Paramagnetically Induced Residual Dipolar Couplings for Solution Structure Determination of Lanthanide Binding Proteins

2002 ◽  
Vol 124 (19) ◽  
pp. 5581-5587 ◽  
Author(s):  
Renato Barbieri ◽  
Ivano Bertini ◽  
Gabriele Cavallaro ◽  
Yong-Min Lee ◽  
Claudio Luchinat ◽  
...  
Keyword(s):  
Structure Determination ◽  
Solution Structure ◽  
Binding Proteins ◽  
Residual Dipolar Couplings ◽  
Dipolar Couplings ◽  
Solution Structure Determination

Residual Dipolar Couplings in Structure Determination of Biomolecules

ChemInform ◽  
2004 ◽  
Vol 35 (45) ◽  
Author(s):  
J. H. Prestegard ◽  
C. M. Bougault ◽  
A. I. Kishore
Keyword(s):  
Structure Determination ◽  
Residual Dipolar Couplings ◽  
Dipolar Couplings

scholarly journals NMR Spectroscopic Determination of the Solution Structure of a Branched Nucleic Acid from Residual Dipolar Couplings by Using Isotopically Labeled Nucleotides

2004 ◽  
Vol 43 (2) ◽  
pp. 187-192 ◽  
Author(s):  
Bernd N. M. van Buuren ◽  
Jürgen Schleucher ◽  
Valentin Wittmann ◽  
Christian Griesinger ◽  
Harald Schwalbe ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Solution Structure ◽  
Residual Dipolar Couplings ◽  
Dipolar Couplings ◽  
Spectroscopic Determination

scholarly journals Structure calculation, refinement and validation usingCcpNmr Analysis

2015 ◽  
Vol 71 (1) ◽  
pp. 154-161 ◽  
Author(s):  
Simon P. Skinner ◽  
Benjamin T. Goult ◽  
Rasmus H. Fogh ◽  
Wayne Boucher ◽  
Tim J. Stevens ◽  
...  
Keyword(s):  
Structure Determination ◽  
Solution Structure ◽  
Residual Dipolar Couplings ◽  
Structure Calculation ◽  
Biological Macromolecules ◽  
Nmr Structure Determination ◽  
Determination Process ◽  
The Many ◽  
Nmr Restraints

CcpNmr Analysisprovides a streamlined pipeline for both NMR chemical shift assignment and structure determination of biological macromolecules. In addition, it encompasses tools to analyse the many additional experiments that make NMR such a pivotal technique for research into complex biological questions. This report describes howCcpNmr Analysiscan seamlessly link together all of the tasks in the NMR structure-determination process. It details each of the stages from generating NMR restraints [distance, dihedral, hydrogen bonds and residual dipolar couplings (RDCs)], exporting these to and subsequently re-importing them from structure-calculation software (such as the programsCYANAorARIA) and analysing and validating the results obtained from the structure calculation to, ultimately, the streamlined deposition of the completed assignments and the refined ensemble of structures into the PDBe repository. Until recently, such solution-structure determination by NMR has been quite a laborious task, requiring multiple stages and programs. However, with the new enhancements toCcpNmr Analysisdescribed here, this process is now much more intuitive and efficient and less error-prone.

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