Purification and Partial Biochemical Characterization of Polyphenol Oxidase from Mango (Mangifera indica cv. Manila)

2014 ◽  
Vol 62 (40) ◽  
pp. 9832-9840 ◽  
Author(s):  
Gisela Palma-Orozco ◽  
Norma A. Marrufo-Hernández ◽  
José G. Sampedro ◽  
Hugo Nájera
Keyword(s):  
Polyphenol Oxidase ◽  
Biochemical Characterization ◽  
Mangifera Indica

Purification and partial biochemical characterization of polyphenol oxidase from mamey (Pouteria sapota)

2011 ◽  
Vol 72 (1) ◽  
pp. 82-88 ◽  
Author(s):  
Gisela Palma-Orozco ◽  
Alicia Ortiz-Moreno ◽  
Lidia Dorantes-Álvarez ◽  
José G. Sampedro ◽  
Hugo Nájera
Keyword(s):  
Polyphenol Oxidase ◽  
Biochemical Characterization

scholarly journals Study on Purification and Characterization of Polyphenol Oxidase from Acetes chinensis

Molecules ◽  
2021 ◽  
Vol 26 (24) ◽  
pp. 7545
Author(s):  
Jianyou Zhang ◽  
Guangcheng Zhou ◽  
Lifeng Fei ◽  
Lifan Chen ◽  
Lei Sun ◽  
...  
Keyword(s):  
Polyphenol Oxidase ◽  
Biochemical Characterization ◽  
Specific Activity ◽  
Deae Cellulose ◽  
Enzymatic Reactions ◽  
Inhibitory Effects ◽  
Purification And Characterization ◽  
Effective Prevention ◽  
Ammonium Sulphate Precipitation

Acetes chinensis (belonging to the Decapoda Sergestidae genus) is widely distributed in East Asian waters and is extremely widespread and present in the shallow coastal areas of China. Polyphenol oxidase (PPO), which was extracted from Acetes chinensis, was purified in a four-step procedure involving phosphate-buffered saline treatment, ammonium sulphate precipitation, DEAE-Cellulose chromatography, and Phenyl-Sepharose HP chromatography, and then, its biochemical characterization was measured. The specific activity of the purified enzyme was increased to 643.4 U/mg, which is a 30.35 times increase in purification, and the recovery rate was 17.9%. L-dopa was used as the substrate, the enzymatic reactions catalyzed by PPO conformed to the Michaelis equation, the maximum reaction velocity was 769.23 U/mL, and the Michaelis constant Km was 0.846 mmol/L. The optimal pH of PPO from Acetes chinensis was 7.5, and the optimal temperature was 35 °C. The metal ions experiment showed that Mn2+ and K+ could enhance the activity of PPO; that Ba2+ and Ca2+ could inhibit the activity of PPO; and that Cu2+ had a double effect on PPO, increasing the PPO activity at low concentrations and inhibiting the PPO activity at high concentrations. The inhibitor experiment showed that the inhibitory effects of EDTA and kojic acid were weak and that ascorbic acid and sodium pyrophosphate had good inhibitory effects. The purification and characterization of Acetes chinensis serve as guidelines for the prediction of enzyme behavior, leading to effective prevention of enzymatic browning during processing.

Purification and biochemical characterization of polyphenol oxidase from soursop (Annona muricata L. ) and its inactivation by microwave and ultrasound treatments

2019 ◽  
Vol 43 (3) ◽  
pp. e12770 ◽  
Author(s):  
Gisela Palma-Orozco ◽  
Norma A. Marrufo-Hernández ◽  
Ileana Tobías ◽  
Hugo Nájera
Keyword(s):  
Polyphenol Oxidase ◽  
Biochemical Characterization ◽  
Annona Muricata

Purification and Biochemical Characterization of Polyphenol oxidase of African Bush Mango (Irvingia gabonensis) Fruit Peel

2021 ◽  
pp. 102119
Author(s):  
Catherine Joke Adeseko ◽  
David Morakinyo Sanni ◽  
Sule Ola Salawu ◽  
Ige Joseph Kade ◽  
Samuel Olufemi Bamidele ◽  
...  
Keyword(s):  
Polyphenol Oxidase ◽  
Biochemical Characterization ◽  
Fruit Peel ◽  
Bush Mango ◽  
Irvingia Gabonensis

Biochemical Characterization of Wheat Polyphenol Oxidase Activity in Genebank Accessions

2021 ◽  
Vol 34 (1) ◽  
pp. 75-78
Author(s):  
Kalyani Srinivasan ◽  
Reshma Shaheen ◽  
Aradhana Mishra ◽  
Kumar MB Arun ◽  
Eldho Varghese ◽  
...  
Keyword(s):  
Polyphenol Oxidase ◽  
Oxidase Activity ◽  
Biochemical Characterization ◽  
Polyphenol Oxidase Activity

PURIFICATION AND BIOCHEMICAL CHARACTERIZATION OF IONICALLY UNBOUND POLYPHENOL OXIDASE FROMMusa paradisiacaLEAF

2011 ◽  
Vol 41 (2) ◽  
pp. 187-200 ◽  
Author(s):  
Sanjeev Kumar Diwakar ◽  
Sarad Kumar Mishra
Keyword(s):  
Polyphenol Oxidase ◽  
Biochemical Characterization

Plant Pathology Diagnosed by X-Ray Microanalysis

1987 ◽  
Vol 45 ◽  
pp. 974-975
Author(s):  
J. H. Resau ◽  
N. Howell ◽  
S. H. Chang
Keyword(s):  
Electron Microscopy ◽  
Plant Pathology ◽  
Biochemical Characterization ◽  
Nutrient Deficiency ◽  
Green Leaf ◽  
Parasitic Infestation ◽  
X Ray

Spinach grown in Texas developed “yellow spotting” on the peripheral portions of the leaves. The exact cause of the discoloration could not be determined as there was no evidence of viral or parasitic infestation of the plants and biochemical characterization of the plants did not indicate any significant differences between the yellow and green leaf portions of the spinach. The present study was undertaken using electron microscopy (EM) to determine if a micro-nutrient deficiency was the cause for the discoloration.Green leaf spinach was collected from the field and sent by express mail to the EM laboratory. The yellow and equivalent green portions of the leaves were isolated and dried in a Denton evaporator at 10-5 Torr for 24 hrs. The leaf specimens were then examined using a JEOL 100 CX analytical microscope. TEM specimens were prepared according to the methods of Trump et al.

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