How to Adapt Scatchard Plot for Graphically Addressing Cooperativity in Multicomponent Self-Assemblies

2006 ◽  
Vol 110 (15) ◽  
pp. 7783-7792 ◽  
Author(s):  
Josef Hamacek ◽  
Claude Piguet
Keyword(s):  
Scatchard Plot

Scatchard Plot and Heterogeneity in Binding Affinity of Labeled and Unlabeled Ligand

1975 ◽  
Vol 21 (12) ◽  
pp. 1769-1773 ◽  
Author(s):  
H J G Hollemans ◽  
R M Bertina
Keyword(s):  
Binding Affinity ◽  
Binding Sites ◽  
Mathematical Expression ◽  
Molar Concentration ◽  
Scatchard Plot ◽  
Affinity Constant

Abstract In saturation analysis the Scatchard plot is a generally accepted method for calculation of the affinity constant, K, and the molar concentration, q, of the binder. However, in a system where the K's for the labeled and unlabeled ligand are unequal, a nonlinear plot can be obtained from which incorrect values for K and q may be calculated. This paper mathematically explains how the plot may deviate and under which conditions there will be a maximum in the curve. When the binding sites are homogeneous, the coordinates of this maximum can be used to calculate K and q. A general mathematical expression is derived on the basis of which a linear curve can be constructed for calculation of q and K, which is valid even when affinity for the labeled and unlabeled ligand is not identical.

Simplified Scatchard-Plot Assay for Estrogen Receptor in Human Breast Tumor

1975 ◽  
Vol 21 (12) ◽  
pp. 1725-1730 ◽  
Author(s):  
Roy B Johnson ◽  
Robert M Nakamura ◽  
Rose Mary Libby
Keyword(s):  
Estrogen Receptor ◽  
Breast Tumor ◽  
Tumor Tissue ◽  
Competitive Inhibition ◽  
Scatchard Plot ◽  
Human Tissues ◽  
Human Breast ◽  
Human Breast Tumor ◽  
Pregnant Rabbit ◽  
Before And After

Abstract In this two-point Scatchard-plot assay, with which a test of competitive inhibition is combined, the sample is mechanically homogenized in a buffer containing dithiothreitol, ultracentrifuged to obtain a fat-free cytosol, the protein content of which is then adjusted, and free and bound labeled estradiol are separated with dextrancoated charcoal after overnight incubation. We tested the method for precision and reliability by assaying such cytosols from pregnant rabbit uteri before and after dilution with kidney cytosol, and by assaying several other target and nontarget animal and human tissues. The Scatchard plot data were more reliable than tests for percent inhibition of binding by a competitor (diethylstilbestrol). For a tumor tissue to be judged positive it must bind at least 8 fmol of estradiol per milligram of protein and have a Kd of 0.1 to 5 x 10-10 mol/liter. Some nontarget tissues showed less than 70% inhibition by 104-fold concentrations (over labeled estradiol) of inhibitor. Of 19 breast-tumor specimens, seven were found to be positive.

Scatchard Plot Analysis of Ligand-Erythrocyte Interactions

1978 ◽  
Vol 67 (9) ◽  
pp. 1344-1345 ◽  
Author(s):  
D.L. Parsons ◽  
J.J. Vallner
Keyword(s):  
Scatchard Plot ◽  
Plot Analysis ◽  
Scatchard Plot Analysis

scholarly journals An Improved Procedure for Binding Parameter Fitting from Scatchard Plot

1981 ◽  
Vol 101 (5) ◽  
pp. 443-451
Author(s):  
MAGOBEI YAMAMOTO ◽  
HATSUMI AKI ◽  
TOSHIKI WAKABAYASHI
Keyword(s):  
Scatchard Plot ◽  
Binding Parameter ◽  
Parameter Fitting

Scatchard Plot

2016 ◽  
Author(s):  
Richard G. Jones ◽  
Christopher K. Ober ◽  
Philip Hodge ◽  
Pavel Kratochvíl ◽  
Graeme Moad ◽  
...  
Keyword(s):  
Scatchard Plot
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