorrell ◽  
I. L. Baldwin

1961 ◽  
Vol 7 (4) ◽  
pp. 447-453 ◽  
Author(s):  
D. S. Clark ◽  
C. P. Lentz

Recirculation of oxygen during submerged citric acid fermentation of ferrocyanide-treated beet molasses by Aspergillus niger was successful. Results obtained in 36-liter glass tower fermenters showed that neither rate of production nor total yield of citric acid was affected. No treatment of the recirculated gas other than carbon dioxide removal was required. At both low and high oxygen flow rates, increased oxygen pressure (up to 1.7 atm) markedly increased initial rate of production and total yield of citric acid. Oxygen pressure above 1.7 atm decreased citric acid yield. Within the limits tested, the effect of oxygen pressure was independent of total pressure.


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