Mechanistic Investigation of Charge Separation in Electrospray Ionization using Microparticles to Record Droplet Charge State

2020 ◽  
Vol 31 (10) ◽  
pp. 2044-2052
Author(s):  
Jiuzhi Gao ◽  
Daniel E. Austin
Keyword(s):  
Electrospray Ionization ◽  
Charge State ◽  
Charge Separation ◽  
Mechanistic Investigation ◽  
Droplet Charge

Comparison of a + T-Rich Oligonucleotides with and without Self-Complementary Sequence Using Ion-Pair Reversed-Phase High-Performance Liquid Chromatography/Tandem Electrospray Ionization Mass Spectrometry

2005 ◽  
Vol 11 (1) ◽  
pp. 83-91 ◽  
Author(s):  
Renfang Song ◽  
Wenbing Zhang ◽  
Huayong Chen ◽  
Huimin Ma ◽  
Yulian Dong ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Electrospray Ionization ◽  
Charge State ◽  
Reversed Phase ◽  
Ion Pair ◽  
Buffer System ◽  
Ionization Mass ◽  
Complementary Sequence ◽  
Complementary Sequences ◽  
Lower Charge

Both A + T-rich oligonucleotides with and without self-complementary sequences were analyzed using ion-pair reversed-phase liquid chromatography/electrospray ionization mass spectrometry (IP-RP-HPLC/ESI-MS) by tryethylammonium acetate (TEAA) and hexafluoroisopropanol (HFIP) buffer systems to study the characteristics of their retention behavior and electrospray ionization tandem mass spectrometry (ESI-MS/MS) response. The results indicated that the chain length had the same effect on the retention of A + T-rich oligonucleotides in both of TEAA and HFIP buffer systems but the sequence had a different impact on the retention in the two buffer systems. A + T-rich oligonucleotides with a self-complementary sequence were much shorter than those without in the TEAA buffer system whereas a slight difference was observed in the HFIP buffer system. Similar total ion current (TIC) intensity was observed both in oligonucleotides with or without self-complementary sequence. The opposite trend of a change in the TIC intensities with increasing chain length were observed in both the TEAA and HFIP buffer systems. A lower charge state was predominant in the TEAA buffer system whereas a higher charge state was mainly distributed in the HFIP buffer system. The oligonucleotides without self-complementary sequences had a higher charge state than those with self-complementary sequences. A- and T- are more esily formed at a higher charge state whereas the sequence fragments will be formed more easily at a lower charge state in both A + T-rich oligonucleotides with and without self-complementary sequences.

Affecting Protein Charge State Distributions in Nano-Electrospray Ionization via In-Spray Solution Mixing Using Theta Capillaries

2014 ◽  
Vol 86 (9) ◽  
pp. 4581-4588 ◽  
Author(s):  
Christine M. Fisher ◽  
Anastasia Kharlamova ◽  
Scott A. McLuckey
Keyword(s):  
Electrospray Ionization ◽  
Charge State ◽  
Protein Charge ◽  
Spray Solution ◽  
Charge State Distributions

scholarly journals Effect of protein stabilization on charge state distribution in positive- and negative-ion electrospray ionization mass spectra

2007 ◽  
Vol 18 (9) ◽  
pp. 1605-1611 ◽  
Author(s):  
Stephen J. Watt ◽  
Margaret M. Sheil ◽  
Jennifer L. Beck ◽  
Pavel Prosselkov ◽  
Gottfried Otting ◽  
...  
Keyword(s):  
Electrospray Ionization ◽  
Charge State ◽  
Mass Spectra ◽  
Charge State Distribution ◽  
Protein Stabilization ◽  
Negative Ion ◽  
Ionization Mass ◽  
State Distribution
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